The enzyme system mimicking Implication (IMPLY) and Inhibition (INHIB) Boolean logic gates has been designed. The same enzyme system was used to operate as the IMPLY or INHIB gate simply by reformulating the input signals. The optical analysis of the logic operation confirmed the output generation as expected for the studied logic gates. The conceptual approach to the IMPLY and INHIB logic gates allows their construction with many other enzymes operating in a similar way. Molecular [1-4] and biomolecular [5] computing, as a subarea of unconventional computing, [6] have attracted high attention and rapidly progressed in the last two decades. Enzyme-based logic systems, [7] together with DNA/RNA computing systems, [8-10] are the most important areas of research in the general framework of the biomolecular information processing systems. Enzymecatalyzed reactions, including sophisticated multi-step/multienzyme reaction cascades, have been used to mimic almost all known Boolean logic gates, [7,11] such as Yes (Identity), Not (Inversion), OR, NOR, XOR, NXOR, AND, NAND, INHIB, including reversible logic gates, [12,13] such as Feynman, Toffoli, Fredkin and Peres gates. While the vast majority of Boolean logic gates mimicked with enzyme systems have been already formulated, [7,11] optimized [14-17] and reported for various applications, [18-20] some unique logic operations still require additional studies. Particularly, Implication (IMPLY) logic gate (also named "material implication") has not achieved enough attention. This gate was first described more than 100 years ago and recognized as a basic logic operation. [21] For some technical reasons this gate was ignored in digital electronics for many years, but recently interest to this gate has been returned based on its straightforward realization in memristive switches. [22] The IMPLY logic gate has been realized in many chemical systems, [23-36] including biomolecular systems based on DNA molecules, [37-49] particularly operated as cell biology processes. [50] However, the IMPLY gate was rarely demonstrated with enzyme-catalyzed reactions. [51] The present study aimed at the general approach to the IMPLY logic gate. This approach allows the use of various