Solvent optimization for efficient enzymatic monoacylglycerol production based on a glycerolysis reaction

Damstrup, Marianne; Jensen, Thomas; Sparsø, Flemming Vang; Kiil, Søren; Jensen, Anders B.; Xu, Xinyue

doi:10.1007/s11746-005-1109-y

Cited by 102 publications

(101 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Damstrup et al (Damstrup, Jensen, Sparsø, Kiil, Jensen, & Xu, 2005) evaluated various solvents and concluded that the highest MAG yield by glycerolysis were obtained with tertiary alcohols, tert-butanol (TB) and tert-pentanol (TP). The relatively low log P value of these tertiary alcohols (0.35 and 0.89, respectively) indicates both hydrophilic and hydrophobic characteristics, ensuring miscibility of the reactants.…”

Section: Introductionmentioning

confidence: 99%

Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

et al. 2016

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

et al. 2016

View full text Add to dashboard Cite

“…5. Glyceryle monooleaate has many uses including preventing cardiovascular disorders by donating unsaturated fatty acids [38,43,44]. 6.…”

Section: Uses and Applications Of Glyceridesmentioning

confidence: 99%

Progress in Synthesis of Monoglycerides for Use in Food and Pharmaceuticals

et al. 2017

View full text Add to dashboard Cite

Glycerides are lipid esters of the glycerol molecule and fatty acids. Their primary function is the storage of energy. Due to its structure and properties, glycerol participates in the formulation or synthesis of many compounds such as food products, cosmetics, pharmaceuticals, liquid detergents. Monoglycerides (MGs) can be formed by both industrial chemical glycerolysis and biological or enzymatic processes. Chemical glycerolysis bring issues of low MGs yield, high operating temperature, formation of undesirable by-products and high energy consumption. On the other hand enzymatic processes have advantages of mild reaction conditions and high purity of MGs. But, several purification steps are required to obtain food or pharmaceutical grade MG, such as neutralization of the reaction media and discoloration followed by expensive molecular distillation. The purpose of this article is to review the main challenges in the synthesis of MGs from triglycerides (TGs) contained in the various fixed oils and application thereof in the food and pharmaceuticals.

show abstract

“…According to Damstrup et al (2005), tert-butanol and tert-pentanol resulted in excellent solvents to carry out these reactions. Their log P values (0.35 and 0.89, respectively) indicate both hydrophilic and hydrophobic characteristics.…”

Section: Introductionmentioning

confidence: 99%

Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

Solaesa¹,

Sanz²,

Melgosa³

et al. 2015

Grasas y Aceites

View full text Add to dashboard Cite

SUMMARY:The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA) via enzymatic glycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two different tert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneous system has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IM from Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameters were studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant mole ratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (based on weight of reactants), achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fatty acid content (lower than 18 wt%).KEYWORDS: Fish oil; Glycerolysis; Lipase; Monoacylglycerol; Rhizomucor miehei; Tert-alcohols RESUMEN: Glicerolisis de aceite de sardina catalizada por una lipasa dependiente de agua en diferentes tert-alcoholes como medio de reacción. En este trabajo se ha estudiado la producción de monoacilglicéridos, ricos en ácidos grasos poliinsaturados (AGPI), mediante glicerolisis enzimática de aceite de sardina. La reacción se ha llevado a cabo en dos tert-alcoholes para conseguir de esta forma un medio homogéneo de reacción. La cantidad de disolvente añadida al medio de reacción se ha optimizado y calculado en base al equilibrio de fases de los componentes del sistema. La lipasa empleada como biocatalizador ha sido la enzima inmovilizada Lipozyme RM IM de Rhizomucor miehei, una lipasa dependiente de agua. Se ha estudiado el efecto de distintos parámetros cinéticos, así como de la cantidad de agua añadida al medio de reacción, en la producción de monoacilglicéridos. De los resultados obtenidos, se puede concluir que, para una relación molar inicial de reactantes glicerol:aceite de sardina de 3:1, un 12 % en peso de agua en base al glicerol y un 10 % en peso de lipasa, en base al peso de reactantes; se puede llegar a conseguir un rendimiento en monoacilglicéridos alrededor del 70 % en peso, con casi un 28 % en peso de AGPI y un bajo contenido en ácidos grasos libres (menor del 18 % en peso).

show abstract

Solvent optimization for efficient enzymatic monoacylglycerol production based on a glycerolysis reaction

Cited by 102 publications

References 21 publications

Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

Progress in Synthesis of Monoglycerides for Use in Food and Pharmaceuticals

Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

Contact Info

Product

Resources

About