Solubility-based Separation and Purification of Long-Chain Chitin Oligosaccharides with an Organic-Water Mixed Solvent

Abstract

A simple and rapid method for separation and purification of chitin oligosaccharides, (GlcNAc)n, with n ≥ 5 is presented. A commercially available chitin oligosaccharides sample, consisting of (GlcNAc)n with n = 1 -7, was used as the starting material. Ten milligrams of the material was mixed with 100 μL of the 1 mol/L HCl. All the (GlcNAc)n species were dissolved in the aqueous medium. The aqueous solution was mixed with 900 μL of EtOH; the mixture was centrifuged, and the supernatant was removed to obtain a precipitate. The precipitate was found to consist mainly of (GlcNAc)n with n ≥ 5, indicating the significant difference in solubility between the short-chain (GlcNAc)n species with n ≤ 3 and the longer ones. By the repetition of the operations, a high purity long-chain (GlcNAc)n sample with n ≥ 5 could be prepared successfully. Since the long-chain (GlcNAc)n species are known to have excellent elicitor activity, this sample would be useful in the study of plant pathology, as well as chitin and chitosan chemistry.

Three‐Enzyme Phosphorylase Cascade for Integrated Production of Short‐Chain Cellodextrins

“…The yield of G5 and G6 was ≈80% whereas that of G3 and G4 was just ≈50% (Figure B). These results are consistent with the literature on organic solvents for oligosaccharide purification …”

Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria

“…As shown previously, 29 , 45 COS precipitation with acetone (by 9 times the volume of sample) was used to isolate COS from the products of the yeast fermentation, in particular ethanol, glycerol, and acetic acid. 46 The COS were precipitated quantitatively, and redissolution of the solid pellet in the original volume of water gave a concentration of 92 g/L.…”

Chitosan oligosaccharides exert neuroprotective effectsviamodulating the PI3K/Akt/Bcl-2 pathway in a Parkinsonian model

“…Solubility-based separation and purification enriched the content of COS with DPs < 6 in an alcohol-water (4 : 1, v/v) mixed solvent to obtain sample2. 42 The TLC analysis (Fig. 2B) of sample2 showed that the content of COS with DP < 6 was significantly increased compared with that of sample1.…”