Soluble TRAIL is present at high concentrations in seminal plasma and promotes spermatozoa survival

Zauli, Giorgio; Celeghini, Claudio; Monasta, Lorenzo; Martinelli, Monica; Luppi, Stefania; Gonelli, Arianna; Grill, Vittorio; Ricci, Giuseppe; Secchiero, Paola

doi:10.1530/rep-14-0144

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…Trail gene-deficient (Trail2/2) mice were significantly reduced by 54% compared to the wild type mice, which explains the negative effects on sperm maturation in the reproductive system [30]. A high concentration of soluble Tnfsf10 was detected in the seminal plasma, which revealed a variable expression of Trail receptors in the sperm cellular fraction among different participants [36]. Those results suggest that Tnfsf10 may play an important role in sperm maturation.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of reproductive damage in the epididymis of male Kunming mice induced by chronic infection of Toxoplasma gondii PRU strain

et al. 2019

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BackgroundSome researchers have reported that Toxoplasma gondii can cause serious reproductive impairment in male animals. Specifically, T. gondii destroy the quality of sperm in the epididymis, which affects their sexual ability. However, among such studies, none have investigated the male reproductive transcriptome. Therefore, to investigate the relationship between T. gondii and sperm maturation, we infected mice with T. gondii prugniaud (PRU) strain and performed transcriptome sequencing of the epididymis.ResultsCompared with the control group, 431 upregulated and 229 downregulated differentially expressed genes (DEGs) were found (P-value < 0.05, false discovery rate (FDR) < 0.05 and |log2 (fold change)| ≥ 1). According to results of a bioinformatics analysis, Gene Ontology (GO) function is divided into three categories: cellular component, molecular function and biological process. Upon performing GO analysis, we found that some DEGs correlated with an integral part of membrane, protein complex, cell surface, ATP binding, immune system process, signal transduction and metabolic process which are responsible for the epididymal injury. DEGs were mapped to 101 unique KEGG pathways. Pathways such as cytokine-cytokine receptor interaction, glycolysis/gluconeogenesis and apoptosis are closely related to sperm quality. Moreover, Tnfsf10 and spata18 can damage the mitochondria in sperm, which decreases sperm motility and morphology.ConclusionsWe sequenced the reproductive system of male mice chronically infected with T. gondii, which provides a new direction for research into male sterility caused by Toxoplasma infection. This work provides valuable information and a comprehensive database for future studies of the interaction between T. gondii infection and the male reproductive system.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of reproductive damage in the epididymis of male Kunming mice induced by chronic infection of Toxoplasma gondii PRU strain

et al. 2019

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“…For instance, decreased expression of Bcl2l11 and Casp9 has been associated with suppression of sperm apoptosis 50 . Furthermore, exposure to recombinant tumor necrosis factor-related apoptosis-inducing ligand (encoded by Tnfsf10 ) can modulate sperm apoptosis 51 . Our findings revealed variations in the expression levels of certain genes, including Bcl2l11 and Ppp3r1, across different age groups, suggesting that changes in the miRNA profile may contribute to the aberrant expression of these target genes.…”

Section: Discussionmentioning

confidence: 99%

Investigating the impact of paternal aging on murine sperm miRNA profiles and their potential link to autism spectrum disorder

Miyahara

Tatehana

Kikkawa

et al. 2023

Preprint

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Background: Paternal aging has been consistently linked to an increased risk of neurodevelopmental disorders, including autism spectrum disorder (ASD), in offspring. While de novo mutations arising from paternal aging have been extensively studied, recent evidence has highlighted the role of epigenetic factors. We have previously revealed age-related DNA hypomethylation in mouse sperm and alterations in histone modifications during spermatogenesis. Objectives: This study aimed to investigate age-related alterations in the microRNA (miRNA) profile of mouse sperm and analyze the target genes regulated by differentially expressed miRNAs. Materials and Methods: Microarray analyses were conducted to identify differentially expressed miRNAs in sperm samples from mice at different ages: 3 months (3M), over 12M, and beyond 20M. The expression levels of genes potentially regulated by these miRNAs were also accessed. Comprehensive bioinformatic analyses were performed to unravel the intricate relationship between differentially expressed miRNAs and their target genes. Results: We identified 26 miRNAs exhibiting differential expression between the 3M and 20M, 34 miRNAs between the 12M and 20M, and 2 miRNAs between the 3M and 12M mice. Notably, the target genes regulated by these differentially expressed miRNAs were significantly associated with apoptosis and ferroptosis pathways. Some of these target genes were relevant to the nervous system and potentially involvement in ASD. Discussion: The findings suggest that aging induces alterations in the sperm miRNA profiles . Importantly, the target genes regulated by these differentially expressed miRNAs are associated with cell death and ASD, implying a potential link between paternal aging and an increased risk of neurodevelopmental disorders. Conclusion: The observed age-related changes in sperm miRNA profiles have the potential to impact sperm quality and subsequently affect offspring development. Further validation and through investigations are necessary to gain a comprehensive understanding of the precise effects of paternal aging on subsequent generations.

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“…Although binding of TRIAL to its receptors induces an apoptotic response, other apoptosis‐independent actions have been reported. The expression of TRAIL receptors (TRAIL1, TRAIL2, TRAIL3 and TRAIL4) was studied by flow cytometry (Zauli et al., ), and the presence of the soluble anti‐inflammatory cytokine was investigated. This study provided evidence of pro‐survival and anti‐inflammatory actions for TRAIL.…”

Section: Single‐cell Analysis In Sperm Proteomics: Flow Cytometrymentioning

confidence: 99%

Flow cytometry in Spermatology: A bright future ahead

Ortega-Ferrusola

Gil

Rodriguez‐Martinez

et al. 2017

Reprod Domestic Animals

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Techniques such as mass spectrometry have led to unprecedented knowledge of the proteins that are present in the spermatozoa of humans and other mammals. However, in spite of their high-throughput and fractioning techniques, most of the techniques in use only offer average values for the entire sperm population. Yet, ejaculate is very heterogeneous, and average values may mask relevant biological information.The application of flow cytometry may overcome this disadvantage, allowing proteomic analysis at the single-cell level. Moreover, recent advances in cytometry, allowing multiple analyses within a single cell combined with powerful statistical tools, as an expanding subfield in spermatology, are described. The increased use of advanced flow cytometers in andrology laboratories will allow the rapid development of multiparametric, multicolour flow cytometry in andrology that will expand the clinical applications and research possibilities of flow cytometry-based proteomic approaches, especially in the subfields of clinical andrology and sperm biotechnology.

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Soluble TRAIL is present at high concentrations in seminal plasma and promotes spermatozoa survival

Cited by 7 publications

References 42 publications

Transcriptomic analysis of reproductive damage in the epididymis of male Kunming mice induced by chronic infection of Toxoplasma gondii PRU strain

Transcriptomic analysis of reproductive damage in the epididymis of male Kunming mice induced by chronic infection of Toxoplasma gondii PRU strain

Investigating the impact of paternal aging on murine sperm miRNA profiles and their potential link to autism spectrum disorder

Flow cytometry in Spermatology: A bright future ahead

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