Soluble CD44 Is a Potential Marker for the Early Detection of Head and Neck Cancer

Franzmann, Elizabeth; Reategui, Erika P.; Pedroso, Felipe E.; Pernas, Francisco G.; Karakullukcu, Bariş; Carraway, Kermit L.; Hamilton, Kara; Singal, Rakesh; Goodwin, W. Jarrard

doi:10.1158/1055-9965.epi-06-0011

Cited by 128 publications

(135 citation statements)

References 46 publications

(64 reference statements)

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“…HNSCC patients had biopsy-proven squamous cell carcinoma of the oral cavity (OC), oropharynx (OP), larynx (L), or hypopharynx (H). Controls included 39 consecutively enrolled individuals (16 from a prior study) [16] with a history of tobacco and/or alcohol use who were treated for benign diseases of the UADT. Cases included 40 consecutively enrolled, newly diagnosed HNSCC patients.…”

Section: Methodsmentioning

confidence: 99%

“…Oral rinses were collected using previously published procedures [16]. Levels of solCD44 (normal and variant isoforms) were measured using a sandwich ELISA assay (Bender MedSystems), with previously published modifications [16].…”

Section: Methodsmentioning

confidence: 99%

“…Levels of solCD44 (normal and variant isoforms) were measured using a sandwich ELISA assay (Bender MedSystems), with previously published modifications [16]. The HA test uses a competitive binding principle where the HA coated on a microtiter plate competes with HA present in a subjects'saliva (10).…”

Section: Methodsmentioning

confidence: 99%

“…The HA test uses a competitive binding principle where the HA coated on a microtiter plate competes with HA present in a subjects'saliva (10). We modified the test to correct for matrix effects as previously described for the solCD44 assay [16]. An ELISA kit was used for IL-8 (R&D Systems, Inc.) according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

“…An ELISA kit was used for IL-8 (R&D Systems, Inc.) according to the manufacturer's protocol. We performed the protein assay (Bio-Rad Laboratories) according to the manufacturer's protocol using saliva samples prepared as previously published [16]. Each sample was tested in triplicate, concentrations were averaged and the absorbances read in a microplate reader (Bio-Rad Laboratories).…”

Section: Methodsmentioning

confidence: 99%

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Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection

Pereira

Adebisi

Perez

et al. 2012

CBM

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Abstract.Background: Head and neck squamous cell carcinoma (HNSCC) is a debilitating and deadly disease largely due to late stage diagnosis. Prior work indicates that soluble CD44 (solCD44) and total protein may be useful diagnostic markers for HNSCC. In this study we combine the markers solCD44, IL-8, HA, and total protein with demographic and risk factor data to derive a multivariate logistic model that improves HNSCC detection as compared to our previous data using biomarkers alone. Methods: We performed the solCD44, IL-8, HA, and total protein assays on oral rinses from 40 HNSCC patients and 39 controls using ELISA assays. Controls had benign diseases of the upper aerodigestive tract and a history of tobacco or alcohol use. All subjects completed a questionnaire including demographic and risk factor data. Results: Depending on cancer subsite, differences between cases and controls were found for all markers. A multivariate logistic model including solCD44, total protein and variables related to smoking, oral health and education offered a significant improvement over the univariate models with an AUC of 0.853. Sensitivity ranged from 75-82.5% and specificity from 69.2-82.1% depending on predictive probability cut points. Conclusion: A multivariate model, including simple and inexpensive molecular tests in combination with risk factors, results in a promising tool for distinguishing HNSCC patients from controls. Impact: In this case-control study, the resulting observations led to an unprecedented multivariate model that distinguished HNSCC cases from controls with better accuracy than the current gold standard which includes oral examination followed by tissue biopsy. Since the components are simple, noninvasive, and inexpensive to obtain, this model combining biomarkers, risk factor and demographic data serves as a promising prototype for future cancer detection tests.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection

Pereira

Adebisi

Perez

et al. 2012

CBM

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Receptor‐Targeted Drug Delivery and the (Many) Problems We Know of: The Case of CD44 and Hyaluronic Acid

rosa

Tirelli

2018

Adv. Biosys.

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CD44 is best known for being the most common receptor of hyaluronic acid (HA). It is also a heterogeneous molecule: while its standard isoform (CD44s) is ubiquitously expressed, there are also a range of variants (CD44v), and both CD44s and CD44v undergo a variety of post‐translational modifications. The signaling roles and frequent overexpression of both CD44s and CD44v in cancer (e.g., in cancer initiating cells/cancer stem cells) has raised interest in them for diagnostic applications, but also—and most importantly for this review—as possible molecular targets in tumor therapy, with their endocytic character being a clear advantage for the intracellular release of payloads. In this area, the most popular approach employs HA‐based carriers. However, their rational design and therefore the success of HA‐based therapies are hampered by the rather limited understanding of not only the identity, but also the dynamic properties of CD44. In this review, the reader is exposed to the full breadth of the challenges that HA carriers currently face, which start at the CD44 post‐transcriptional and post‐translational heterogeneity, and also include the understanding of receptor clustering phenomena (influencing also HA avidity), as well as the evaluation of off‐target effects.

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Methylation of HPV16 and EPB41L3 in oral gargles and the detection of early and late oropharyngeal cancer

et al. 2022

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Soluble CD44 Is a Potential Marker for the Early Detection of Head and Neck Cancer

Cited by 128 publications

References 46 publications

Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection

Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection

Receptor‐Targeted Drug Delivery and the (Many) Problems We Know of: The Case of CD44 and Hyaluronic Acid

Methylation of HPV16 and EPB41L3 in oral gargles and the detection of early and late oropharyngeal cancer

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