Solid supports for extraction and preconcentration of proteins and peptides in microfluidic devices: A review

Dziomba, Szymon; Araya-Farias, Monica; Smadja, Claire; Taverna, Myriam; Carbonnier, Benjamin; Tran, Nguyet Thuy

doi:10.1016/j.aca.2016.12.017

Cited by 33 publications

(21 citation statements)

References 215 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…. Special reviews and papers deal with the particular sample clean‐up techniques, such as SPE , solid‐phase microextraction (SPME) , electromembrane extraction , selective electromembrane extraction based on isoelectric point , magneto‐immuno capture , and on‐line electrodriven sample‐stacking techniques . The latter electrodriven procedures include field amplified sample stacking , field enhanced or field‐amplified sample injection (FESI or FASI) ; large volume sample stacking (LVSS) , transient‐ITP (t‐ITP) , multiple capillary ITP (CITP) with repetitive hydrodynamic injection , dynamic pH junction , electrokinetic supercharging , and sweeping .…”

Section: Sample Preparationmentioning

confidence: 99%

See 1 more Smart Citation

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

View full text Add to dashboard Cite

The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

show abstract

Section: Sample Preparationmentioning

confidence: 99%

“…The classical off‐line formats of SPE are nowadays being substituted by their on‐line modes. Both preconcentration and preseparation are achieved by a solid‐phase packed or monolithic sorbent at the inlet capillary end. Approaches employed for increasing sensitivity in chiral CE methods are presented in recent review .…”

Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…Polymer monolithic materials are usually easy to be prepared in capillary tubes with the characteristics of porous structure, rapid mass transfer, and good biocompatibility . Because of these advantages, the development and application of them have received substantial attention in separation science . In recent years, PMME has been applied in the field of bio‐separation such as proteins, peptides, and peptides with post‐translational modifications (PTMs) .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selective enrichment of glycopeptides based on copper tetra(N-carbonylacrylic) aminephthalocyanine and iminodiacetic acid functionalized polymer monolith

Zhang

Jiang

et al. 2019

J. Sep. Sci.

View full text Add to dashboard Cite

Efficient separation and enrichment of low‐abundance glycopeptides from complex biological samples is the key to the discovery of disease biomarkers. In this work, a new material was prepared by coating copper tetra(N‐carbonylacrylic) aminephthalocyanine and iminodiacetic acid onto poly(glycidyl methacrylate‐pentaerythritol triacrylate) monolith. The monolith was applied to polymer monolithic microextraction for specific capture of glycopeptides coupled with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. The developed monolith exhibited satisfactory efficiency for glycopeptide enrichment with high selectivity and detection sensitivity. When the tryptic digest of immunoglobulin G was used as the sample, total 24 glycopeptides were identified and the detection limit was determined as 5 fmol. When the approach was applied to the analysis of glycopeptides in the mixture of bovine serum albumin and immunoglobulin G (100:1, m/m) digests, 16 glycopeptides could still be observed. Moreover, the monolith was successfully applied to the selective enrichment of glycopeptides from human serum digests, exhibiting great practicability in identifying low‐abundance glycopeptides in complex biological samples.

show abstract

“…Sample preconcentration plays a key role in modern analytical methodology, which affects the accuracy and precision of the final results . Liquid–liquid extraction (LLE) is a widely used sample preconcentration technique.…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid‐based headspace in‐tube liquid‐phase microextraction coupled with CE for sensitive detection of phenols

Yue

Lin

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

An ionic liquid-based headspace in-tube liquid-phase microextraction (IL-HS-ITLPME) in-line coupled with capillary electrophoresis (CE) is proposed. The method is capable of quantifying trace amounts of phenols in environmental water samples. In the newly developed method, simply by placing a capillary injected with IL in the HS above the aqueous sample, volatile phenols were extracted into the IL acceptor phase in the capillary. After extraction, electrophoresis of the phenols in the capillary was carried out. Extraction parameters such as the extraction time, extraction temperature, ionic strength, volume of the sample solution and IL types were systematically investigated. Under the optimized conditions, enrichment factors for four phenols were from 1510 to 1985. The proposed method provided a good linearity, low limits of detection (below 5.0 ng mL ), and good repeatability of the extractions (RSDs below 6.7%, n = 6). This method was then utilized to analyze two real environmental samples of Xiaoxi Lake and tap water, obtaining acceptable recoveries and precisions. Compared with the usual HS-ITLPME for CE, IL-HS-ITLPME-CE is a simple, low-cost, fast and environmentally friendly pre-concentration technique. This article is protected by copyright. All rights reserved.

show abstract

Solid supports for extraction and preconcentration of proteins and peptides in microfluidic devices: A review

Cited by 33 publications

References 215 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Selective enrichment of glycopeptides based on copper tetra(N-carbonylacrylic) aminephthalocyanine and iminodiacetic acid functionalized polymer monolith

Ionic liquid‐based headspace in‐tube liquid‐phase microextraction coupled with CE for sensitive detection of phenols

Contact Info

Product

Resources

About