Solid-state NMR studies of chromatin structure and dynamics

Abstract

Understanding the dynamic organization of chromatin is indispensable as it provides crucial information to delineate the cellular processes that it mediates -DNA replication, transcription, and repair. The nucleosome core particle (NCP) is the fundamental unit of eukaryotic chromatin composed of four core histone proteins (H2A, H2B, H3, and H4) organizing ~147 bp of DNA. While the structure of the nucleosome is understood predominantly using X-ray crystallography and cryo-EM, quantitative information about nucleosome dynamics remains Telo NCP nucleosome core particle formed by 145 bp telomeric DNA TEMED tetramethyl ethylene diamine ContentsSupervisor Declaration Statement .