Solubilization of Tricalcium Phosphate and Production of IAA by Phosphate Solubilizing Bacteria Isolated from Tea Rhizosphere Soil

Panda, Parimal; Chakraborty, Somsubhra; Ray, Deb Prasad; Mahato, Bisweswar; Pramanik, Bappa; Choudhury, Ashok

doi:10.5958/0976-4666.2015.00113.8

Cited by 5 publications

(4 citation statements)

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“…KA38 with the amount of 2.48 µg/ml. These results are in agreement with the findings of ( Panda et al., 2015 ), who reported P-solubilizing ability by bacteria in the range of 11.07 ± 0.91 - 82.77 ± 0.96 mg/l. In our study, we could also observe that most of the P-solubilizing actinobacteria belonged to the genus Streptomyces .…”

Section: Discussionsupporting

confidence: 93%

Characterization and selection of endophytic actinobacteria for growth and disease management of Tea (Camellia sinensis L.)

2022

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Endophytic microbes are vital for nutrient solubilization and uptake, growth, and survival of plants. Here, 88 endophytic actinobacteria (EnA) associated with five tea clones were isolated, assessed for their diversity, plant growth promoting (PGP), and biocontrol traits, and then used as an inoculant for PGP and disease control in host and non-host plants. Polyphasic methods, including phenotypic and genotypic characteristics led to their identification as Streptomyces, Microbacterium, Curtobacterium, Janibacter, Rhodococcus, Nocardia, Gordonia, Nocardiopsis, and Kribbella. Out of 88 isolates, 35 (39.77%) showed antagonistic activity in vitro against major fungal pathogens, viz. Fusarium oxysporum, Rhizoctonia solani, Exobasidium vexans, Poria hypobrunnea, Phellinus lamaensis, and Nigrospora sphaerica. Regarding PGP activities, the percentage of isolates that produced indole acetic acid, siderophore, and ammonia, as well as P-solubilisation and nitrogen fixation, were 67.05, 75, 80.68, 27.27, 57.95, respectively. A total of 51 and 42 isolates showed chitinase and 1-aminocyclopropane-1-carboxylic acid deaminase activity, respectively. Further, two potent Streptomyces strains KA12 and MA34, selected based on the bonitur scale, were screened for biofilm formation ability and tested in vivo under nursery conditions. Confocal laser scanning microscopy and the crystal violet staining technique revealed that these Streptomyces strains can form biofilms, indicating the potential for plant colonization. In the nursery experiment, they significantly enhanced the shoot and root biomass, shoot and root length, and leaf number in host tea plants. Additionally, treatment of tomato seeds by KA12 suppressed the growth of fungal pathogen Fusarium oxysporum, increased seed germination, and improved root architecture, demonstrating its ability to be used as a seed biopriming agent. Our results confirm the potential of tea endophytic actinobacterial strains with multifarious beneficial traits to enhance plant growth and suppress fungal pathogens, which may be used as bioinoculant for sustainable agriculture.

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Section: Discussionsupporting

confidence: 93%

Characterization and selection of endophytic actinobacteria for growth and disease management of Tea (Camellia sinensis L.)

2022

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“…In this process, negatively charged organic acids are thought to chelate Ca 2+ counter ions of phosphate [ 43 , 47 ]. A similar process was reported in other fungi such Penicillium aurantiogriseum [ 50 ] and Penicillium radicum [ 41 , 47 , 49 , 51 ].…”

Section: Discussionsupporting

confidence: 78%

“…In RP the pH of the medium of all strains, except that of BYC, was between 7 and 8, suggesting that the solubilization process does not involve the excretion of organic acids but rather that of siderophores [ 41 , 49 ]. The very efficient solubilization process of BYC might involve both the acidification of the growth medium and the excretion of siderophores.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Phosphate Solubilizing Streptomyces sp. Endemic from Sugar Beet Fields of the Beni-Mellal Region in Morocco

et al. 2021

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In the course of our research, aimed at improving sugar beets phosphorus nutrition, we isolated and characterized Streptomyces sp. strains, endemic from sugar beet fields of the Beni-Mellal region, which are able to use natural rock phosphate (RP) and tricalcium phosphate (TCP) as sole phosphate sources. Ten Streptomyces sp. isolates yielded a comparable biomass in the presence of these two insoluble phosphate sources, indicating that they were able to extract similar amount of phosphorus (P) from the latter for their own growth. Interestingly, five strains released soluble P in large excess from TCP in their culture broth whereas only two strains, BP, related to Streptomyces bellus and BYC, related to Streptomyces enissocaesilis, released a higher or similar amount of soluble P from RP than from TCP, respectively. This indicated that the rate of P released from these insoluble phosphate sources exceeded its consumption rate for bacterial growth and that most strains solubilized TCP more efficiently than RP. Preliminary results suggested that the solubilization process of BYC, the most efficient RP and TCP solubilizing strain, involves both acidification of the medium and excretion of siderophores. Actinomycete strains possessing such interesting RP solubilizing abilities may constitute a novel kind of fertilizers beneficial for plant nutrition and more environmentally friendly than chemical fertilizers in current use.

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“…In this process negatively charged organic acids are thought to chelate Ca 2+ counter ions of phosphate [43,47]. A similar process was reported in other fungi such Penicillium aurantiogriseum [51] and Penicillium radicum [41,47,49,50].…”

Section: Discussionsupporting

confidence: 58%

Isolation and Characterization of Phosphate Solubilizing Streptomyces Endemic From Sugar Beet Fields Of the Beni-Mellal Region In Morocco

Aallam¹,

Dhiba²,

Lemriss³

et al. 2021

Preprint

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In this research, we isolated and characterized Streptomyces strains, endemic from sugar beet fields of the Beni-Mellal region, able to use natural rock phosphate (RP) and tricalcium phosphate (TCP), as sole phosphate source. Ten Streptomyces isolates yielded a comparable biomass in the presence of these two insoluble phosphate sources, indicating that they were able to extract similar amount of phosphorus (P) from the latter for their own growth. Interestingly, five strains released soluble P in large excess from TCP in their culture broth whereas only two strains, BP, related to Streptomyces bellus and BYC, related to Streptomyces enissocaesilis, released a higher or similar amount of soluble P from RP than from TCP, respectively. This indicated that the rate of P released from these insoluble phosphate sources exceeded its consumption rate for bacterial growth and that most strains solubilized TCP more efficiently than RP. Preliminary results suggested that the solubilization process of BYC, the most efficient RP and TCP solubilizing strain, involves both acidification of the medium and excretion of siderophores. Actinomycete strains possessing such interesting RP solubilizing abilities may constitute a novel kind of intrans beneficial for plant nutrition and more environmentally friendly than chemical fertilizers in current use.

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Solubilization of Tricalcium Phosphate and Production of IAA by Phosphate Solubilizing Bacteria Isolated from Tea Rhizosphere Soil

Cited by 5 publications

References 0 publications

Characterization and selection of endophytic actinobacteria for growth and disease management of Tea (Camellia sinensis L.)

Characterization and selection of endophytic actinobacteria for growth and disease management of Tea (Camellia sinensis L.)

Isolation and Characterization of Phosphate Solubilizing Streptomyces sp. Endemic from Sugar Beet Fields of the Beni-Mellal Region in Morocco

Isolation and Characterization of Phosphate Solubilizing Streptomyces Endemic From Sugar Beet Fields Of the Beni-Mellal Region In Morocco

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