Solute Uptake of <i>Acer pseudoplatanus</i> Cell Suspensions during Recovery from Gas Shock

Thoiron, Bernard; Thoiron, A.; Guiel, Jeanne Le; Lüttge, Ulrich; Thellier, Michel

doi:10.1111/j.1399-3054.1979.tb02632.x

Cited by 28 publications

(14 citation statements)

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“…The cell suspensions ofAcer pseudoplatanus L. were grown in the nutrient medium of Lescure (17), under the general conditions described previously (1,22). The specific problem of cell impalement in a shielded microscopic chamber has also been discussed previously (1).…”

Section: Methodsmentioning

confidence: 99%

Voltage Noise in Acer pseudoplatanus Cells

Alexandre¹,

Lassalles²,

Thellier³

1985

Plant Physiol.

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The present contribution is devoted to studying the electrical noise of Acer pt cells in culture suspensions. Sponbtneous voltage noise of the cells was recorded by means of a microelectrode inserted in the vacuole. The small signal impedance of the cell was measured so that it was possible to study the intensity spectra of the noise. We recorded intensity spectra with cells incubated in 10' molar gramicidin A. Difference spectra showed charcteristics of a channel noise. By using the calculated conductance of Amcidin A in an artificial membrane, and by simplfyig assuptions for the ionic transports through plasmalemma and tonoplast, we were able to estimate the electrochemical potential difference for K ions across the plasmalemma (3.2 1 1 millivolt).Analysis of random fluctuations ('noise' analysis) of the electrical parameters of a cell (transmembrane electric potential and current) is now widely used in animal electrophysiology, especially with nerve cells (5).Noise analysis experiments have also been performed with plant cells (1,7,16,19,24). However, the models of transmembrane ionic transports in these cells are still far from being as good as those for the nerve cells, which make interpretation of the data rather cumbersome. We usually try to correlate variations between a specific parameter of the external medium and the modification in the noise spectra. The existence of good models for ion carriers (channels) in the cells, and the knowledge of how the opening and closure ofthe channels are triggered, has been particularly helpful to detect this type of noise in the recorded spectra. Noise induced by active transport is not as easy to characterize. Recently, Ross and Dainty (personal communication) have shown evidence that a low-frequency component in the voltage noise spectra of Chara corallina could possibly be associated with the active transport of H+ occurring in these cells. In this article, we worked on Acer pseudoplatanus. The electrical and ionic parameters of this cell have already been studied in great detail (9,20,21). We recorded the electrical noise of the cell and although no reproducible structure in the noise spectra was detected in the range 0.5 to 100 Hz, a significant increase ofthe noise occurred for these frequencies. We also used gramicidin A to modify this noise spectra.Gramicidin A is known to induce channels in artificial lipid bilayers (15). At a concentration of I0-' M, gramicidin A modified the noise spectra recorded on Acer cell. By assuming that characteristics are similar for gramicidin A in the cell membrane ' Funded by grants from the Centre National de la Recherche Scientifique (UA 203 and RCP 08726). and in the artificial membrane, we were able to deduce the number of induced channels per cell. Thus, as will be shown in this article, gramicidin A, at low concentrations can be used as a probe for measuring the electrochemical potential difference ( V 2 -V,,,) across the plasmalemma, provided that one can find an ionic carrier, to be incorporated in the cell memb...

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Section: Methodsmentioning

confidence: 99%

Voltage Noise in Acer pseudoplatanus Cells

Alexandre¹,

Lassalles²,

Thellier³

1985

Plant Physiol.

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“…(13,14). Our procedure (15) involved pipetting aliquots to test tubes for imposition of heat stress.…”

Section: Introductionmentioning

confidence: 99%

“…Another inherent feature of tissue culture systems is the routine handling and manipulation involved in the transfer and treatment ofcells. Tissue handling has been shown to induce subtle but substantial metabolic changes (13,14), some ofwhich could influence heat tolerance.In this paper, we describe effects of suspension culture age and handling of the heat tolerance of pear cells. (13,14).…”

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confidence: 99%

“…Tissue handling has been shown to induce subtle but substantial metabolic changes (13,14), some ofwhich could influence heat tolerance.…”

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confidence: 99%

“…Knowledge initial study (15). The culture medium and most methods (culture maintenance, growth conditions and measurement, heat stress treatment, and viability tests) were as described previously (15 (13,14). Our procedure (15) involved pipetting aliquots to test tubes for imposition of heat stress.…”

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Heat Stress Responses in Cultured Plant Cells

Wu¹,

Wallner²,

Waddell³

1984

Plant Physiol.

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The pipetting ofpear (Pyrus communis cv Bartlett) suspension cultures was followed by a substantial but transient decrease in heat sensitivity. During a culture cycle, pear cells were most sensitive to heat at day 3, which coincided with the period of most active cell division. To minimize serious artifacts, the influence of culture handling and age on parameters such as heat sensitivity must be standardized.In the study of complex phenomena such as temperature response, the convenience, simplicity, and uniformity of cell cultures make them valuable experimental systems. Although effects ofheat stress on photosynthesis are critical to whole plants, the absence of chloroplasts in simple cell cultures may further facilitate close examination of other metabolic responses. In considering tissue cultures as model systems, it is important to note that certain aspects of cultured cell response to heat are similar to those of intact plant tissues. Heat stress disrupts polysomes in cells of intact pear fruit (10) and in cultured pear fruit cells (Romani, personal communication). In both intact organs and tissue cultures, heat shock induces acclimation (8,12) and the synthesis of heat-shock proteins (1, 7). However, it is clear that the isolation and culture of plant cells alters physiological characteristics which may influence high temperature response. For example, batch-propagated suspension cultures are characterized by stages ofrelatively high mitotic activity (5). This is relevant to the study of heat stress because certain dividing cells are especially susceptible to heat injury (9, 1 1). Therefore, it is necessary to consider the relationship of culture age to heat sensitivity. Another inherent feature of tissue culture systems is the routine handling and manipulation involved in the transfer and treatment ofcells. Tissue handling has been shown to induce subtle but substantial metabolic changes (13,14), some ofwhich could influence heat tolerance.In this paper, we describe effects of suspension culture age and handling of the heat tolerance of pear cells. (13,14). Our procedure (15) involved pipetting aliquots to test tubes for imposition of heat stress. We found that handling (transfer by pipette) influenced heat sensitivity (Fig. 1)

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Maple (Acer spp.)

Morselli

1989

Trees II

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Solute Uptake of Acer pseudoplatanus Cell Suspensions during Recovery from Gas Shock

Cited by 28 publications

References 10 publications

Voltage Noise in Acer pseudoplatanus Cells

Voltage Noise in Acer pseudoplatanus Cells

Heat Stress Responses in Cultured Plant Cells

Maple (Acer spp.)

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