Solvent-tolerant bacteria for biotransformations in two-phase fermentation systems

Heipieper, Hermann J.; Neumann, Grit; Cornelissen, Sjef; Meinhardt, Friedhelm

doi:10.1007/s00253-006-0833-4

Cited by 306 publications

(256 citation statements)

References 85 publications

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“…Until now, bacterial solvent tolerance was studied mainly with aerobic, biotechnologically relevant microorganisms. The solvent stress response of these organisms involved mainly solvent efflux pumps, heat shock proteins, and modifications of the cytoplasmic membrane (19,22). Here, we investigated for the first time the physiological and proteomic responses of the denitrifying bacterium "Aromatoleum aromaticum" strain EbN1 to semiinhibitory concentrations of its aromatic growth substrates.…”

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confidence: 99%

Solvent Stress Response of the Denitrifying Bacterium “ Aromatoleum aromaticum ” Strain EbN1

Trautwein

Kühner

Wöhlbrand

et al. 2008

Appl Environ Microbiol

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The denitrifying betaproteobacterium "Aromatoleum aromaticum" strain EbN1 degrades several aromatic compounds, including ethylbenzene, toluene, p-cresol, and phenol, under anoxic conditions. The hydrophobicity of these aromatic solvents determines their toxic properties. Here, we investigated the response of strain EbN1 to aromatic substrates at semi-inhibitory (about 50% growth inhibition) concentrations under two different conditions: first, during anaerobic growth with ethylbenzene (0.32 mM) or toluene (0.74 mM); and second, when anaerobic succinate-utilizing cultures were shocked with ethylbenzene (0.5 mM), toluene (1.2 mM), p-cresol (3.0 mM), and phenol (6.5 mM) as single stressors or as a mixture (total solvent concentration, 2.7 mM). Under all tested conditions impaired growth was paralleled by decelerated nitrate-nitrite consumption. Additionally, alkylbenzene-utilizing cultures accumulated poly(3-hydroxybutyrate) (PHB) up to 10% of the cell dry weight. These physiological responses were also reflected on the proteomic level (as determined by two-dimensional difference gel electrophoresis), e.g., up-regulation of PHB granule-associated phasins, cytochrome cd 1 nitrite reductase of denitrification, and several proteins involved in oxidative (e.g., SodB) and general (e.g., ClpB) stress responses.

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confidence: 99%

Solvent Stress Response of the Denitrifying Bacterium “ Aromatoleum aromaticum ” Strain EbN1

Trautwein

Kühner

Wöhlbrand

et al. 2008

Appl Environ Microbiol

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“…Owing to this, the bacterial membranes become more resistant to the fluidizing action of aromatic compounds, which allows the cells to survive in the contaminated sites (Salar et al 2014). The other adaptive mechanisms might involve changes in the following: membrane protein pattern, energy metabolism, cell morphology, the cell wall as well as the vesicle formation and transformation or degradation of xenobiotics (Heipieper et al 2007;Schrewe et al 2013). The higher degradation of SMX and DCF in the presence of phenol could also be explained by co-metabolic interaction.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

Węgrzyn

Felis

2018

Polish Journal of Microbiology

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A b s t r a c t Diclofenac (DCF), a non-steroidal anti-inflammatory drug (NSAID) and sulfamethoxazole (SMX), an antimicrobial agent, are in common use and can be often detected in the environment. The constructed wetland systems (CWs) are one of the technologies to remove them from the aquatic environment. The final effect of the treatment processes depends on many factors, including the interaction between plants and the plant-associated microorganisms present in the system. Bacteria living inside the plant as endophytes are exposed to secondary metabolites in the tissues. Therefore, they can possess the potential to degrade aromatic structures, including residues of pharmaceuticals. The endophytic strain MG7 identified as Microbacterium sp., obtained from root tissues of Phalaris arundinacea exposed to DCF and SMX was tested for the ability to remove 2 mg/l of SMX and DCF in monosubstrate cultures and in the presence of phenol as an additional carbon source. The MG7 strain was able to remove approximately 15% of DCF and 9% of SMX after 20 days of monosubstrate culture. However, a decrease in the optical density of the MG7 strain cultures was observed, caused by an insufficient carbon source for bacterial growth and proliferation. The adsorption of pharmaceuticals onto autoclaved cells was negligible, which confirmed that the tested strain was directly involved in the removal of DCF and SMX. In the presence of phenol as the additional carbon source, the MG7 strain was able to remove approximately 35% of DCF and 61% of SMX, while an increase in the optical density of the cultures was noted. The higher removal efficiency can be explained by adaptive mechanisms in microorganisms exposed to phenol (i.e. changes in the composition of membrane lipids) and by a co-metabolic mechanism, where non-growth substrates can be transformed by non-specific enzymes. The presence of both DCF and SMX and the influence of the supply frequency of CWs with the contaminated wastewater on the diversity of whole endophytic bacterial communities were demonstrated. The results of this study suggest the capability of the MG7 strain to degrade DCF and SMX. This finding deserves further investigations to improve wastewater treatment in CWs with the possible use of pharmaceuticals-degrading endophytes.K e y w o r d s: endophytic bacteria, constructed wetlands, diclofenac, sulfamethoxazole, biodegradation Węgrzyn A. and Felis E.

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“…The TPPB systems have been used as a platform for treatment of environmental pollutants, fermentations as well as biocatalysis (Deziel et al 1999;Daugulis 2001;Malinowski 2001;Schmid et al 2001;Heipieper et al 2007;Muñoz et al 2007). By and large, the TPPB systems offer the prospect of increased mass transfer, alleviation of possible substrate and product toxicity, and hence, enhanced biotransformations and simplified product recovery.…”

Section: Introductionmentioning

confidence: 99%

Bioproduction of lauryl lactone and 4-vinyl guaiacol as value-added chemicals in two-phase biotransformation systems

Yang

Wang

Lorrain

et al. 2009

Appl Microbiol Biotechnol

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Recombinant Escherichia coli whole-cell biocatalysts harboring either a Baeyer-Villiger monooxygenase or ferulic acid decarboxylase were employed in organic-aqueous two-phase bioreactor systems. The feasibility of the bioproduction of water-insoluble products, viz., lauryl lactone from cyclododecanone and 4-vinyl guaiacol from ferulic acid were examined. Using hexadecane as the organic phase, 10∼16 g of lauryl lactone were produced in a 3-l bioreactor that operated in a semicontinuous mode compared to 2.4 g of product in a batch mode. For the decarboxylation of ferulic acid, a new recombinant biocatalyst, ferulic acid decarboxylase derived from Bacillus pumilus, was constructed. Selected solvents as well as other parameters for in situ recovery of vinyl guaiacol were investigated. Up to 13.8 g vinyl guaiacol (purity of 98.4%) were obtained from 25 g of ferulic acid in a 2-l working volume bioreactor by using octane as organic phase. These selected examples highlight the superiority of the two-phase biotransformations systems over the conventional batch mode.

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Solvent-tolerant bacteria for biotransformations in two-phase fermentation systems

Cited by 306 publications

References 85 publications

Solvent Stress Response of the Denitrifying Bacterium “ Aromatoleum aromaticum ” Strain EbN1

Solvent Stress Response of the Denitrifying Bacterium “ Aromatoleum aromaticum ” Strain EbN1

Isolation of Bacterial Endophytes from Phalaris arundinacea and their Potential in Diclofenac and Sulfamethoxazole Degradation

Bioproduction of lauryl lactone and 4-vinyl guaiacol as value-added chemicals in two-phase biotransformation systems

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