Solid-state synthesis of unsaturated chitosan derivatives to design 3D structures through two-photon-induced polymerization

Акопова, Т. А.; Timashev, P. S.; Демина, Т. С.; Bardakova, Kseniia N.; Минаев, Н. В.; Бурдуковский, В. Ф.; Cherkaev, G. V.; Vladimirov, L. V.; Istomin, A. V.; Svidchenko, Evgeniya A.; Сурин, Н. М.; Баграташвили, В.Н.

doi:10.1016/j.mencom.2015.07.017

Cited by 26 publications

(15 citation statements)

References 19 publications

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“…DLP was used together with a chitosan/PCL/PEGDA resin to fabricate scaffolds with feature sizes of~100 µm [185]. Chitosan/allyl bromide produced via a solvent free process was used for TPP at a resolution of 400 nm [228]. Besides the various fabrication techniques, chitosan can be combined with bioceramic hydroxyapatite to print hollow 3D scaffolds for improved vascularization and mechanical properties appropriate for bone tissue engineering [229].…”

Section: Structural and Mechanical Propertiesmentioning

confidence: 99%

“…Hyaluronic acid (4.3.1) [207][208][209]; Inclusion of GelMA, feature size 500 µm [210]; Cryogel E: 2-2.5 kPa [211]; Post-curing via UV, E: 1.3-10.6 kPa [213] Feature size 300 µm (SLA) [214]; Compressive E: 780 kPa [215] Cartilage tissue engineering and human adipose stem cells [215]; stromal cell elongation and drug screening [209]; retinal cell culturing [216]; hMSCs [217]; human adipose progenitor and stromal cells [211]; Schwann cells [219] Chitosan (4.3.2) [222,223]; Feature size 50 µm [224] [225,226]; Feature size 50 µm (SLA), E: 160-680 kPa [173]; Feature size 400 nm (TPP) [228]; Inclusion of HA [229] Anti-bacterial [230,231]; wound dressings [232]; skin tissue engineering [231]; bone tissue engineering [229]; pluripotent stem cells for neural tissue engineering [233]; articular cartilage tissue engineering [234]; skin constructs [235] Alginate (4.3.3)…”

Section: ) Applicationsmentioning

confidence: 99%

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Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Section: Structural and Mechanical Propertiesmentioning

confidence: 99%

Section: ) Applicationsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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“…3D биодеградируемый скаффолд был создан с использованием микростереолитографической техники путем комбинации высокомолекулярного модифицированного хитозана (80 кДа, степень ацети- [6] и высокомолекулярной гиалуроновой кислоты [7], соотношение по массе 3:1.…”

Section: материалы и методы исследованияunclassified

Time-Dependent Effect of 3d Biodegradable Scaffold Transplantation on the Functional Outcome of C57bl/6 Mice in the Therapy of Severe Open Traumatic Brain Injury

Balyabin¹,

Tikhobrazova²,

Shelchkova³

et al. 2017

IJAFR (МЖПФИ)

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3Институт фотонных технологий, Федеральный научно-исследовательский центр «Кристаллография и фотоника» РАН, Москва, Троицк В работе на модели тяжелой открытой черепно-мозговой травмы проведено сравнительное изучение влияния времени имплантации 3D биодеградируемого скаффолда на восстановление поведенческих, ког-нитивных и иммунологических функций мышей в посттравматическом периоде. В работе показано, что имплантирование скаффолда через 1 неделю после ЧМТ, в сравнении с трансплантацией на 5 сутки, ока-зывает более быстрое восстановление структуры поведения животных и функций памяти. Трансплантация скаффолда в очаг повреждения на 7 сутки, в отличие от имплантации на 5 день, не вызывала активации воспалительных процессов в крови животных, а также не приводила к развитию аутоиммунных реакций. Таким образом, наиболее оптимальным временем имплантирования 3D скаффолда для функционального восстановления животных после тяжелой ЧМТ являлся 7 день после нанесения травмы. A comparative study of the influence of the time point implantation of 3D biodegradable scaffold on the restoration of behavioral, cognitive and immunological functions of mice in the posttraumatic period was carried out on the model of severe open traumatic brain injury. It is shown that scaffold implantation one week after TBI, in comparison with transplantation on 5th day, provides a faster recovery of animal behavior and memory functions. Activation of inflammatory processes in the blood of animals as well as the development of autoimmune reactions was not observed when scaffold was implanted into the lesion cavity on 7 day, unlike transplantation after5 days. Thus, the most optimal time point for 3D scaffold implantation for functional recovery of animals after severe traumatic brain injury was been on 7 day after injury.

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“…By changing the chemical functional group of chitosan, synthesized solvent-free N-alkylation of chitosan could be used for multi-photon polymerization. 59 Successfully seeded with cells, the 3D multi-photon fabricated chitosan scaffolds were found to be biocompatible and promising for future tissue engineering applications. Multi-photon polymerization was also used to fabricate polylactic acid (PLA) scaffolds for neurological regenerative medicine.…”

Section: D Structure Fabrication Using Multi-photon Polymerizationmentioning

confidence: 99%

Laser-assisted biofabrication in tissue engineering and regenerative medicine

et al. 2016

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Controlling the spatial arrangement of biomaterials and living cells provides the foundation for fabricating complex biological systems. Such level of spatial resolution (less than 10 lm) is difficult to be obtained through conventional cell processing techniques, which lack the precision, reproducibility, automation, and speed required for the rapid fabrication of engineered tissue constructs. Recently, laserassisted biofabrication techniques are being intensively developed with the use of computer-aided processes for patterning and assembling both living and nonliving materials with prescribed 2D or 3D organization. In this review, we discuss laser-assisted fabrication methods, including laser tweezers, multi-photon polymerization, laser-induced forward transfer (LIFT), matrix assisted pulsed laser evaporation (MAPLE), and laser ablation as well as their applications in biological science and biomedical engineering. These advanced technologies enable the precise manipulation of in vitro cellular microenvironments and the ability to engineer functional tissue constructs with high complexity and heterogeneity, which serve in regenerative medicine, pharmacology, and basic cell biology studies.

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Solid-state synthesis of unsaturated chitosan derivatives to design 3D structures through two-photon-induced polymerization

Cited by 26 publications

References 19 publications

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

Time-Dependent Effect of 3d Biodegradable Scaffold Transplantation on the Functional Outcome of C57bl/6 Mice in the Therapy of Severe Open Traumatic Brain Injury

Laser-assisted biofabrication in tissue engineering and regenerative medicine

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