Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates

Malboobi, Mohammad Ali; Owlia, Parviz; Behbahani, Mandana; Sarokhani, Elaheh; Moradi, Sara; Yakhchali, Bagher; Deljou, Ali; Heravi, Kambiz Morabbi

doi:10.1007/s11274-009-0037-z

Cited by 110 publications

(70 citation statements)

References 19 publications

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“…Isolates PKJ25 and PKB27 were a concentration of NaCl upto 5% and a wide range of initial pH from 5 to 11 (optimum pH for PKJ25-5 and PKM11-5). Similar characteristics were described for bacteria belonging to genus Pseudomonas and species fluorescens (Sharma et al, 2007;Malviya and Singh, 2012) and putida (Pandey et al, 2006;Malboobi et al, 2009b) respectively. The result interpreted by Malboobi et al (2009a) in their investigation on PSB tolerance to extreme climates supported our findings.…”

Section: Resultssupporting

confidence: 75%

In vitro antagonistic activity of Pseudomonas spp. against Rhizoctonia soloni

Toppo¹,

Tiwari²

2015

Afr. J. Microbiol. Res.

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The present research work deals with the in-vitro study of antagonistic activity of Pseudomonas spp. against plantpathogenic fungi Rhizoctonia soloni. The bacterial strains were isolated from rhizospheric soil of Korea district of Chhattisgarh. A total of 28 bacterial cultures were isolated from 25 representative soil samples collected from five blocks of Korea district of Chhattisgarh, out of which four were identified as Pseudomonas spp. (PKS10-Pseudomonas syringae, PKM11-Pseudomonas syringae, PKJ25-Pseudomonas alcaligenes and PKB27-Pseudomonas alcaligenes) from Plant Pathology Division, IARI Delhi. These four Pseudomonas spp. were examined for their ability to antagonize R. soloni in in vitro plate assay by dual culture inoculation along with the standard check (Pmtcc-Pseudomonas isolate from IMTECH Chandigarh). The antagonistic activity was interpreted by restricted growth zone of the fungal pathogen in dual culture. The diameter of hyphal growth of the fungi in dual culture with isolates PKS10, PKM11, PKJ25, PKB27, Pmtcc and control were 3.82, 3.68, 2.73, 3.41, 3.25 and 7.3 cm respectively. All the four Pseudomonas isolates PKS10, PKM11, PKJ25, PKB27 and Pmtcc (standard check) inhibited the hyphal growth of Rhizoctonia spp. by 47.67, 49.58, 62.60, 53.28 and 55.47% respectively. One among four Pseudomonas isolates, (PKJ25) P. alcaligenes was found to suppress the growth of fungal pathogen significantly in dual culture by 62.60% and was more effective than other isolates. This study suggests that P. alcaligenes isolates might be used as potential biological control agents against plant pathogenic fungi Rhizoctonia soloni.

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Section: Resultssupporting

confidence: 75%

In vitro antagonistic activity of Pseudomonas spp. against Rhizoctonia soloni

Toppo¹,

Tiwari²

2015

Afr. J. Microbiol. Res.

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“…In other investigations, a close association was evident between phosphate-solubilizing ability and growth rate which is an indicator of active metabolism 22 , and phosphate-solubilizing activity of bacterial strains was associated with the release of organic acids and a drop in the pH of the medium 23 . Reduction in the medium pH together with an increase in halo:colony ratio and soluble phosphate indicates that the bacteria secreted organic acids to solubilize phosphate 24,25 .…”

Section: Selection Of Phosphate-solubilizing Bacteriamentioning

confidence: 61%

Identification of phosphate-solubilizing bacteria from the bamboo rhizosphere

Ruangsanka¹

2014

ScienceAsia

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Some bacteria in the root zone of plants can solubilize phosphate making it available to the plant. Bacteria associated with bamboo roots may be a good source for development of bio-fertilizer for organic crop production. Here, 24 soil samples were collected from 8 species of bamboo, and 19 isolates of bacteria with the ability to solubilize phosphate were primarily selected based on the halo-to-colony ratio in Pikovskaya's medium. Five isolates with the highest ratios were cultured in vitro in a liquid medium containing mono-calcium phosphate (Ca(H 2 PO 4 ) 2 ) and soluble phosphate was then determined. The five isolates were identified using the full 16S rDNA sequencing method. Buttiauxella izardii had the highest soluble phosphate (134 mg/l) followed by Enterobacter cancerogenus (110 mg/l), Burkholderia ubonensis (106 mg/l), and E. hormaechei (103 mg/l), whereas Burkholderia pyrrocinia was lowest (38 mg/l). The isolates were further inoculated to soil planted with baby corn (Zea mays) in a pot experiment under an open environment. The experiment was not able to confirm the ability of these bacteria isolates to promote growth of the crop. The potential use of the isolates and the methods to improve the experiments for further studies are discussed.

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“…Even though the phosphate solubilizing activity was highest at 30℃, other two strains also able to produce relatively high amount of soluble phosphorus at the 35℃ temperature (91% and 96% for PSB-1 and PSB-2 respectively when compared to the control). Generally bacteria growth was reduced at high temperature (Malboobi et al, 2009). This was more pronounced for our Burkholderia species such that phosphate solubilization dramatically decreased when incubated at 40℃.…”

Section: Assay Of Inorganic Phosphate Solubilizing Abilitiesmentioning

confidence: 99%

Evaluation of Phosphate Solubilizing Potential of Three Burkholderia Species Isolated from Green House Soils

Walpola¹,

Song²,

Keum³

et al. 2012

Korean Journal of Soil Science and Fertilizer

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Burkholderia anthina R-4183, Burkholderia diffusa R-15930 and Burkholderia stabilis LMG 14294 isolated from green house soils (Gongju-Gun area, South Korea) were characterized and their phosphate solubilizing ability was assessed. Under in vitro culture conditions, all three species were proved to be effective in solubilizing phosphates in varying degrees. Strain Burkholderia anthina exhibited the highest phosphate solubilization in NBRIP medium (665 µg ml -1 ) followed by Burkholderia diffusa (630 µg ml -1) and Burkholderia stabilis (578 µg ml -1 ). However, solubilization of FePO 4 and AlPO 4 was found to be poor in all the strains. Acidification by means of gluconic and oxalic acids accumulation in the culture medium could be the possible mechanism responsible for phosphate solubilization. Glucose at the rate of 3% was found be the best carbon source for Burkholderia anthina while other two Burkholderia species showed maximum phosphate solubilization at 2% of glucose. In the case of nitrogen sources, ammonium and nitrate were equally effective in solubilizing phosphates by Burkholderia species. Despite a slight decrease in phosphate solubilization observed at increasing temperature, all three Burkholderia species could withstand a temperature of 30-35℃, pH at the range of 7-9 and the presence of NaCl (up to 2.5%) without much compromising the phosphate solubilization. As shown with potted mung bean seedlings, all the three isolates could enhance soil fertility and plant growth indicating their great potential to be used as bio-inoculants.

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Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates

Cited by 110 publications

References 19 publications

In vitro antagonistic activity of Pseudomonas spp. against Rhizoctonia soloni

In vitro antagonistic activity of Pseudomonas spp. against Rhizoctonia soloni

Identification of phosphate-solubilizing bacteria from the bamboo rhizosphere

Evaluation of Phosphate Solubilizing Potential of Three Burkholderia Species Isolated from Green House Soils

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