Solubilization of Molecular Forms of Lysosomal Acid Phosphatase of Solanum tuberosum L. Leaves during Infection by Phytophthora infestans (Mont.) de Bary

Pitt, D.

doi:10.1099/00221287-77-1-117

Cited by 15 publications

(1 citation statement)

References 13 publications

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“…One possibility which others have previously suggested (12,14) is the activation of host cell lysosomes. If this is the case, then resistance mediated by the M1-a gene is accomplished by activating the host cell's normal senescence or stress response.…”

Section: Cj7anges In Non-infected Host Cellsmentioning

confidence: 98%

The ultrastructure of M1-a-mediated resistance to powdery mildew infection in barley

Edwards¹

1975

Can. J. Bot.

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EDWARDS, H. H. 1975. The ultrastructure of MI-a-mediated resistance to powdery mildew infection in barley. Can. J. Bot. 53: 2589-2597. MI-a-mediated resistance in barley to invasion by the CR3 race of Etysiplle grarninis f. sp. hordei does not occur in every host cell with the same speed and severity. In some cells ultrastructural changes within the host cell as a result of resistance will occur within 24 h after inoculation, whereas in other cells these changes may take up to 72 h. In some cells the ultrastructural changes are so drastic that they give the appearance of a hypersensitive death of the host cell, whereas in other cells the changes are very slight. In any case, at the end of these changes the fungus ceases growth. The ultrastructural changes occur in penetrated host epidermal cells as well as non-infected adjacent epidermal and mesophyll cells.The following ultrastructural changes have been observed: (I) an electron-dense material which occurs either free in the vacuole or adhering to the tonoplast (the material is granular or in large clumps); (2) an increased electron density of the host cytoplasm and nucleus; (3) a breakdown of the tonoplast so that the cytoplasmic constituents become dispersed throughout the cell lumen; and (4) the deposition of papillar-like material in areas other than the penetration site. The first three changes take place within the host cell protoplasts andare directly attributable to the gene MI-a. These changes are typical of stressor incompatibility responses and thus MI-a appears to trigger a generalized incompatibility response in the presence of race CR3. The papillar-like material occurs outside the host cell protoplast in the same manner as the papilla and probably is not directly attributable to MI-a. EDWARDS, H. H. 1975. The ultrastructure of MI-[I-mediated resistance to powdery mildew infection in barley. Can. J. Bot. 53: 2589-2597. Chez I'orge, la resistance d'origine MI-a a I'invasion par la race CR3 d'Erysiphe grar~ritlis f. sp. 11ordei.ne s'effectue pas dans toutes les cellules avec la mtme vitesse et avec la mtme intensite. Chez certaines cellules, les changements ultrastructuraux dans la cellule-hbte, resultant de la resistance, ont lieu moins de 24 h apres I'inoculation, alors que dans les autres cellules ces changements peuvent prendre jusqu'i 72 h. Dans certaines cellules les changements ultrastructuraux sont tellement brutaux qu'ils prennent I'apparence d'une mortalit6 par hypersensibilite de la cellule-hbte, alors que dans d'autres cellules les changements sont minimes. Dans tous les cas, la croissance du champignon s'arrtte avec la fin de ces changements. Les changements ultrastructuraux se manifestent dans les cellules epidermiques que penetre le champignon, aussi'bien que dans les cellules adjacentes non infectkes de I'epiderme et du mksophylle.L'auteur a observe les changements ultrastructuraux suivants: (1) un materiel dense aux electtons qui se forme libre dans la vacuole ou accole au tonoplaste; ce materiel est granulaire ou sous forme de gros...

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Section: Cj7anges In Non-infected Host Cellsmentioning

confidence: 98%

The ultrastructure of M1-a-mediated resistance to powdery mildew infection in barley

Edwards¹

1975

Can. J. Bot.

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Sub-cellular Organization in Host-Parasite Interactions

Hislop¹,

Pitt²

1976

Physiological Plant Pathology

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Changes in activity of lysosomal ribonuclease following mechanical damage to leaves of Solanum tuberosum L.

Pitt

1975

Planta

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Differential centrifugation experiments showed that the 13-fold increase in total acid ribonuclease (RNase) activity arising during the 48 h following mechanical damage to potato leaves was associated, in about equal proportions, with the sedimentable and supernatant fluid fractions of cell homogenates. Density gradient ultracentrifugation techniques revealed that the majority of the particulate activity of RNase was located in the lysosomal fraction of leaf homogenates. Although [(14)C]leucine was incorporated into leaf lysosomes during incubation following mechanical damage the various molecular forms of RNase purified from the organelles, one of which had a specific lysosomal location, contained negligible radioisotope. That form of the enzyme which did incorporate [(14)C]leucine was one of those confined to the supernatant fluid fraction of leaves.In addition to the massive quantitative changes in RNase activity which occurred subsequent to mechanical damage, extensive qualitative changes occurred in the complement of molecular forms of lysosomal RNase. The possible origins of these changes are discussed.

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Solubilization of Molecular Forms of Lysosomal Acid Phosphatase of Solanum tuberosum L. Leaves during Infection by Phytophthora infestans (Mont.) de Bary

Cited by 15 publications

References 13 publications

The ultrastructure of M1-a-mediated resistance to powdery mildew infection in barley

The ultrastructure of M1-a-mediated resistance to powdery mildew infection in barley

Sub-cellular Organization in Host-Parasite Interactions

Changes in activity of lysosomal ribonuclease following mechanical damage to leaves of Solanum tuberosum L.

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