Soluble and cell surface ICAM-3 in blood and cerebrospinal fluid of patients with multiple sclerosis: influence of methylprednisolone treatment and relevance as markers for disease activity

Abstract

The increase of ICAM-3 levels might indicate the anti-inflammatory effect of the MP treatment. It could be interesting to search for similar effects investigating the new immune modulatoring therapy forms of MS.

Interferon-� 1b leads to a short-term increase of soluble but long-term stabilisation of cell surface bound adhesion molecules in multiple sclerosis

“…In an earlier study we showed an inverse correlation of expression levels of cell surface bound AMs on peripheral blood MNC with both total number and cumulative area of all brain MRI lesions as well as with the disease duration [20]. Furthermore, we found a decrease of cICAM-1 and cICAM-3 expression levels on T cells and monocytes in CSF and blood in MS patients suffering a relapse [22,23]. In a short-term longitudinal study we also detected a further decrease of cICAM-1 expression on peripheral blood T cells in patients who suffered two relapses within the three months of observation time [23].…”

“…We also observed an inverse correlation between cICAM-1 on peripheral MNC and parameters for disease severity in brain MRI of MS patients [19]. Moreover, we found changes in the expression of cell surface bound as well as soluble ICAM-1 and ICAM-3 that might reflect the anti-inflammatory effect of high-dose methylprednisolone treatment in MS [22,24].…”

“…Our data showed that cell surface bound ICAM-1 and ICAM-3 (cICAM-1 and cICAM-3) in CSF and, to a lesser extent, in blood might be markers for clinically defined disease activity in MS [22,23]. In these studies we showed that the cell surface bound forms of AM in peripheral blood and CSF are more distinct markers for disease activity than the soluble forms.…”

“…Quantitative expression levels of cell surface bound ICAM-1 and ICAM-3 (cICAM-1 and cICAM-3) on peripheral blood MNC were measured by two colour flow cytometry (FACScan, BD Biosciences, Franklin Lakes, NJ, USA) as described previously [22,23]. MNC from peripheral blood were separated by Ficoll-Hypaque gradient and then resuspended in FACS-buffer (PBS, 2.5 % Calf serum and 0.1 % sodiumazide).…”

Soluble and Cell Surface Bound Adhesion Molecules in Blood and Cerebrospinal Fluid as Diagnostic Markers and Parameters for Disease Activity, Disease Severity and Treatment Efficacy in Patients with Multiple Sclerosis. Lösliche und Zelloberflächen-gebundene Adhäsionsmoleküle in Blut und Liquor cerebrospinalis als diagnostische Marker und Parameter für Aktivität und Schwere der Erkrankung sowie Behandlungeffektivität bei Patienten mit Multipler Sklerose

“…Against this background, we investigated whether cell surface bound AM could fulfill the criteria of objective parameters for disease activity, severity, and diagnosis in MS. Therefore, we determined the expression of cICAM-1 and cICAM-3 on MNC subsets and the percentage of CD45RA+ ICAM-3+ lymphocytes in CSF and blood of different groups of MS patients and controls in several studies [34][35][36].…”