Solidification of the Blends of Fully Hydrogenated Coconut Oil and Non-hydrogenated Coconut Oil

Dhaygude, Vinod; Soós, Anita; Somogyi, László

doi:10.3311/ppch.9638

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Living organisms incorporate FAs into a wide variety of complex lipids, including phosphoglycerolipids and sphingolipids forming the bilayers in the cellular membranes, and also triglycerides for a fuel storage [1]. The depot fat is stored in the adipocytes in form of lipid droplets and its triglyceride molecules contain endogenously synthetized FAs as well as FAs ingested with natural or hydrogenated plant oils [2,3] or animal fat [4]. The optimal physical properties of biological membranes and fat depots requires a balanced utilization of both saturated and unsaturated FAs [5].…”

Section: Introductionmentioning

confidence: 99%

Application of Gas Chromatography – Flame Ionization Detection to Study Cellular Incorporation of Dietary Trans Fatty Acids of Medical Importance

Somogyi

Mátyási

Gór-Nagy

et al. 2021

Period. Polytech. Chem. Eng.

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Putative health effects of dietary trans fatty acids (TFAs) receive a growing attention; while very little is known about the metabolism of these special food components. In vitro studies carried out in cultured cells provide an efficient and standardizable approach to follow the metabolic fate of TFAs, but it requires suitable techniques for the quantitative measurement of FAs in cell samples. Here, the development and validation of a simple and reliable method for the quantification of a group of relevant FAs by gas chromatography and flame ionization detection is presented. Sample preparation used a fast one-step and chloroform-free process for simultaneous extraction and esterification, and chromatographic separation was achieved in 25 min using a Zebron ZB-88 capillary column. A linear calibration (of R2 >0.99) was obtained in the concentration range of 1-200 µg/mL for each FA. Recovery rate was 82 % for samples of non-esterified FAs and >95 % for complex lipids, such as ceramides, diglycerides and triglycerides. The LOD and LOQ were below 0.5 µg/mL, and a robust method precision was achieved (RSD % was below 6 % for each lipid classes). The present method was also tested on a cultured cell line with or without FA treatment at close to physiological concentration, and the observed changes in the metabolite concentration levels revealed characteristic differences between the metabolism of cis and trans unsaturated FAs.

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Section: Introductionmentioning

confidence: 99%

Application of Gas Chromatography – Flame Ionization Detection to Study Cellular Incorporation of Dietary Trans Fatty Acids of Medical Importance

Somogyi

Mátyási

Gór-Nagy

et al. 2021

Period. Polytech. Chem. Eng.

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“…Depot fat stored in the adipocytes contains fatty acids (FAs) ingested with animal fat [1] and with natural or hydrogenated plant oils [2,3] as well as FAs synthesized endogenously in the fed state. As obesity and related diseases, such as cardiovascular diseases, insulin resistance and nonalcoholic fatty liver disease have become epidemic, there is a strong emphasis on investigating their pathology.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantitative Determination of Different Ceramide and Diacylglycerol Species in Cultured Cells by Using Liquid Chromatography–Electrospray Tandem Mass Spectrometry

Somogyi

Donkó

Sarnyai

et al. 2020

Period. Polytech. Chem. Eng.

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A sensitive, reproducible reverse-phased high performance liquid chromatography electrospray tandem mass spectrometry (HPLC-ESI-MS/MS) method with simple sample preparation was developed for the simultaneous determination of a wide range of ceramides, diacylglycerols (DAGs) in cultured cells. Chromatographic separation of the compounds was achieved in a 14-minute run using a C8 column with a gradient elution by methanol and 10 mM ammonium acetate buffer as mobile phase at a flow rate of 0.5 ml/min. Various ceramides, DAGs were detected with a triple quadrupol system in multiple reaction monitoring mode, which is based on a soft positive electrospray ionization. The usual sample preparation process was shortened by the application of pure methanol for the extraction instead of the widely used methanol/chloroform mixture. C17:0 ceramide which does not occur in the cell samples, was used as an internal standard. The sample preparation process was optimized and the methodology was tested on a human hepatocarcinoma cell culture. Our results clearly showed accumulation of some ceramides and DAGs in the cells treated with BSA-conjugated palmitate for 8 hours. Since both ceramides and DAGs are important lipid intermediates and signal messengers, alteration in their cellular levels have major impact on cell functions, and thus our novel analytic method can be widely used in lipotoxicity research. The presented technique can be further developed to measure other intermediates of ceramide synthesis and other derivatives of DAGs as well.

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Solidification of the Blends of Fully Hydrogenated Coconut Oil and Non-hydrogenated Coconut Oil

Abstract: Abstract

Cited by 2 publications

References 16 publications

Application of Gas Chromatography – Flame Ionization Detection to Study Cellular Incorporation of Dietary Trans Fatty Acids of Medical Importance

Application of Gas Chromatography – Flame Ionization Detection to Study Cellular Incorporation of Dietary Trans Fatty Acids of Medical Importance

Simultaneous Quantitative Determination of Different Ceramide and Diacylglycerol Species in Cultured Cells by Using Liquid Chromatography–Electrospray Tandem Mass Spectrometry

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