Soluble egg antigen of Schistosoma Haematobiuminduces HCV replication in PBMC from patients with chronic HCV infection

Awady, Mostafa K El; Youssef, Samar Samir; Omran, Moataza H; Tabll, Ashraf A.; Garf, Wael T El; Salem, Ahmed M.

doi:10.1186/1471-2334-6-91

Cited by 23 publications

(21 citation statements)

References 33 publications

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“…2A, C, and E). These dose response data are comparable to those reported for studies of SEA stimulation of professional immune cells (28)(29)(30)(31) and informed our selection of an SEA dose of 25 g/ml for 24 h.…”

Section: Resultssupporting

confidence: 73%

Schistosome Egg Antigens Elicit a Proinflammatory Response by Trophoblast Cells of the Human Placenta

et al. 2013

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f Schistosomiasis affects nearly 40 million women of reproductive age. Many of these women are infected while pregnant and lactating. Several studies have demonstrated transplacental trafficking of schistosome antigens; however, little is known regarding how these antigens affect the developing fetus and placenta. To evaluate the impact of schistosomiasis on trophoblasts of the human placenta, we isolated primary trophoblast cells from healthy placentas delivered at term. These trophoblasts were placed in culture and treated with Schistosoma japonicum soluble egg antigens (SEA) or plasma from S. japonicum-infected pregnant women. Outcomes measured included cytokine production and activation of signal transduction pathways. Treatment of primary human trophoblast cells with SEA resulted in upregulation of the proinflammatory cytokines interleukin 6 (IL-6) and IL-8 and the chemokine macrophage inflammatory protein 1␣ (MIP-1␣). Cytokine production in response to SEA was dose dependent and reminiscent of production in response to other proinflammatory stimuli, such as Toll-like receptor 2 (TLR2) and TLR4 agonists. In addition, the signaling pathways extracellular signal-regulated kinase 1/2 (ERK1/2), Jun N-terminal protein kinase (JNK), p38, and NF-B were all activated by SEA in primary trophoblasts. These effects appeared to be mediated through both carbohydrate and protein epitopes of SEA. Finally, primary trophoblasts cocultured with plasma from S. japonicum-infected pregnant women produced increased levels of IL-8 compared to trophoblasts cocultured with plasma from uninfected pregnant women. We report here a direct impact of SEA on primary human trophoblast cells, which are critical for many aspects of a healthy pregnancy. Our data indicate that schistosome antigens can activate proinflammatory responses in trophoblasts, which might compromise maternal-fetal health in pregnancies complicated by schistosomiasis.

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Section: Resultssupporting

confidence: 73%

Schistosome Egg Antigens Elicit a Proinflammatory Response by Trophoblast Cells of the Human Placenta

et al. 2013

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“…This result reflects the diagnostic value of such antibodies to detect active viral infection and agrees with previous reports on susceptibility of cells from lymphatic origin to HCV infection and their support of virus replication [22][23][39][40][41][42][43].…”

Section: Discussionsupporting

confidence: 81%

“…Due to restricted availability of primary hepatocytes, the immortalized human hepatoma cell line HepG2 was later successfully used to host HCV replication in vitro [19][20][21]. In addition, several reports demonstrated replication and assembly of HCV-genotype 4 in peripheral blood mononuclear cells (PBMCs) from Egyptian infected patients [22][23]. Herein we confirmed successful HCV propagation in both human hepatoblastoma and blood cells.…”

Section: Introductionsupporting

confidence: 65%

Schistosoma mansoni soluble egg antigens enhance HCV replication in mammalian cells

Bahgat¹,

El‐Far

Mesalam³

et al. 2010

J Infect Dev Ctries

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Background: This work demonstrates successful propagation of HCV in HepG2 and human blood cells as well as viral shedding into their culture media. The influence of Schistosoma mansoni crude soluble egg antigens (SEA) on the rate of viral propagation in both mammalian cells was also monitored. Methodology: HepG2 cells were inoculated with HCV viremic human sera and some wells were exposed to HCV infection in presence of SEA. Cells were harvested for RT-PCR and Western blotting analysis. HepG2 media was collected for HCV ELISA. Blood samples from HCV-infected humans were cultured in the presence and absence of SEA. Media were collected at different time points post culturing and subjected to HCV ELISA. Results: The ELISA concentration of HCV antigens were generally higher in media of infected HepG2 cells compared to media of control cells at all time intervals post infection. Western blots showed reactivity to immunogenic peptides of different molecular weights in lysate of infected HepG2 cells that were not evidenced in uninfected cells. In presence of SEA, RT-PCR results revealed earlier detection of viral RNA in infected HepG2 cells compared to in absence of such bilharzial antigen. Also, ELISA results revealed higher levels of detected HCV antigens in media of both infected HepG2 and blood cells cocultured with S. mansoni SEA compared to that of cultured infected cells in absence of the parasite antigens. Conclusion: HepG2 cells as well as whole blood cultures maintain HCV replication. Furthermore, SEA has the potential to enhance HCV propagation.

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“…Methods used for these assays were: (a) nested RT-PCR [10] including amplification of the highly conserved 5'-UTR sequences with nested primers in two rounds of amplification, including RT-PCR beads (Amersham Biosciences, Pittsburg, USA) in the first round, 0.2 mmol/L from each dNTP, two units of Taq DNA polymerase in the second round; (b) quantitative test of HCV RNA using C Amplicore, HCV monitor test Roche with broad dynamic range of 615 to 7,700,000 IU/mL (Amplicore, Reading, UK); and (c) HCV genotyping [11] including nested PCR amplification of HCV core gene using genotype specific primers in two rounds of amplification with 0.2 mmol/L from each dNTP and two units of Taq DNA polymerase.…”

Section: Hcv Rna Testsmentioning

confidence: 99%

Relation of interleukin-1β gene to treatment response in chronic patients infected with HCV genotype 4

Omran

Ibrahim

Youssef

et al. 2013

J Infect Dev Ctries

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Introduction: Hepatitis C virus (HCV) infection results in chronic hepatitis in more than 70% of infected patients, while 20-30% of patients recover spontaneously. This strengthens the role of the host genetic factors in either spontaneous or drug-induced viral clearance. The aim of this study was to investigate the relationship between interleukin-1β +3953 gene polymorphism and the response to interferon therapy in chronic HCV patients infected with genotype 4. Methodology: The interleukin-1β (+3953 C/T) (rs1143634) gene was amplified in 115 chronic HCV patients. Interleukin-1β single nucleotide polymorphism (SNP) plus several clinical and pathological factors were statistically analyzed in correlation with response to therapy. Results: Genotypes C/T and T/T had a significant association with non-response to treatment compared to genotype C/C, which had a strong association with response to treatment (95% confidence; 6.4884-48.5818, p = 0.0001). Furthermore, analysis of allele frequency in this cohort revealed that the T allele is associated with non-response, higher fibrosis, and higher hepatic activity, while the C allele had a significant association with sustained virologic response lower fibrosis, and lower hepatic activity (p value = 0.0001). Conclusion: This is the first study to examine the correlation between interleukin-1β (+3953 C/T) (rs1143634) gene polymorphism and the response of interferon therapy in genotype 4 HCV-infected patients. The results encourage further assessment of this SNP as a marker to predict response to therapy and disease progression, which can have major implications in saving money, time, and in avoiding unnecessary adverse effects.

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Soluble egg antigen of Schistosoma Haematobiuminduces HCV replication in PBMC from patients with chronic HCV infection

Abstract: Background: This study was conducted to examine, in vitro , the effect of soluble egg antigen (SEA) of S. haematobium on intracellular HCV RNA load in peripheral mononuclear cells (PBMC) as well as on cell proliferation in patients with chronic HCV infection.

Cited by 23 publications

References 33 publications

Schistosome Egg Antigens Elicit a Proinflammatory Response by Trophoblast Cells of the Human Placenta

Schistosome Egg Antigens Elicit a Proinflammatory Response by Trophoblast Cells of the Human Placenta

Schistosoma mansoni soluble egg antigens enhance HCV replication in mammalian cells

Relation of interleukin-1β gene to treatment response in chronic patients infected with HCV genotype 4

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