Soluble L-selectin levels during controlled ovarian hyperstimulation

Abstract

We sought to determine whether neutrophil activation, as reflected by soluble L-selectin levels, plays a role in controlled ovarian hyperstimulation (COH) and the possible correlation between soluble L-selectin and serum sex steroid levels. The study population consisted of 14 consecutive patients undergoing our routine in vitro fertilization (IVF) long gonadotropin-releasing hormone (GnRH) analog protocol. Blood was drawn three times during the COH cycle: (1) on the day when adequate suppression was obtained (Day-S); (2) on the day of, or the day prior to, human chorionic gonadotropin (hCG) administration (Day-hCG); and (3) on the day of ovum pick-up (Day-OPU). Levels of sex steroids and plasma soluble leukocyte selectin (L-selectin) were compared among the three time points. Soluble L-selectin was measured with a commercial sandwich enzyme-linked immunosorbent assay (ELISA). The results showed significantly higher levels of soluble L-selectin on Day-OPU than on Day-S and Day-hCG, and significantly lower levels on Day-hCG than Day-S. Though no significant correlations were found between soluble L-selectin and serum estradiol or hCG levels, soluble L-selectin positively correlated with serum progesterone levels. We conclude that hCG administration leads to neutrophil activation, which correlates with the degree of luteinization. Further studies are required to elucidate the relationship between the immune system and COH. These may lead to new strategies for predicting and preventing complications of COH.

Interleukin-2 and SOCS-1 proteins involvement in the pathophysiology of severe ovarian hyperstimulation syndrome-a preliminary proof of concept

“…Since, OHSS almost always develops after hCG administration or in early pregnancy, it was suggested that the hyperstimulated human ovaries produce and secrete factor(s) that cause OHSS, probably by activating a systemic inflammatory response [ 3 , 14 ]. This notion was based on the similarity of VLS to the clinical picture of OHSS [ 3 , 15 ], the significantly higher cytokine concentration in the follicular fluid at the time of oocyte retrieval in patients in whom OHSS subsequently developed [ 4 , 14 , 16 ], and the neutrophil and endothelial activations following hCG administration [ 17 – 19 ].…”

Μελέτη των ενδοθηλιακών παραγόντων (σελεκτίνη, ρεσιστίνη, οιστραδιόλη, προγεστερόνη) και των δεικτών του οξειδωτικού stress ανάλογα με τα φαρμακευτικά πρωτόκολλα σε γυναίκες που υποβάλλονται σε εξωσωματική γονιμοποίηση

“…μελετών για τη σελεκτίνη ( Orvieto R et al, 2004, Orvieto R et al 2001, Abramov Y et al 2001, Daniel Yair et al 2001)(185),(186),(187),(188) μελετών για τους παράγοντες οξειδωτικού stress (Attaran M. et al 2000, Das S et al 2006, Jana S. et al 2010, Celik E et al 2012) (213), (214),(215),(218) Στις έρευνες σχετικά με τη ρεσιστίνη μελετήθηκαν, 21 υπογόνιμες γυναίκες οι οποίες υποβλήθηκαν σε εξωσωματική γονιμοποίηση με GnRH αγωνιστή στην έρευνα του Seow. (197) Στη μελέτη του Lu συμπεριλήφθηκαν 53 γυναίκες που ακολούθησαν το πρωτόκολλο του αγωνιστή της GnRH.…”