2003
DOI: 10.1074/jbc.m210191200
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Solution Structure and Function of the “Tandem Inactivation Domain” of the Neuronal A-type Potassium Channel Kv1.4

Abstract: Cumulative inactivation of voltage-gated (Kv) K؉ channels shapes the presynaptic action potential and determines timing and strength of synaptic transmission. Kv1.4 channels exhibit rapid "ball-and-chain"-type inactivation gating. Different from all other Kv␣ subunits, Kv1.4 harbors two inactivation domains at its N terminus. Here we report the solution structure and function of this "tandem inactivation domain" using NMR spectroscopy and patch clamp recordings. Inactivation domain 1 (ID1, residues 1-38) consi… Show more

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Cited by 41 publications
(43 citation statements)
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“…The initial chemical shift index-based structure (Fig. S3, Upper) comparison indicated potential differences to a structure of an elongated construct reported earlier [1KN7 (22)]. We therefore decided to obtain an independent 3D starting structure by in silico structure predictions with CS-Rosetta and CHESHIRE to be used for the docking experiments with the heme moiety.…”
Section: Resultsmentioning
confidence: 99%
“…The initial chemical shift index-based structure (Fig. S3, Upper) comparison indicated potential differences to a structure of an elongated construct reported earlier [1KN7 (22)]. We therefore decided to obtain an independent 3D starting structure by in silico structure predictions with CS-Rosetta and CHESHIRE to be used for the docking experiments with the heme moiety.…”
Section: Resultsmentioning
confidence: 99%
“…3). Among the inactivating K ϩ currents known to be expressed in DRG neurons, only Kv1 subtypes are sensitive to TEA blockade (Wissmann et al, 2003), thus the most likely target for the KW-7158 facilitatory effect is a Kv4 K ϩ channel subtype (Pongs, 1992;Tkatch et al, 2000).…”
Section: Discussionmentioning
confidence: 99%
“…2D). 5) Treatment of Rg 3 to hKv1.4D2-61 channels produced larger inhibitions of non-inactivating plateau than peak outward K ϩ currents in a concentration-dependent manner. Mutation of K531 hKv1.4D2-61 to K531Y hKv1.4D2-61 and raising extracellular [K ϩ ] o abolished Rg 3 -mediated inhibitions of non-inactivating plateau outward K ϩ currents (Figs.…”
Section: Discussionmentioning
confidence: 99%
“…2D, Con). 5,9) In the absence of Rg 3 , both hKv1.4 and hKv1.4D2-61 channel currents were elicited by voltage pulses more positive than Ϫ40 mV, and current amplitude increased linearly with further depolarization (Figs. 2E, F; * pϽ0.05 * * pϽ0.01 at ϩ60 mV, nϭ7-8).…”
Section: Rg 3 Preferentially Inhibits Non-inactivating Rather Than Pementioning
confidence: 99%
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