Soluble laticifer proteins from Calotropis procera as an effective candidates for antimicrobial therapeutics

Saher, Uzma; Omer, Muhammad Ovais; Javeed, Aqeel; Anjum, Aftab Ahmad; Rehman, Kanwal; Awan, Tanzeela

doi:10.1016/j.sjbs.2023.103659

Cited by 1 publication

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References 43 publications

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“…Bacteria were first grown in LB (Luria-Bertani) broth to an absorbance of 0.8 at 600 nm. The antibacterial behavior of the peptides was studied via the agar disc diffusion assay and broth microdilution method ( 41 , 42 ). The agar plates were made by pouring 20 mL of LB broth containing 1.5% agar into 60-mm Petri dishes.…”

Section: Methodsmentioning

confidence: 99%

Antimalarial activity of cecropin antimicrobial peptides derived from Anopheles mosquitoes

Lou,

Zhang,

et al. 2024

Antimicrob Agents Chemother

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The emergence of clinically drug-resistant malaria parasites requires the urgent development of new drugs. Mosquitoes are vectors of multiple pathogens and have developed resistance mechanisms against them, which often involve antimicrobial peptides (AMPs). An-cecB is an AMP of the malaria-transmitting mosquito genus Anopheles , and we herein report its antimalarial activity against Plasmodium falciparum 3D7, the artemisinin-resistant strain 803, and the chloroquine-resistant strain Dd2 in vitro . We also demonstrate its anti-parasite activity in vivo , using the rodent malaria parasite Plasmodium berghei (ANKA). We show that An-cecB displays potent antimalarial activity and that its mechanism of action may occur through direct killing of the parasite or through interaction with infected red blood cell membranes. Unfortunately, An-cecB was found to be cytotoxic to mammalian cells and had poor antimalarial activity in vivo . However, its truncated peptide An-cecB-1 retained most of its antimalarial activity and avoided its cytotoxicity in vitro . An-cecB-1 also showed better antimalarial activity in vivo . Mosquito-derived AMPs may provide new ideas for the development of antimalarial drugs against drug-resistant parasites, and An-cecB has potential use as a template for antimalarial peptides.

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Section: Methodsmentioning

confidence: 99%