Solubility of Rosmarinic Acid in Supercritical Carbon Dioxide Extraction from Orthosiphon stamineus Leaves

Aziz, Ahmad Hazim Abdul; Idrus, Nor Faadila Mohd; Putra, Nicky Rahmana; Awang, Mohd Azrie; Idham, Zuhaili; Mamat, Hasmadi; Yunus, Mohd Azizi Che

doi:10.3390/chemengineering6040059

Cited by 17 publications

(10 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The preparation of RA systems with CDs resulted in an efficient increase in solubility; the most extraordinary outcomes were obtained for RA–HP-γ-CD s.e., where there was a 33-fold increase to 194.354 ± 1.847 mg/mL. Hazim et al designed a study where the supercritical carbon dioxide extraction of Orthosiphon stamineus leaves with the addition of ethanol was performed, and the optimization of the process to correlate the solubility of RA in supercritical carbon dioxide [ 54 ]. The greatest solubility was obtained in 80 °C and 10 MPa with 2.004 mg of RA/L solvent.…”

Section: Discussionmentioning

confidence: 99%

Cyclodextrin Derivatives as Promising Solubilizers to Enhance the Biological Activity of Rosmarinic Acid

et al. 2022

View full text Add to dashboard Cite

Rosmarinic acid (RA) is a natural antioxidant with neuroprotective properties; however, its preventive and therapeutic use is limited due to its slight solubility and poor permeability. This study aimed to improve RA physicochemical properties by systems formation with cyclodextrins (CDs): hydroxypropyl-α-CD (HP-α-CD), HP-β-CD, and HP-γ-CD, which were prepared by the solvent evaporation (s.e.) method. The interactions between components were determined by X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC) and Fourier Transform infrared spectroscopy (FTIR). The sites of interaction between RA and CDs were suggested as a result of in silico studies focused on assessing the interaction between molecules. The impact of amorphous systems formation on water solubility, dissolution rate, gastrointestinal (GIT) permeability, and biological activity was studied. RA solubility was increased from 5.869 mg/mL to 113.027 mg/mL, 179.840 mg/mL, and 194.354 mg/mL by systems formation with HP-α-CD, HP-β-CD, and HP-γ-CD, respectively. During apparent solubility studies, the systems provided an acceleration of RA dissolution. Poor RA GIT permeability at pH 4.5 and 5.8, determined by parallel artificial membrane permeability assay (PAMPA system), was increased; RA–HP-γ-CD s.e. indicated the greatest improvement (at pH 4.5 from Papp 6.901 × 10−7 cm/s to 1.085 × 10−6 cm/s and at pH 5.8 from 5.019 × 10−7 cm/s to 9.680 × 10−7 cm/s). Antioxidant activity, which was determined by DPPH, ABTS, CUPRAC, and FRAP methods, was ameliorated by systems; the greatest results were obtained for RA–HP-γ-CD s.e. The inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) was increased from 36.876% for AChE and 13.68% for BChE to a maximum inhibition of the enzyme (plateau), and enabled reaching IC50 values for both enzymes by all systems. CDs are efficient excipients for improving RA physicochemical and biological properties. HP-γ-CD was the greatest one with potential for future food or dietary supplement applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Cyclodextrin Derivatives as Promising Solubilizers to Enhance the Biological Activity of Rosmarinic Acid

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Pressure is one of the important elements governing the ScCO 2 process due to its influence on the solubility of compounds and diffusivity of the solvent [ 20 , 62 , 72 ]. Utilizing a back pressure regulator that maintains the desired pressure of ScCO 2 may be used to conduct pressure control in the ScCO 2 approach.…”

Section: Parameter Effects On Scco 2 Extractionmentioning

confidence: 99%

“…However, raising the temperature improves the solubility and extraction yield of the target compounds by increasing their vapor pressure. Consequently, this may result in the crossing of the isotherms, a process known as retrogradation in which higher-temperature conditions result in low yields and low temperatures result in a higher yield [ 11 , 20 , 25 ]. The inversion of yield isotherms is a result of these competing influences on the overall extraction yield.…”

Section: Parameter Effects On Scco 2 Extractionmentioning

confidence: 99%

“…This method is a novel approach for optimizing the extraction of chemicals of interest, especially for anthocyanins since it is a non-hazardous and eco-friendly solvent. Mohd-Nasir, et al [ 19 ] and Abdul Aziz, et al [ 20 ] reported that this technology has many benefits over traditional extraction techniques, including a reduction in extraction time, an improvement in extract quality, reduced extraction agent costs, and an eco-friendly operation. This technique is rapidly gaining acceptance as an alternative to solid sample extraction [ 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insight into Green Extraction for Roselle as a Source of Natural Red Pigments: A Review

et al. 2023

Self Cite

View full text Add to dashboard Cite

Roselle (Hibiscus sabdariffa L.) is a source of anthocyanins as red pigments that is extensively farmed in tropical and subtropical regions, including Indonesia, Malaysia, China, Thailand, Egypt, Mexico, and West India. The roselle plant contains a variety of nutrients, including anthocyanins, organic acids, pectin, etc. Due to the toxicity and combustibility of the solvents, traditional extraction methods for these compounds are restricted. Obtaining pure extracts is typically a lengthy procedure requiring many processes. Supercritical carbon dioxide (ScCO2) extraction as a green technology is rapidly improving and extending its application domains. The advantages of this method are zero waste production, quicker extraction times, and reduced solvent consumption. The ScCO2 extraction of natural pigments has great promise in food, pharmaceuticals, cosmetics, and textiles, among other uses. The ScCO2 technique for natural pigments may also be advantageous in a variety of other contexts. Due to their minimal environmental risk, the high-quality red pigments of roselle rich in anthocyanins extracted using ScCO2 extraction have a high sustainability potential. Therefore, the objective of this review is to increase knowledge related to the natural colorant of roselle as a substitute for chemically manufactured colorants using ScCO2 as a green method. This article covers ScCO2 extraction, particularly as it relates to the optimization of pigments that promote health. This article focuses on the high extraction efficiency of ScCO2 extraction. Natural colorants extracted via ScCO2 are regarded as safe compounds, especially for human consumption, such as novel functional food additives and textile and pharmaceutical colors.

show abstract

“…Therefore, there is an urgent need for a more advanced new technology with less environmental pollution and less damage to functional components. The supercritical carbon dioxide extraction [12][13][14][15] is a green, efficient, and highly selective extraction method, and its extraction at near room temperature can effectively protect the functional components from being destroyed. The principle of the method [12][13][14][15] is to use the varying dissolving effects of natural products in supercritical carbon dioxide to separate these products.…”

Section: Introductionmentioning

confidence: 99%

Supercritical Carbon Dioxide Extraction Process for Hibiscus taiwanensis S. Y. Hu and Tyrosinase Inhibitory Activity of Its Extract

Yang

Pan

2022

Processes

View full text Add to dashboard Cite

In this study, the bioactive components in the stem of Hibiscus taiwanensis S. Y. Hu were extracted by supercritical carbon dioxide (SC-CO2), and the inhibition activity of the extract tyrosinase was analyzed. When the particle size of the powder was controlled to be 50 mesh, the effects of temperatures (40, 45, 50, 55, and 60 °C), pressures (15, 20, 25, 30, and 35 MPa), concentrations of entrainment agent (ethanol) (60, 80, and 95%) and CO2 flow rates (80, 100, 120, and 140 L/min) on the extraction rate, respectively, were studied with the single-variable method. The optimal parameters included an extraction temperature of 55 °C, an extraction pressure of 30 MPa, 80% ethanol as the entrainment agent, and the CO2 flow rate of 100 L/min. Under the optimal conditions, the extraction rate of Hibiscus taiwanensis S. Y. Hu reached more than 0.5% within 2 h. At the same time, the chemical compositions of the extract were investigated by using high-resolution liquid chromatography-mass spectrometry (LC-MS) equipped with a linear ion trap-electrostatic field orbital trap. In addition, the related activity of extract in the stem Hibiscus taiwanensis S. Y. Hu was evaluated by tyrosinase inhibition experiments. The results showed that the inhibition rate of the extract with a concentration of 0.25 mg/mL to tyrosinase reached 35.60%, indicating that the extract by SC-CO2 had the potential to be used for whitening active raw materials.

show abstract

Solubility of Rosmarinic Acid in Supercritical Carbon Dioxide Extraction from Orthosiphon stamineus Leaves

Cited by 17 publications

References 58 publications

Cyclodextrin Derivatives as Promising Solubilizers to Enhance the Biological Activity of Rosmarinic Acid

Cyclodextrin Derivatives as Promising Solubilizers to Enhance the Biological Activity of Rosmarinic Acid

Insight into Green Extraction for Roselle as a Source of Natural Red Pigments: A Review

Supercritical Carbon Dioxide Extraction Process for Hibiscus taiwanensis S. Y. Hu and Tyrosinase Inhibitory Activity of Its Extract

Contact Info

Product

Resources

About