Solubilization of zinc phosphate by a strain of Pseudomonas fluorescens isolated from a forest soil

Simine, C. D. Di; Sayer, Jacqueline A.; Gadd, Geoffrey Michael

doi:10.1007/s003740050467

Cited by 176 publications

(94 citation statements)

References 18 publications

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“…Dissolution of the ore and other material may be due to production of organic acids, like gluconic acids (especially 2 keto gluconic acids). The zinc phosphate solubilization by Pseudomonas fluorescens was investigated by Simine et al (11). They found that gluconic acid and 2 keto…”

Section: Resultsmentioning

confidence: 99%

Assessing in vitro solubilization potential of different zinc solubilizing bacterial (zsb) isolates

Saravanan

Subramoniam

Raj

2004

Braz. J. Microbiol.

191

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Zinc solubilizing ability of Bacillus sp. and Pseudomonas sp. was assessed using zinc oxide, zinc sulphide (sphalerite) and zinc carbonate in both plate and broth assays. ZSB-O-1 (Bacillus sp.) showed highest dissolution in the zinc sulphide (Sphalerite ore), with 2.80 cm of dissolution zone and 14.50 cm 2 of area in the plate assay and 13.60 mg kg -1 of zinc in the broth assay on the 15 th day after inoculation. The ZSB-S-2 (Pseudomonas sp.) showed more solubilizing ability in the zinc oxide, with 3.30 cm clearing zone and 20.43 cm 2 area in the plate assay and 16.40 mg kg -1 of zinc in the broth assay over the same inoculation period. The isolate ZSB-S-4 (Pseudomonas sp.) has highest solubilizing potential in zinc carbonate with 6.20 cm of dissolution zone and 13.40 cm 2 area in the plate assay and 13.40 mg kg -1 of zinc in the broth assay. Thus, the solubilization potential varies among different cultures. The solubilization might be due to production of acids by the culture, since the pH of the culture broth has been shifted form 7.0-7.3 to 4.8-6.5 after 15 days of inoculation. The zinc tolerance limit for two cultures (ZSB-O-1 and ZSB-S-2) was studied and determined to be upto 100 mg kg -1 of zinc in the in vitro broth assay.

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Section: Resultsmentioning

confidence: 99%

Assessing in vitro solubilization potential of different zinc solubilizing bacterial (zsb) isolates

Saravanan

Subramoniam

Raj

2004

Braz. J. Microbiol.

191

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“…Nevertheless, only a small number of species that presently belong to the genus Pseudomonas sensu stricto are known to be phosphatesolubilizers and they all belong to rRNA group I (Palleroni et al, 1973;Palleroni, 1992). Pseudomonas putida (Viveganandan & Jauhri, 2000;Kumar & Singh, 2001;Manna et al, 2001; Villegas & Fortin, 2002), Pseudomonas aeruginosa (Musarrat et al, 2000), Pseudomonas corrugata (Pandey & Palni, 1998), Pseudomonas stutzeri (Vázquez et al, 2000) and Pseudomonas fluorescens (Di Simine et al, 1998;Deubel et al, 2000) are the most studied species. Nevertheless, many rhizospheric, phosphate-solubilizing bacterial species remain unknown and more studies are needed to reveal the high biodiversity of these bacteria.…”

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confidence: 99%

Pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro

Peix

Rivas

Mateos

et al. 2003

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A bacterial strain (designated IH5 T ), isolated from rhizospheric soil of grasses growing spontaneously in Spanish soil, actively solubilized phosphates in vitro when bicalcium phosphate was used as a phosphorus source. This strain was Gram-negative, strictly aerobic, rod-shaped and motile. The strain produced catalase, but not oxidase. Cellulose, casein, starch, gelatin, aesculin and urea were not hydrolysed. Growth was observed with many carbohydrates as the carbon source. The main non-polar fatty acids detected were hexadecenoic acid (C 16 : 1 ), hexadecanoic acid (C 16 : 0 ) and octadecenoic acid (C 18 : 1 ). The hydroxy fatty acids detected were 3-hydroxydecanoic acid (C 10 : 0 3-OH), 3-hydroxydodecanoic acid (C 12 : 0 3-OH) and 2-hydroxydodecanoic acid (C 12 : 0 2-OH). Phylogenetic analysis of 16S rRNA indicated that this bacterium belongs to the genus Pseudomonas in the c-subclass of the Proteobacteria and that the closest related species is Pseudomonas graminis. The DNA G+C content was 61 mol%. DNA-DNA hybridization showed 23 % relatedness between strain IH5 T and P. graminis DSM 11363 T . Therefore, strain IH5 T belongs to a novel species from the genus Pseudomonas, for which the name Pseudomonas rhizosphaerae sp. nov. is proposed (type strain, IH5 T =LMG 21640 T =CECT 5726 T ).Phosphorus is an essential plant nutrient that is added to soil as soluble inorganic phosphates, a large proportion of which becomes insoluble and therefore unavailable to plants (Singh & Kapoor, 1994). Many species of bacteria are able to solubilize phosphates in vitro and most of them live in the plant rhizosphere. At present, bacilli, rhizobia and pseudomonads are the most studied phosphate-solubilizers (Rodríguez & Fraga, 1999). Nevertheless, only a small number of species that presently belong to the genus Pseudomonas sensu stricto are known to be phosphatesolubilizers and they all belong to rRNA group I (Palleroni et al., 1973;Palleroni, 1992). Pseudomonas putida (Viveganandan & Jauhri, 2000;Kumar & Singh, 2001;Manna et al., 2001; Villegas & Fortin, 2002), Pseudomonas aeruginosa (Musarrat et al., 2000), Pseudomonas corrugata (Pandey & Palni, 1998), Pseudomonas stutzeri (Vázquez et al., 2000) and Pseudomonas fluorescens (Di Simine et al., 1998;Deubel et al., 2000) are the most studied species. Nevertheless, many rhizospheric, phosphate-solubilizing bacterial species remain unknown and more studies are needed to reveal the high biodiversity of these bacteria. Although the study of rhizospheric bacteria is difficult, due to the high number of bacteria present in soil, characterization and identification of these bacteria are necessary for wide ecological studies of the plant rhizosphere.During a wide study of phosphate-solubilizing, rhizospheric bacteria in soils from northern Spain, we isolated a strain that produced a yellow pigment in media that contained glucose as the carbon source and formed a transparent 'halo' around its colonies in media that contained insoluble bicalcium phosphate as the phosphorus source.A sample...

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“…The maximum solubilization efficiency was observed with ZnSF-4 having 315 % whereas minimum solubilization efficiency was found in ZnSF-3 (10 %). So far, only bacterial species belong with species of Bacillus spp and Pseudomonas spp were reported to be zinc solubilizer as they form a clear halo zone (Simine et al, 1998 andSaravanan et al, 2003) (Table 3).…”

Section: Zinc Solubilizing Capacitymentioning

confidence: 99%

Biofilm Formation of Zinc Solubilizing, Potassium Releasing Bacteria on the Surface of Fungi

Triveni¹,

Jhansi²

2017

Int.J.Curr.Microbiol.App.Sci

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Solubilization of zinc phosphate by a strain of Pseudomonas fluorescens isolated from a forest soil

Cited by 176 publications

References 18 publications

Assessing in vitro solubilization potential of different zinc solubilizing bacterial (zsb) isolates

Assessing in vitro solubilization potential of different zinc solubilizing bacterial (zsb) isolates

Pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro

Biofilm Formation of Zinc Solubilizing, Potassium Releasing Bacteria on the Surface of Fungi

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