Solvent and Rotating Bed Reactor Extraction with One- and Two-Phase Solvents Applied to Bilberries (Vaccinium myrtillus) for Isolating Valuable Antioxidants

Abstract

Extraction of antioxidants from bilberries using solvent extraction and the novel rotating bed reactor (RBR) both with one-phase (ethanol and water) and two-phase (ethanol and water +salt) solvents was studied. Solids, ethanol, and temperature settings in an experimental design were monitored for 1 h. The measured responses were (1) polyphenol concentration, (2) visible-near infrared spectra, and (3) HPLC measurement. The (1) responses were used for making response surfaces in time and the spectra (2) could confirm these results. The HPLC results (3) confirmed the results of 1 and 2 but were found unsuitable for online monitoring. The RBR was better than traditional extraction and 16 min sufficed. The response surfaces showed an optimal concentration of ethanol, temperatures above 50°C gave the best results, and high loads of solid were beneficial. Two-phase extraction was less efficient. The methodology could be transferred to larger scale extraction systems to improve yield and save on reagents/energy cost.

Yabanmersi̇ni̇ (Vacci̇ni̇um Myrti̇llus L.) Meyve Özü'nün Mi̇de Kanseri̇, Ags Hücre Hatti Üzeri̇nde Anti̇oksi̇dan Akti̇vi̇tesi̇ Ve Anti̇-Kanser Etki̇leri̇

“…These polyphenols, anthocyanins (delphinidin, cyanidin, petunidin, pelargonidin, peonidin and malvidin with the sugar part consists of glucose, arabinose, and galactose) the main ones, which belong to the flavonoid group and are responsible for the pigmentation of these fruits. Phenolic acids (caffeic, p-coumaric, ferulic, chlorogenic acids), flavonols (quercetin, myricetin), and flavonol (catechin) have been determined in fruits [5][6][7]. Because of these polyphenols, the fruits have antioxidant, astringent, antibacterial, and antiseptic properties.…”