Solid supported lipid bilayers: From biophysical studies to sensor design

Castellana, Edward T.; Cremer, Paul S.

doi:10.1016/j.surfrep.2006.06.001

Cited by 1,002 publications

(1,042 citation statements)

References 158 publications

(260 reference statements)

Supporting

Mentioning

1,027

Contrasting

Unclassified

Order By: Relevance

“…During the past two decades, SLBs have been decorated with a variety of membrane-associated molecules, coated on various solid supports and coupled with micro-fluidic and microarray techniques. 4,5 These advances have enabled the development of SLB-based membrane-mimicking systems for sensing membrane reactions, studying intercellular signaling and separating membrane species. [6][7][8][9] To simulate and monitor various cell membrane-based processes, the lateral heterogeneity in a subcellular dimension should be reproducibly created and the molecular interaction should be read out with high reproducibility and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

View full text Add to dashboard Cite

Cell membranes contain a variety of lipids and functional proteins that offer active platforms that organize the membrane components into functional assemblies and perform biologically important reactions. The dynamic and complex nature of the membranes makes them attractive materials, but at the same time, researchers report substantial experimental uncertainty in controlling the membrane reactions and extracting valuable information. The fascinating new structures and properties of nanomaterials could be utilized in addressing aforementioned issues, and the design and synthesis of lipid-nanostructure hybrids could be beneficial to the research areas in lipid-membrane biotechnology and nanobiotechnology. These hybrid structures possess dimensions that are comparable to that of biological molecules and structures and physicochemical properties that arise from both lipids and nanomaterials. Therefore, lipid-nanostructure hybrids offer additional options for control of the synthesized structures, provide new insight in understanding nanostructures and biological systems and allow the mimicking of functional subcellular membrane components and monitoring of the membrane-associated reactions in a highly sensitive and controllable manner. In this review, we present recent advances in the synthesis of various lipid-nanostructure hybrids and the application of these structures in biotechnology and nanotechnology. We further describe the scientific and practical applications of lipid-nanostructure hybrids for detecting membrane-targeting molecules, interfacing nanostructures with live cells and creating membrane-mimicking platforms to investigate various intercellular processes.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

View full text Add to dashboard Cite

show abstract

“…Numerous investigations have been driven on supported or tethered lipid bilayers because these systems, which can self-assemble due to their amphiphilic nature, provide a natural environment to membrane proteins immobilization [1]. To date, lipid bilayers [2] also including ion-channels [3], have been implemented in a field-effect transistor (FET) structure for pH and ionic detection in general.…”

Section: Introductionmentioning

confidence: 99%

Phospholipid film in electrolyte-gated organic field-effect transistors

Cotrone

Ambrico

Toss

et al. 2012

Organic Electronics

View full text Add to dashboard Cite

A totally innovative electrolyte-gated field effect transistor, embedding a phospholipid film at the interface between the organic semiconductor and the gating solution, is described. The electronic properties of OFETs including a phospholipid film are studied in both pure water and in an electrolyte solution and compared to those of an OFET with the organic semiconductor directly in contact with the gating solution. In addition, to investigate the role of the lipid layers in the charge polarization process and quantify the field-effect mobility, impedance spectroscopy was employed. The results indicate that the integration of the biological film minimizes the penetration of ions into the organic semiconductor thus leading to a capacitive operational mode as opposed to an electrochemical one. The OFETs operate at low voltages with a field-effect mobility in the 10 -3 cm 2 V -1 s -1 range and an on/off current ratio of 10 3 . This achievement opens perspectives to the development of FET biosensors potentially capable to operate in direct contact with physiological fluids.2

show abstract

“…[59][60][61] Moreover, various ideas for forming spatially addressed bilayer assays have been pursued over the past decade. [19][20][21][25][26][27][28]62 A key innovation of the present work is to combine spatially addressed arraying technology with microfluidics strategies.…”

Section: Discussionmentioning

confidence: 99%

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

2008

Self Cite

View full text Add to dashboard Cite

A method has been designed for patterning supported phospholipid bilayers (SLBs) on planar substrates and inside microfluidic channels. To do this, bovine serum albumin (BSA) monolayers were formed via adsorption at the liquid/solid interface. Next, this interfacial protein film was selectively patterned by using deep UV lithography. Subsequently, SLBs could be deposited in the patterned locations by vesicle fusion. By cycling through this process several times, spatially addressed bilayer arrays could be formed with intervening protein molecules serving as twodimensional corrals. By employing this method, phospholipid bilayers containing various concentrations of ganglioside GM1 were addressed along the length of individual microfluidic channels. Therefore, the binding of GM1 with pentameric cholera toxin B (CTB) subunits could be probed. A seven-channel microfluidic device was fabricated for this purpose. Each channel was simultaneously patterned with four chemically distinct SLBs containing 0, 0.2, 0.5, and 2.0 mol % GM1, respectively. Varying concentrations of CTB were then introduced into each of the channels. With the use of total internal reflection fluorescence microscopy, it was possible to simultaneously abstract multiple equilibrium dissociation constants as a function of ligand density for the CTB-GM1 system in a single shot.Multivalent ligand-receptor interactions are ubiquitous on cell surfaces. They have a wide variety of consequences including the modulation of equilibrium dissociation constants, high receptor selectivity, and receptor clustering. 1 Multivalency can also play a direct role in signal transduction processes. 2 Examination of the underlying thermodynamics of ligandreceptor binding may lead to a greater understanding of its biological role and could provide insight into biomedical applications involving inhibitory drug design. 1,3,4 Unfortunately, high-throughput, low-protein consumption assays are not presently well enough developed in this field to afford rapid, systematic studies of binding data at two-dimensionally fluid bilayer interfaces. 5 In previous work it has been demonstrated that microfluidic devices can be designed for measuring binding affinities in multivalent systems. [5][6][7][8][9][10][11][12][13][14] In our previous setup, ligands were incorporated into supported lipid bilayers (SLBs) coated on the walls and floors of polydimethylsiloxane/glass microchannels. Linear arrays of channels were then monitored by total internal reflection fluorescence microscopy (TIRFM) 15 to obtain equilibrium dissociation constants in one-shot assays. This could be done by using the same surface chemistry in each microchannel while varying the solution concentration of the aqueous proteins. Such a setup made these assays relatively rapid, afforded high accuracy, and used only a few microliters of protein solution. Nevertheless, such platforms could be markedly improved by measuring multiple binding constants with various membrane chemistries simultaneously. This could be achie...

show abstract

Solid supported lipid bilayers: From biophysical studies to sensor design

Cited by 1,002 publications

References 158 publications

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Phospholipid film in electrolyte-gated organic field-effect transistors

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

Contact Info

Product

Resources

About