Soluble Class I Antigen Secretion by Peripheral Blood Lymphocytes in Multiple Sclerosis

Alvarez‐Cermeño, J. C.; Brieva, José A.; Villar, Luisa María; Roldán, Eugenia Quirós; Bootello, A; González-Porqué, Pedro

doi:10.1159/000116942

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…The remarkable decrease found in serum sHLA-I titers during the phases of clinical exacerbations is of great relevance. These results, only partially in agreement with the reduced secretion of sHLA-I previously demonstrated in peripheral blood lymphocytes of MS patients regardless of disease activity (Alvarez-Cermen ˜o et al, 1993), could represent an excessive intrathecal consumption of sHLA-I antigens in patients with clinically active MS.…”

Section: Discussionsupporting

confidence: 86%

Clinical and MRI disease activity in multiple sclerosis are associated with reciprocal fluctuations in serum and cerebrospinal fluid levels of soluble HLA class I molecules

Fainardi

Granieri

Tola

et al. 2002

Journal of Neuroimmunology

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Section: Discussionsupporting

confidence: 86%

Clinical and MRI disease activity in multiple sclerosis are associated with reciprocal fluctuations in serum and cerebrospinal fluid levels of soluble HLA class I molecules

Fainardi

Granieri

Tola

et al. 2002

Journal of Neuroimmunology

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Soluble HLA class I and class II antigens in patients with multiple sclerosis

et al. 1998

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Soluble HLA class I (sHLA‐I) and soluble HLA class II (sHLA‐II) antigen levels during different stages of disease were investigated in paired serum and cerebrospinal fluid (CSF) samples from 37 patients with multiple sclerosis (MS) using ELISA and Western blot analysis. Soluble HLA‐II antigens in the serum of untreated patients with the relapsing‐remitting type of MS (RRMS) were found to be significantly elevated in acute relapse as compared to values obtained from patients under steroid treatment, in remission or healthy controls. No significant differences in circulating sHLA‐I levels could be detected. In contrast, a trend towards increased intrathecal production of sHLA‐I molecules in the CSF was observed in untreated RRMS patients in acute relapse, whereas the levels of soluble HLA‐II antigens in the CSF were below the detection limit of the ELISA method. Our observations underline the presence of systemic immune activation in MS patients, as reflected in elevated serum sHLA‐II antigen levels, and reveal a dichotomy between sHLA class I and II antigen production in the peripheral blood versus CSF in acute MS. Serial measurements of sHLA‐II antigen levels might represent a non‐invasive method to assess disease activity in MS patients.

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Influence of Ion Channel Modulation on in Vitro Interferon-γ Induced MHC Class I and II Expression on Macrophages

Zhu

Mix

Olsson

et al. 1995

Immunopharmacology and Immunotoxicology

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The in vitro effect of K+ channel blockers quinidine and verapamil, anion channel blocker SITS and K+ channel openers diazoxide, pinacidil, and BRL 38227 on interferon-gamma (IFN-gamma) induced MHC class I and II expression of Lewis rat peritoneal macrophages was investigated by cell ELISA assay. MHC class I expression was significantly enhanced by diazoxide at concentrations of 10(-5)M to 10(-6)M and by pinacidil and BRL 38227 at the concentration of 10(-6)M. MHC class II expression was enhanced by pinacidil and BRL 38227 at concentrations of 10(-5)M to 10(-6)M. The enhancing effect of pinacidil could be blocked by inhibitors of the protein kinases PKA and PKC suggesting that activation of both is required for optimum induction of MHC molecule expression. K+ and anion channel blockers were less active in modulation of MHC molecule expression. Verapamil had no influence, quinidine suppressed MHC class I expression at concentrations of 10(-4)M to 10(-5)M, and SITS suppressed MHC class I expression at the concentration of 10(-3)M. Since MHC class II expression is essential for efficient antigen presentation to T helper cells and MHC class I expression is required for target cell lysis by cytotoxic T cells, ion channel modulating drugs may be potential candidates for immunopharmacological intervention in inflammatory diseases.

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Soluble Class I Antigen Secretion by Peripheral Blood Lymphocytes in Multiple Sclerosis

Cited by 3 publications

References 13 publications

Clinical and MRI disease activity in multiple sclerosis are associated with reciprocal fluctuations in serum and cerebrospinal fluid levels of soluble HLA class I molecules

Clinical and MRI disease activity in multiple sclerosis are associated with reciprocal fluctuations in serum and cerebrospinal fluid levels of soluble HLA class I molecules

Soluble HLA class I and class II antigens in patients with multiple sclerosis

Influence of Ion Channel Modulation on in Vitro Interferon-γ Induced MHC Class I and II Expression on Macrophages

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