Solution properties of exocellular microbial polysaccharides. 3. Light scattering from gellan and from the exocellular polysaccharide of Rhizobium trifolii (strain TA-1) in the ordered state

Dentini, Mariella; Coviello, Tommasina; Burchard, Walther; Crescenzi, V.

doi:10.1021/ma00189a028

Cited by 108 publications

(74 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A further demonstration that the latter state is built up by at least two strands was given by Dentini et al using light-scattering data, showing an experimentally observed mass per unit length in 0.1M NaCl and 25°C that is double with respect to that of single chains at 50°C. 8 Furthermore, the same authors observed that the stability of gellan-ordered structure may be increased going from water to heavy water solution, confirming the important role played by water molecules as stabilizers of the ordered conformation of gellan, in agreement with the double-helix model proposed on the basis of x-ray fiber diffraction data. [2][3][4] …”

Section: Summary Of Dichroism Measurementssupporting

confidence: 64%

“…[2][3][4] In aqueous solutions at much lower gellan concentrations, a clear transition was detected by various techniques (viscosity, chiroptical spectroscopy, calorimetry, light scattering, and conductivity), with a strong dependence upon solvent, ionic strength, and polymer concentration. [5][6][7][8][9][10][11] It therefore seems interesting to investigate further the structure of gellan in solutions where only an equilibrium between single chains and double helices might be thought to exist, without the presence of the perturbing effects due to the gel formation. This study was performed by means of high field nmr of very dilute aqueous solutions of gellan as a function of temperature.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The structure of gellan in dilute aqueous solution

et al. 2000

Self Cite

View full text Add to dashboard Cite

Section: Summary Of Dichroism Measurementssupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

The structure of gellan in dilute aqueous solution

et al. 2000

Self Cite

View full text Add to dashboard Cite

“…Upon addition of mono-, di-or trivalent cations, gelation (sol-gel transition) occurs as the helices aggregate into junction zones which are linked into a three dimensional network via the coiled part of the molecule. [28][29][30][31] This gel formation differs greatly from that of alginate, where the divalent cations bind guluronic acids blocks (G-blocks) and form eggbox structures between chains. [32][33][34] The gelling behavior of the blended bioink is illustrated using the bioprinted auricular cartilage as an example ( Figure 1 ).…”

Section: Bioink Crosslinkingmentioning

confidence: 99%

Bioprinting Complex Cartilaginous Structures with Clinically Compliant Biomaterials

Kesti

Eberhardt²,

Pagliccia

et al. 2015

Adv Funct Materials

198

149

View full text Add to dashboard Cite

wileyonlinelibrary.comwith rapid production of patient-specifi c grafts, allowing precise control over internal and external architecture and customized mechanical properties. These techniques can be used for printing biological materials together with living cells, hence the term "bioprinting." 3D bioprinting offers researchers a unique way of depositing cell-laden biocompatible materials, the so-called bioinks, in high-resolution structures with a line thickness on the order of hundreds of microns. Due to the promise of such a technology, several commercial bioprinters have entered the market and bioinks are the subject of intense investigation. [1][2][3] Bioink formulation is often considered one of the most critical aspects of high-resolution cellular bioprinting.Cellular printing requires a bioink with two key properties, namely printability and cytocompatible crosslinking. The identifi cation of printable polymeric systems is mainly done through rheological evaluation of a material's shear thinning behavior and shear recovery. Shear thinning correlates directly with a bioink's ability to be extruded at low pressure (<3 bar), something which ensures high postprinting cell viability. [ 4 ] Shear recovery, on the other hand, relates to the ink's resistance to fl ow after printing, which ensures high fi delity of the printed structure. The presence of cells, however, greatly restricts the crosslinking options as physiologic temperature and pH need to be maintained and harsh chemicals avoided. Hydrogel bioinks can be crosslinked via covalent or physical interactions or a combination thereof. Ultraviolet light initiated crosslinking of (meth)acrylated polymers has been used most often in bioinks, but the presence of potentially toxic monomers and photoinitiators may complicate clinical translation. [5][6][7][8] Physically crosslinked gelation based on temperature, hydrophobic/hydrophilic or ionic interactions has been utilized for precrosslinking of several bioink materials including poly( N -isopropylacrylamide) conjugated hyaluronan (HA-pNIPAAm), [ 9 ] gelatin, [ 10,11 ] alginate, [ 12 ] and gellan. [ 13 ] Precrosslinking before printing or directly during deposition to stabilize the printed lines is generally followed by a fi nal crosslinking which further increases the mechanical properties and stabilizes the whole structure.For cartilage engineering applications, natural polymers from animal or plant sources including alginate, collagen, gelatin, Bioprinting is an emerging technology for the fabrication of patient-specifi c, anatomically complex tissues and organs. A novel bioink for printing cartilage grafts is developed based on two unmodifi ed FDA-compliant polysaccharides, gellan and alginate, combined with the clinical product BioCartilage (cartilage extracellular matrix particles). Cell-friendly physical gelation of the bioink occurs in the presence of cations, which are delivered by co-extrusion of a cation-loaded transient support polymer to stabilize overhanging structures. Rheological properties of...

show abstract

“…For hard spheres the integral equation (14) can be solved analytically. The result given by Guinier and Fournier K is not reproduced here.…”

Section: Some General Remarksmentioning

confidence: 99%

“…The result given by Guinier and Fournier K is not reproduced here. At present there exists no exact solution of eqn (14) for partially interpenetrating particles (e.g. star-branched macromolecules or microgels).…”

Section: Some General Remarksmentioning

confidence: 99%

Light Scattering Techniques

Burchard

1994

Physical Techniques for the Study of Food Biopolymers

View full text Add to dashboard Cite

Solution properties of exocellular microbial polysaccharides. 3. Light scattering from gellan and from the exocellular polysaccharide of Rhizobium trifolii (strain TA-1) in the ordered state

Cited by 108 publications

References 9 publications

The structure of gellan in dilute aqueous solution

The structure of gellan in dilute aqueous solution

Bioprinting Complex Cartilaginous Structures with Clinically Compliant Biomaterials

Light Scattering Techniques

Contact Info

Product

Resources

About