Solvent effects on composition and structure of thiolipid molecular anchors for tethering phospholipid bilayers

Tumėnas, Saulius; Ragaliauskas, Tadas; Penkauskas, Tadas; Valančiūtė, Audronė; Valinčius, Gintaras

doi:10.1016/j.apsusc.2020.145268

Cited by 11 publications

(8 citation statements)

References 48 publications

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“…In particular, we found that the defectiveness of tBLMs increases in the following sequence of backfillers: 3M1P, 4M1B, 6M1H, and 9M1N. We related such effect to a variation in the surface concentration of backfillers due to their different hydrophobicity, an effect analogous to the one described earlier [36]. Due to the high flexibility of anchor molecules, the WC14 alkane chains can possibly interact with backfiller molecules, especially the longer ones, which may also be the reason for the higher tBLM defectiveness.…”

Section: Discussionsupporting

confidence: 64%

“…Such a variation may be induced by variation in hydrophobicity of the backfillers from relatively low in case of 3M1P to high in case of 9M1N. The increasing hydrophobicity caused the increase in the chemical potential of backfiller, thus facilitating more hydrophobic components to form bonds on the silver surfaces, as was observed in similar experiments on gold [36]. Consequently, the surface concentration of 9M1N was likely higher compared to the one of 3M1P causing an increased defectiveness in case of the former.…”

Section: Eis Analysis Of Tblmsmentioning

confidence: 87%

“…Such data suggest that despite significantly higher defectiveness of tBLM the sensitivity to pore-forming proteins can still be observed for tBLMs assembled using longer chain backfillers. Because the concentration of SAM components on the surface may differ from the one in SAM forming solution [36] it is conceivable that the increase in the backfiller molecular chain length should be followed by the decrease in WC14 concentration. Such action would decrease the surface density of anchoring molecules WC14 thus providing worse insulating properties of tBLMs.…”

Section: A Biological Relevance Of Tblm On Silver Substrates Containing Different Short-chain Backfiller Moleculesmentioning

confidence: 99%

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The Impact of an Anchoring Layer on the Formation of Tethered Bilayer Lipid Membranes on Silver Substrates

et al. 2021

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Tethered bilayer lipid membranes (tBLMs) have been known as stable and versatile experimental platforms for protein–membrane interaction studies. In this work, the assembly of functional tBLMs on silver substrates and the effect of the molecular chain-length of backfiller molecules on their properties were investigated. The following backfillers 3-mercapto-1-propanol (3M1P), 4-mercapto-1-butanol (4M1B), 6-mercapto-1-hexanol (6M1H), and 9-mercapto-1-nonanol (9M1N) mixed with the molecular anchor WC14 (20-tetradecyloxy-3,6,9,12,15,18,22 heptaoxahexatricontane-1-thiol) were used to form self-assembled monolayers (SAMs) on silver, which influenced a fusion of multilamellar vesicles and the formation of tBLMs. Spectroscopic analysis by SERS and RAIRS has shown that by using different-length backfiller molecules, it is possible to control WC14 anchor molecules orientation on the surface. An introduction of increasingly longer surface backfillers in the mixed SAM may be related to the increasing SAMs molecular order and more vertical orientation of WC14 at both the hydrophilic ethylenoxide segment and the hydrophobic lipid bilayer anchoring alkane chains. Since no clustering of WC14 alkane chains, which is deleterious for tBLM integrity, was observed on dry samples, the suitability of mixed-component SAMs for subsequent tBLM formation was further interrogated by electrochemical impedance spectroscopy (EIS). EIS showed the arrangement of well-insulating tBLMs if 3M1P was used as a backfiller. An increase in the length of the backfiller led to increased defectiveness of tBLMs. Despite variable defectiveness, all tBLMs responded to the pore-forming cholesterol-dependent cytolysin, vaginolysin in a manner consistent with the functional reconstitution of the toxin into phospholipid bilayer. This experiment demonstrates the biological relevance of tBLMs assembled on silver surfaces and indicates their utility as biosensing elements for the detection of pore-forming toxins in liquid samples.

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Section: Discussionsupporting

confidence: 64%

Section: Eis Analysis Of Tblmsmentioning

confidence: 87%

Section: A Biological Relevance Of Tblm On Silver Substrates Containing Different Short-chain Backfiller Moleculesmentioning

confidence: 99%

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The Impact of an Anchoring Layer on the Formation of Tethered Bilayer Lipid Membranes on Silver Substrates

et al. 2021

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“…However, homogeneous assembling of a highly oriented monolayer by the wet process is difficult because of (a) the hydrolysis of a self-assembled molecule by itself and (b) its chemical bonding with either a substrate or an adjacent self-assembled molecule involves a wide variety of organic functional moieties. Moreover, the growth of a highly ordered SAM using a wet process is quite tricky, involving many parameters, such as the type of solvents used, − the presence of trace amounts of water during silanization, the soaking time and temperature of silanization, , and the baking condition after the silanizations. , For example, water exists in an environment at all times with changeable partial pressures depending on humidity levels. Therefore, it has to be critically controlled during wet growth of SAMs because excess (or varying) water content could cause uncontrolled hydrolysis and polymerization of R–Si–X 3 molecules on the surface and/or the formation of oligomers and polymers of silane in situ in a solution of the molecule to be self-assembled .…”

Section: Introductionmentioning

confidence: 99%

Chemical-Structure Evolution Model for the Self-Assembling of Amine-Terminated Monolayers on Nanoporous Carbon-Doped Organosilicate in Tightly Controlled Environments

et al. 2020

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Amine-terminated self-assembled monolayers are molecular nanolayers, typically formed via wet-chemical solution on specific substrates for precision surface engineering or interface modification. However, homogeneous assembling of a highly ordered monolayer by the facile, wet method is rather tricky because it involves process parameters, such as solvent type, molecular concentration, soaking time and temperature, and humidity level. Here, we select 3-aminopropyltrimethoxysilane (APTMS) as a model molecule of aminosilane for the silanization of nanoporous carbon-doped organosilicate (p-SiOCH) under tightly controlled process environments. Surface mean roughness (R a) and the water contact angle (θ) of the p-SiOCH layers upon silanization at a 10% humidity-controlled environment behave similarly and follow a three-stage evolution: a leap to a maximum at 15 min for R a (from 0.227 to 0.411 nm) and θ (from 25 to 86°), followed by a gradual decrease to 0.225 nm and 69o, finally leveling off at the above values (>60 min). The −NH3 + fraction indicating monolayer disorientation evolves in a similar fashion. The fully grown monolayer is highly oriented yielding an unprecedented low −NH3 + fraction of 0.08 (and 0.92 of upright −NH2 groups). However, while having a similar thickness of approximately 1.4 ± 0.1 nm, the molecular layers grown at 30% relative humidity exhibit a significantly elevated −NH3 + fraction of 0.42, indicating that controlling the humidity is vital to the fabrication of highly oriented APTMS molecular layers. A bonding-structure evolution model, as distinct from those offered previously, is proposed and discussed.

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“…Thus, hygroscopic and lipolytic agents seem to be able to disrupt the SARS-CoV-2 envelope (13, 14). Such compounds denature proteins structure by absorbing excess water and include (i) Organic solvents such as ethanol, ether, chloroform and DMSO (ii) Ionic and non-ionic detergents such as Tween 80, Triton X100 and SDS (15).…”

Section: Introductionmentioning

confidence: 99%

Application of nasal spray containing dimethyl sulfoxide (DMSO) and ethanol during the COVID-19 pandemic may protect healthcare workers: A randomized controlled trials

Hosseinzadeh

Tavakkolian

Kia

et al. 2021

Preprint

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BackgroundCoronavirus pandemic has affected a large population worldwide. Currently, the standard care for individuals who are exposed is supportive care, symptomatic management, and isolation. The aim of our study was to evaluate effects of combined use of ethanol and DMSO as a nasal spray in preventing COVID-19.MethodsWe conducted a randomized controlled trial on volunteer healthcare workers of medical centers that were at the forefront of the fight against COVID-19 in Shahroud, Iran. Two hundred and thirty-two participants were randomly assigned to intervention and control groups to receive DSMO/ethanol or routine care, respectively. The subjects were followed for 4 weeks to determine the incidence of COVID-19 infection in each group based on the RT-qPCR test. Finally, absolute risk difference and relative risk were calculated to evaluate the effect of DSMO in prevent COVID-19.ResultsThe results showed that the incidence of COVID-19 in the control group and intervention group were 0.07 and 0.008, respectively. The relative risk (RR) was 0.12 (0.9-0.02) according to the incidence rate in the two groups.Conclusioncombined application of DSMO and ethanol in healthcare providers can considerably prevent COVID-19.

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Solvent effects on composition and structure of thiolipid molecular anchors for tethering phospholipid bilayers

Cited by 11 publications

References 48 publications

The Impact of an Anchoring Layer on the Formation of Tethered Bilayer Lipid Membranes on Silver Substrates

The Impact of an Anchoring Layer on the Formation of Tethered Bilayer Lipid Membranes on Silver Substrates

Chemical-Structure Evolution Model for the Self-Assembling of Amine-Terminated Monolayers on Nanoporous Carbon-Doped Organosilicate in Tightly Controlled Environments

Application of nasal spray containing dimethyl sulfoxide (DMSO) and ethanol during the COVID-19 pandemic may protect healthcare workers: A randomized controlled trials

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