Solvent Dependence of the Fluorescence Lifetimes of Xanthene Dyes

Magde, Douglas; Rojas, Gail E.; Seybold, Paul G.

doi:10.1111/j.1751-1097.1999.tb08277.x

Cited by 479 publications

(289 citation statements)

References 57 publications

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“…32 On the other hand, the non-radiative process involving rotation of diethylamino groups is sensitive to the solvent viscosity. 32,33 Although the fluorescence dynamics of RhB depends on not only the viscosity but also other solvent properties such as polarity and hydrogen-bonding nature, the solvent viscosity can be approximately evaluated from an analysis of the fluorescence lifetime of RhB. 32,33 RhB isothiocyanate easily binds to the surface AP layer, and it can be used as a viscosity probe inside the pores.…”

Section: Introductionmentioning

confidence: 99%

“…32,33 Although the fluorescence dynamics of RhB depends on not only the viscosity but also other solvent properties such as polarity and hydrogen-bonding nature, the solvent viscosity can be approximately evaluated from an analysis of the fluorescence lifetime of RhB. 32,33 RhB isothiocyanate easily binds to the surface AP layer, and it can be used as a viscosity probe inside the pores. Accordingly, RhB was chosen to probe the microviscosity of supercooled water confined inside AP-modified silica mesopores.…”

Section: Introductionmentioning

confidence: 99%

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Microviscosity of Supercooled Water Confined within Aminopropyl-modified Mesoporous Silica as Studied by Time-resolved Fluorescence Spectroscopy

et al. 2012

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The fluorescence dynamics of rhodamine B (RhB) immobilized on the pore surface of aminopropyl (AP)-modified mesoporous silica (diameter of the silica framework, 3.1 nm) was examined at temperatures between 293 and 193 K to study the microviscosity of supercooled water confined inside the pores. The mesoporous silica specimen with a dense AP layer (2.1 molecules nm -2 ) was prepared, and RhB isothiocyanate was covalently bound to part of the surface AP groups. The fluorescence lifetime of the surface RhB increased with decreasing temperature from 293 to 223 K, indicating that freezing of the confined water did not occur in this temperature range. The microviscosity of the supercooled confined water was evaluated from an analysis of the lifetime data based on a frequency-dependent friction model.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microviscosity of Supercooled Water Confined within Aminopropyl-modified Mesoporous Silica as Studied by Time-resolved Fluorescence Spectroscopy

et al. 2012

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“…The fluorescence emission can be characterized by lifetime, wavelength, or polarization to provide structural and functional information. The fluorescence lifetime can report on the type of fluorophore, its orientation, its interaction with other molecules 1 or the properties of its environment, including the refractive index [2][3][4][5][6] and the viscosity. 7 While some molecules exhibit a viscosity dependent fluorescence lifetime due to the competition of nonradiative de-excitation pathways such as internal twisting with radiative de-excitation, 8 in general the fluorescence lifetime is independent of solvent viscosity.…”

Section: Introductionmentioning

confidence: 99%

Wide-field time-resolved fluorescence anisotropy imaging (TR-FAIM): Imaging the rotational mobility of a fluorophore

Siegel

Suhling

Lévêque‐Fort

et al. 2003

Review of Scientific Instruments

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We report a picosecond time-gated fluorescence lifetime imaging ͑FLIM͒ system extended to perform time-resolved fluorescence anisotropy imaging ͑TR-FAIM͒. Upon excitation with linearly polarized laser pulses, the parallel and perpendicular components of the fluorescence emission from a sample are imaged simultaneously using a polarization-resolved imager. The imaging technique presented here quantitatively reports the rotational mobility of a fluorophore as it varies according to the local environment. In a single acquisition run it yields maps of both rotational correlation time and fluorescence lifetime as they vary across a sample. TR-FAIM has been applied to imaging standard multiwell plate samples of rhodamine 6G dissolved in methanol, ethylene glycol, trimethylene glycol, and glycerol. The observed rotational correlation times and fluorescence lifetimes, which report the local viscosity and refractive index of the local rhodamine 6G environment, respectively, are in good agreement with previously published single point measurements. By considering the linear dependence of the rotational correlation time on viscosity up to 20 cP, we are able to obtain a two-dimensional viscosity map. Wide-field maps of rotational correlation time, and therefore viscosity, have been obtained. This illustrates the potential to image the local viscosity and fluorescence lifetime distributions of fluorophore tagged proteins in cells.

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“…The excitation pulse duration of 7 ns was longer than the luminescence lifetime and limits the population inversion (Magde et al, 1999). Inhibition of the R6G lasing clearly indicates an energy transfer between Rh6G and SRh.…”

Section: Förster Energy Transfermentioning

confidence: 99%

Optical Characterization and Lasing in Three-Dimensional Opal-Structures

Nishijima

Juodkazis

2015

Front. Mater.

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The lasing properties of dye-permeated opal pyramidal structures are compared with the lasing properties of opal films. The opal-structures studied were made by sedimentation of microspheres and by sol-gel inversion of the direct-opals. Forced-sedimentation by centrifugation inside wet-etched pyramidal pits on silicon surfaces was used to improve the structural quality of the direct-opal structures. Single crystalline pyramids with the base length of~100 µm were formed by centrifuged sedimentation. The lasing of dyes in the well-ordered crystalline and poly-crystalline structures showed a distinct multi-modal spectrum. Gain via a distributed feedback was responsible for the lasing since the photonic band gap was negligible in a low refractive index contrast medium; the indices of silica and ethylene glycol are 1.46 and 1.42, respectively. A disordered lasing spectrum was observed from opal films with structural defects and multi-domain regions. The three-dimensional structural quality of the structures was assessed by in situ optical diffraction and confocal fluorescence. A correlation between the lasing spectrum and the three-dimensional structural quality was established. Lasing threshold of a sulforhodamine dye in a silica opal was controlled via Förster mechanism by addition of a donor rhodamine 6G dye. The lasing spectrum had a well-ordered modal structure, which was spectrally stable at different excitation powers. The sharp lasing threshold characterized by a spontaneous emission coupling ratio, β ≃ 10 −2 , was obtained.

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Solvent Dependence of the Fluorescence Lifetimes of Xanthene Dyes

Cited by 479 publications

References 57 publications

Microviscosity of Supercooled Water Confined within Aminopropyl-modified Mesoporous Silica as Studied by Time-resolved Fluorescence Spectroscopy

Microviscosity of Supercooled Water Confined within Aminopropyl-modified Mesoporous Silica as Studied by Time-resolved Fluorescence Spectroscopy

Wide-field time-resolved fluorescence anisotropy imaging (TR-FAIM): Imaging the rotational mobility of a fluorophore

Optical Characterization and Lasing in Three-Dimensional Opal-Structures

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