Solubilization and stabilization of bacteriophage MS2 in organic solvents

Johnson, Harvey R.; Hooker, Jacob M.; Francis, Matthew B.; Clark, Douglas S.

doi:10.1002/bit.21245

Cited by 36 publications

(30 citation statements)

References 31 publications

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“…The left part of the scale represents short-term treatments, which typically take place in aerosol systems, while the right part features much longer thermal exposures occurring in liquid suspensions. In this figure, we plotted the actual experimental data obtained in the present study with aerosolized MS2 virions (circles) as well as the data reported by Stonehouse and Stockley (1993) and Johnson et al (2007) from their tests on the inactivation of MS2 bacteriophage in liquid (triangle and diamond).…”

Section: Discussionmentioning

confidence: 99%

“…Johnson et al (2007) compared the recovery of the capsid proteins after heating for 2 h using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reported that no capsids were recovered at temperatures above 70…”

Section: Discussionmentioning

confidence: 99%

“…While some methods achieve this goal by reducing the total aerosol concentration, others specifically target viable bioaerosol particles. Heat treatment has been found efficient for inactivating viable bacteria (Northrop and Slepecky 1967;Setlow 1995;Setlow and Setlow 1995;Stumbo 1973) and viruses (Johnson et al 2007;Stonehouse and Stockley 1993). However, most of the previous studies have been primarily motivated by "non-aerosol" applications, such as the sterilization of food products, water purification, and surface decontamination.…”

Section: Introductionmentioning

confidence: 99%

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Inactivation of Aerosolized Viruses in Continuous Air Flow with Axial Heating

Grinshpun

Adhikari

et al. 2010

Aerosol Science and Technology

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Thermal inactivation of viruses has been studied in relevance to food sterilization, water purification, and other "non-aerosol" applications, in which heat treatment is applied for a relatively long time. No data are available on the inactivation of airborne viruses exposed to dry heat for a short time, although this is relevant to biodefense and indoor air quality control. In this study, we investigated inactivation of aerosolized MS2 viruses in a continuous air flow chamber with axial heating resulted from exposures during ∼0.1-1 s. For an airborne virion, the characteristic exposure temperature, T e , was defined utilizing the air temperature profiles in the chamber. The tests were conducted at two air flow rates, Q, which allowed for establishing different thermal flow regimes and exposure time intervals. The experimentally determined inactivation factor, IF, was subjected to correction to account for the temperature profiles. At T e up to ∼90• C (Q = 18 L/min) and up to ∼140• C (Q = 36 L/min), the loss of viral infectivity was relatively modest (≤ 10). However, IF increased exponentially as T e rose from ∼90• C to ∼160• C (for 18 L/min) or from ∼140 • C to ∼230• C (for 36 L/min). Under specific thermal exposure conditions (∼170• C and ∼250 • C, respectively), IF exceeded ∼2.4 × 10 4 (∼99.996% infectivity loss)-the maximum quantifiable in this study. The airborne MS2 virions exposed to hot air for <1 s were found to have survived much higher temperatures than those subjected to thermal treatment in liquid for minutes or hours. The findings are significant for establishing limitations of the heat-based bioaerosol control methods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inactivation of Aerosolized Viruses in Continuous Air Flow with Axial Heating

Grinshpun

Adhikari

et al. 2010

Aerosol Science and Technology

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“…In this figure, we plotted the actual experimental data obtained in the present study with aerosolized MS2 virions (red circles) as well as the data reported by Stonehouse and Stockley (1993) and Johnson et al (2007) In contrast, manifestation of the temperature-time dependence is anticipated when virions are exposed to heat over sub-second time intervals, which is sufficient for adopting the surrounding air temperature but may fall short of providing sufficient time for the expected lethal response. Thus, the airborne virions exposed to hot air for less than one second may survive much higher temperatures than those suspended in hot liquid media for minutes or hours.…”

Section: Air Tmentioning

confidence: 77%

Section: Chapter 3 Inactivation Of Aerosolized Viruses In Continuousmentioning

confidence: 99%

Inactivation of Aerosolized Biological Agents using Filled Nanocomposite Materials

Grinshpun¹,

Schoenitz²,

Dreizin³

et al. 2013

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE (DD-MM-YYYY)2. REPORT TYPE 3. DATES COVERED (From -To) Approved for public release; distribution is unlimited.In this multi-institutional grant, a new method for inactivating aerosolized biological agents was developed utilizing a new class of energetic materials: filled nanocomposite materials. The implemented approach enabled a controllable release of iodine-based oxidizing species in the combustion environment to inactivate viable airborne bio-agents, such as stress-resistant bacterial spores and viruses. Composites with adjustable Al/I2 ratios were produced. A state-of-the-art experimental facility was developed for studying how novel energetic formulations and their combustion products affect the viability of aerosolized spores and viruses during a short (< 1 s) exposure times. Controlled bioaerosol dispersal and sample collection protocols were developed and optimized. The dry-heat inactivation of aerosolized spores was quantified separately from chemical effects and linked to DNA repair mechanisms. It was concluded that the iodine-containing powder provided significantly more effective inactivation of airborne spores than non-iodinated powders. The results of this research help to better understand physical, physicochemical, and biological properties associated with inactivation of aerosolized bio-agents in combustion environments.Bioaerosol inactivation, spore, virus, bio-agent defeat, energetic material, iodine 1.000 000 x E -10 1.013 25 x E +2 1.000 000 x E +2 1.000 000 x E -28 1.054 350 x E +3 4.184 000 4.184 000 x E -2 3.700 000 x E +1 1.745 329 x E -2 t k = (t o f + 459.67)/1.8 1.602 19 x E -19 1.000 000 x E -7 1.000 000 x E -7 3.048 000 x E -1 1.355 818 3.785 412 x E -3 2.540 000 x E -2 1.000 000 x E +9 1.000 000 4.183 4.448 222 x E +3 6.894 757 x E +3 1.000 000 x E +2 1.000 000 x E -6 2.540 000 x E -5 1.609 344 x E +3 2.834 952 x E -2 4.448 222 1.129 848 x E -1 1.751 268 x E +2 4.788 026 x E -2 6.894 757 4.535 924 x E -1 4.214 011 x E -2 1.601 846 x E +1 1.000 000 x E -2 2.579 760 x E -4 1.000 000 x E -8 1.459 390 x E +1 1. ABSTRACTIn this multi-institutional grant, a new method for ...

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Selective and Responsive Nanoreactors

Renggli

Baumann

Langowska

et al. 2011

Adv Funct Materials

228

242

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Chemical reactions can be confi ned to nanoscale compartments by encapsulating catalysts in hollow nanoobjects. Such reaction compartments effectively become nanoreactors when substrate and product are exchanged between bulk solution and cavity. A key issue, thereby, is control of shell permeability. Nanoreactors exhibit selectivity and responsiveness if their shells discriminate among molecules and if access can be modulated by external triggers. Here, we review natural nanoreactors that include protein-based bacterial microcompartments, protein cages, and viruses. Artifi cial nanoreactors based on dendrimers, layer-by-layer capsules, and amphiphilic block copolymer polymersomes are also discussed. Selectivity in these nanoreactors is either due to intrinsic reactor-shell semipermeability or can be engineered using smart polymers to gate the reactors. Moreover, a rich repertoire of pores and channels are already provided in nature, e.g., in protein-based nanoreactors or in trans-membrane channel proteins. The latter can be reconstituted in polymersomes, resulting in gated vesicles. Nanoreactors hold promise for applications ranging from selective and size-constrained organic synthesis to biomedical advances (e.g., artifi cial organelles, biosensing) and as analytical tools to study reaction mechanisms.

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Solubilization and stabilization of bacteriophage MS2 in organic solvents

Cited by 36 publications

References 31 publications

Inactivation of Aerosolized Viruses in Continuous Air Flow with Axial Heating

Inactivation of Aerosolized Viruses in Continuous Air Flow with Axial Heating

Inactivation of Aerosolized Biological Agents using Filled Nanocomposite Materials

Selective and Responsive Nanoreactors

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