Anew azo ligand (E)-2-((4-fluoro phenyl)diazenyl)-4,5-bis(4-methoxy phenyl)-1H-imidazolehas been synthesized from coupling 4,5-bis(4-methoxyphenyl)imidazole with para-chloro-anline. The structures of the new ligand and their transition metal complexes ware investigated and characterized using different physicochemical studies as elemental analysis, IR spectra, 1 HNMR,, UV-Vis spectra, mass spectra, Molar conductance ,and magnetic susceptibility .The complexes were found to have the general formula [ML 2 Cl 2 ]. H 2 O where M = Co(II), Ni(II), Cu(II) ,Mn(II),and Fe(II), [MLCl 2 ].H 2 Owhere M=Zn(II),Cd(II),Hg(II), and Pd(II). The IR results demonstrate that the co-ordination sites are the azomethine nitrogen of the imidazole ring and azo nitrogen atom of the azo ligand. The electronic spectral and magnetic measurement data indicate that the complexes exhibit octahedral geometry,except the Zn(II),Cd(II),andHg(II) complexes suggest tetrahedral and Pd(II) a square planar geometry.The cytotoxicity of Pd(II) complex on human Lung Cancer (SCLC) and normal cells.