Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed

Abstract

The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.

Modulation of growth, skin mucus and serum immunities, and disease resistance of Nile tilapia fed host‐associated probiotic (Lactobacillus paracasei l61‐27b)

“…In other words, hostderived probiotics can indirectly enhance the digestibility of nutrients in fish intestines by competing with pathogenic microorganisms and inhibiting their harmful impact on intestinal health (Van Doan et al, 2020;Roques et al, 2020). Additionally, it has been reported that solid-state treatment with L. paracasei could improve nutrient bioavailability in granular fish feed (Lukic et al, 2019).…”

“…Intensive fish farming in closed culture units, in which the nature environment is lacking, is an important mission in ensuring a sufficient amount of nutrients for fish production and growth (Craig et al, 2002;Lukic et al, 2019). Favourable microbiota seems to be a more cost-effective and environmentally friendly way to change the nutritional properties of completed fish feed (Hasan & Banerjee, 2020;Sharifuzzaman & Austin, 2017;.…”

Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties

“…paracasei BGHN14 (isolated from homemade hard cheese traditionally manufactured in the coastal region of Montenegro) in combination with Lb. rhamnosus BGT10 drastically reduced neutral lipid amounts on the surface of granular fish feed (Lukic et al, 2019;Nikolic, Tolinacki, Fira, Golic, & Topisirovic, 2009;Vukotic et al, 2015). This correlated with improved skeletal differentiation in suddenly early-weaned larval fish (Ljubobratovic et al, 2020).…”

Antimicrobial Activity, Genetic Relatedness, and Safety Assessment of Potential Probiotic Lactic Acid Bacteria Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis) Used as Live Feed in Fish Larviculture

“…Moreover, the use of Lc. paracasei strains has been associated with improved fish feed utilization and nutrient bioavailability [62].…”