Solute Transport in Cyclically Deformed Porous Tissue Scaffolds with Controlled Pore Cross-Sectional Geometries

Buijs, Jorn op den; Lu, Lichun; Jorgensen, Steven M.; Dragomir-Daescu, Dan; Yaszemski, Michael J.; Ritman, Erik L.

doi:10.1089/ten.tea.2008.0382

Cited by 7 publications

(8 citation statements)

References 60 publications

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“…This is likely due to a reduction in pore size and change in pore geometry. In a recent study, Den Buijs et al (2009) demonstrated that pore shape and size greatly influenced solute transport rate and spatial distribution in cyclically-deformed scaffolds.…”

Section: Discussionmentioning

confidence: 99%

An in vitro model for the pathological degradation of articular cartilage in osteoarthritis

Grenier

Bhargava

Torzilli

2014

Journal of Biomechanics

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The objective of this study was to develop an in vitro cartilage degradation model that emulates the damage seen in early-stage osteoarthritis. To this end, cartilage explants were collagenase-treated to induce enzymatic degradation of collagen fibers and proteoglycans at the articular surface. To assess changes in mechanical properties, intact and degraded cartilage explants were subjected to a series of confined compression creep tests. Changes in extracellular matrix structure and composition were determined using biochemical and histological approaches. Our results show that collagenase-induced degradation increased the amount of deformation experienced by the cartilage explants under compression. An increase in apparent permeability as well as a decrease in instantaneous and aggregate moduli were measured following collagenase treatment. Histological analysis of degraded explants revealed the presence of surface fibrillation, proteoglycan depletion in the superficial and intermediate zones and loss of the lamina splendens. Collagen cleavage was confirmed by the Col II–¾Cshort antibody. Degraded specimens experienced a significant decrease in proteoglycan content but maintained total collagen content. Repetitive testing of degraded samples resulted in the gradual collapse of the articular surface and the compaction of the superficial zone. Taken together, our data demonstrates that enzymatic degradation with collagenase can be used to emulate changes seen in early-stage osteoarthritis. Further, our in vitro model provides information on cartilage mechanics and insights on how matrix changes can affect cartilage’s functional properties. More importantly, our model can be applied to develop and test treatment options for tissue repair.

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Section: Discussionmentioning

confidence: 99%

An in vitro model for the pathological degradation of articular cartilage in osteoarthritis

Grenier

Bhargava

Torzilli

2014

Journal of Biomechanics

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“…On the other hand, it is necessary for cells within the scaffold to receive nutrients and discard waste material in order to maintain their viability, a property governed by interstitial fluid flow which is dependent on the scaffold permeability. Interstitial fluid flow in a scaffold is also influenced by cyclic strain [50], however, and is therefore also dependant on the elasticity of the scaffold. Clearly therefore, there are numerous interlinked mechanobiological parameters which need to be considered in the design and development of viable tissue engineered blood vessels.…”

Section: Intimal Hyperplasia In Tissue Engineered Vascular Graftsmentioning

confidence: 99%

Multiscale Modeling in Vascular Disease and Tissue Engineering

Zahedmanesh

Lally

2012

Multiscale Computer Modeling in Biomechanics and Biomedical Engineering

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The human body, and hence the vascular system, is by its very nature a dynamic multiscale hierarchial system. This multiscale nature encompasses different length scales, from molecular and cellular levels to the tissue and organ level, as well as different physical phenomena, such as mechanical, biological and chemical processes. In arteries, vascular cells alter their growth, phenotype and extracellular matrix production in response to macro mechanical changes. These cell level events can in turn accumulate and emerge at the tissue level as pathological conditions such as atherosclerosis and intimal hyperplasia. These cardiovascular diseases evolve through adaptation of cells and tissues over days to months also demonstrating the multiscale nature of vascular diseases with respect to time. The challenge in vascular multiscale modelling is to create a framework which can incorporate the key mechanical, biological and chemical characteristics of this complex system at these various space and time scales to successfully capture the long-term behaviour of the system. Such a framework can then be used to gain additional insights with regards to pathological conditions within the vascular system and to improve the design of medical devices used to treat such pathologies. In the following chapter, a review will be presented of some relevant studies reported in literature which have used multiscale modelling approaches to elucidate the growth and remodelling mechanisms underlying vascular diseases, such as atherosclerosis, in-stent restenosis and intimal hyperplasia.

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“…On the other hand, the elasticity of the construct, usually measured as compliance in the context of vascular grafts/scaffolds, plays a pivotal role in vascular scaffold remodelling as cyclic strain has a significant anti-proliferative and proapoptotic influence on VSMCs (Colombo 2009). In addition, fluid flow in a scaffold is influenced by cyclic strain (Buijs et al 2009) and is therefore also dependant on the elasticity of the scaffold when it is subject to pulsatile pressure in the arterial system. The importance of the role of compliance is further supported by the outcome of vascular graft implantations that suggest a direct relation between the compliance of vascular grafts and their long-term patency (Salacinski et al 2001).…”

Section: Introductionmentioning

confidence: 99%

A multiscale mechanobiological modelling framework using agent-based models and finite element analysis: application to vascular tissue engineering

Zahedmanesh

Lally

2011

Biomech Model Mechanobiol

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Computational models of mechanobiological systems have been widely used to provide insight into these systems and also to predict their behaviour. In this context, vascular tissue engineering benefits from further attention given the challenges involved in developing functional low calibre vascular grafts with long-term patency. In this study, a novel multiscale mechanobiological modelling framework is presented, which takes advantage of lattice-free agent-based models coupled with the finite element method to investigate the dynamics of VSMC growth in vascular tissue engineering scaffolds. The results illustrate the ability of the mechanobiological modelling approach to capture complex multiscale mechanobiological phenomena. Specifically, the framework enabled the study of the influence of scaffold compliance and loading regime in regulating the growth of VSMCs in vascular scaffolds and their role in development of intimal hyperplasia (IH). The model demonstrates that low scaffold compliance compared to host arteries leads to increased luminal ingrowth and IH development. In addition, culture of a tissue-engineered blood vessel under a pulsatile luminal pressure reduced luminal ingrowth and enhanced collagen synthesis within the scaffold compared to non-pulsatile culture. The mechanobiological framework presented provides a robust platform for testing hypotheses in vascular tissue engineering and lends itself to use as an optimisation design tool.

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Solute Transport in Cyclically Deformed Porous Tissue Scaffolds with Controlled Pore Cross-Sectional Geometries

Cited by 7 publications

References 60 publications

An in vitro model for the pathological degradation of articular cartilage in osteoarthritis

An in vitro model for the pathological degradation of articular cartilage in osteoarthritis

Multiscale Modeling in Vascular Disease and Tissue Engineering

A multiscale mechanobiological modelling framework using agent-based models and finite element analysis: application to vascular tissue engineering

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