Solubilisation of model membrane by DDAO surfactant – partitioning, permeabilisation and liposome-micelle transition

Želinská, K.; Gallová, Jana; Huláková, Silvia; Uhrı́ková, Daniela; Ivankov, Oleksandr I.

doi:10.4149/gpb_2019056

Cited by 2 publications

(4 citation statements)

References 65 publications

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“…The parameters of the model are scale factor Γ m , bilayer thickness d L (discussed in the next paragraph), number of layers N L , and repeat distance d (SANS analysis, eqs S5–S7). Our previous studies confirmed the suitability of the paracrystal lamellar model for lipidadditive oligolamellar vesicles (OLV). , …”

Section: Resultssupporting

confidence: 84%

“…These values correspond to the bilayer thickness reported d L = 3.76 nm of bovine lung surfactant in fluid state, and d L = 4.13 ± 0.02 nm derived for Curosurf at 40 °C by Oseliero Filho et al For protein-free MS, d L = 3.65 ± 0.02 nm was obtained from the best fit of the SANS data at 37 °C using the same model (Figure S11). The model was previously tested and, for comparison, we have obtained d L = 3.61–3.87 (±0.02) nm for ULV from DPPC and DOPC in the fluid state, while d L = 4.20 ± 0.02 nm for DPPC vesicles in the gel state. , …”

Section: Resultsmentioning

confidence: 93%

“…The model was previously tested and, for comparison, we have obtained d L = 3.61−3.87 (±0.02) nm for ULV from DPPC and DOPC in the fluid state, while d L = 4.20 ± 0.02 nm for DPPC vesicles in the gel state. 91,92 Detailed inspection of the SANS curves of the PxB-PSUR and PxB-MS mixtures (Figures 4A, S11, and S12) revealed that the addition of PxB induces the aggregation of EPS unilamellar vesicles. The effect is proved by the Kratky−Porod plot, 93,94 ln(I.q 2 ) vs. q 2 , where I(q) is the scattered intensity, and is shown for selected data in the inset in Figure 4A.…”

Section: Role Of Electrostatic Interactions (Saxs and Sans)mentioning

confidence: 99%

“…Our previous studies confirmed the suitability of the paracrystal lamellar model for lipid�additive oligolamellar vesicles (OLV). 91,95 PxB is associated with negatively charged EPS vesicles (PSUR and MS) as a result of electrostatic forces, resulting in the formation of OLV. The fitting output N L , characterizing the number of interacting bilayers, is described in the lamellar paracrystal model by Z N (q) in the formula eq S5 and its meaning by eq S6.…”

Section: Role Of Electrostatic Interactions (Saxs and Sans)mentioning

confidence: 99%

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Polymyxin B-Enriched Exogenous Lung Surfactant: Thermodynamics and Structure

Královič-Kanjaková,

Asi Shirazi,

Hubčík

et al. 2024

Langmuir

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The use of an exogenous pulmonary surfactant (EPS) to deliver other relevant drugs to the lungs is a promising strategy for combined therapy. We evaluated the interaction of polymyxin B (PxB) with a clinically used EPS, the poractant alfa Curosurf (PSUR). The effect of PxB on the protein-free model system (MS) composed of four phospholipids (diC16:0PC/16:0–18:1PC/16:0–18:2PC/16:0–18:1PG) was examined in parallel to distinguish the specificity of the composition of PSUR. We used several experimental techniques (differential scanning calorimetry, small- and wide-angle X-ray scattering, small-angle neutron scattering, fluorescence spectroscopy, and electrophoretic light scattering) to characterize the binding of PxB to both EPS. Electrostatic interactions PxB–EPS are dominant. The results obtained support the concept of cationic PxB molecules lying on the surface of the PSUR bilayer, strengthening the multilamellar structure of PSUR as derived from SAXS and SANS. A protein-free MS mimics a natural EPS well but was found to be less resistant to penetration of PxB into the lipid bilayer. PxB does not affect the gel-to-fluid phase transition temperature, T m, of PSUR, while T m increased by ∼+ 2 °C in MS. The decrease of the thickness of the lipid bilayer (d L) of PSUR upon PxB binding is negligible. The hydrophobic tail of the PxB molecule does not penetrate the bilayer as derived from SANS data analysis and changes in lateral pressure monitored by excimer fluorescence at two depths of the hydrophobic region of the bilayer. Changes in d L of protein-free MS show a biphasic dependence on the adsorbed amount of PxB with a minimum close to the point of electroneutrality of the mixture. Our results do not discourage the concept of a combined treatment with PxB-enriched Curosurf. However, the amount of PxB must be carefully assessed (less than 5 wt % relative to the mass of the surfactant) to avoid inversion of the surface charge of the membrane.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 93%

Section: Role Of Electrostatic Interactions (Saxs and Sans)mentioning

confidence: 99%

Section: Role Of Electrostatic Interactions (Saxs and Sans)mentioning

confidence: 99%

See 2 more Smart Citations

Polymyxin B-Enriched Exogenous Lung Surfactant: Thermodynamics and Structure

Královič-Kanjaková,

Asi Shirazi,

Hubčík

et al. 2024

Langmuir

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Study of the solubilisation process of bacterial model membranes induced by DDAO

Želinská

Gallová

2021

European Pharmaceutical Journal

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Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a second model membrane was enriched with tetraoleoylcardiolipin (TOCL) with a composition POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol. Solubilisation of these model membranes was studied by static light scattering (nephelometry). Effective ratio Re (the amount of DDAO integrated into the bilayer to the amount of lipid) at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO was calculated – in case of the POPE-POPG membrane, Kp = 5,300 ± 400, for the POPE-POPG-TOCL membrane, Kp = 6,500 ± 500.

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Solubilisation of model membrane by DDAO surfactant – partitioning, permeabilisation and liposome-micelle transition

Abstract: Dedicated to prof. P. Balgavý on the occasion of his 75th birthday.

Cited by 2 publications

References 65 publications

Polymyxin B-Enriched Exogenous Lung Surfactant: Thermodynamics and Structure

Polymyxin B-Enriched Exogenous Lung Surfactant: Thermodynamics and Structure

Study of the solubilisation process of bacterial model membranes induced by DDAO

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