Somaclonal variation for genetic improvement in grasspea (&lt;italic&gt;Lathyrus sativus&lt;/italic&gt; L.)

Tripathy, Swapan K.; Panda, A.; Nayak, Padma L.; Dash, SK; Lenka, Devraj; Mishra, Dayanidhi; Kar, Rajesh Kumar; Senapati, N.; Dash, G. B.

doi:10.18805/lr.v0iof.6853

Cited by 6 publications

(7 citation statements)

References 25 publications

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“…The result indicated that under the moisture stress condition maximum hundred seed weight was observed from the somaclonal plants whereas under non-stress condition maximum hundred seed weight was obtain from the parental genotype. This was in agreement with the result of Tripathy et al [26] in grass pea who reported better performance of the somaclones for hundred seed weight. On the contrary, Bouiamrine et al [19] reported hundred seed weight of somaclonal plants did not show better performance compared to the parental genotypes in durum wheat.…”

Section: Evaluation Of Somaclones (R1 Generation) and Their Respectivsupporting

confidence: 93%

“…The differences among the means of the somaclones and their respective parental genotypes for the studied parameters were highly significant (P ≤ 0.01) for both experimental conditions (Table 2). In line with the present results, significant differences through the in vitro regenerated plants and the donor parents have been reported previously in many crops [19,[24][25][26].…”

Section: Evaluation Of Somaclones (R1 Generation) and Their Respectivsupporting

confidence: 91%

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Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Ferede¹,

Mekbib²,

Assefa³

et al. 2018

Adv Crop Sci Tech

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Breeding for moisture stress tolerance by conventional methods is not satisfactory in view of current demand to increase crop productivity. Inducing somaclonal variation is a potential technique to obtain stress tolerant variants. The present study was conducted to evaluate the performance of tef somaclones (R1 generation) and their respective parental genotypes for drought tolerance. The materials used in this experiment were obtained from the R0 generation of previous in vitro culture experiment. Twelve seed derived somaclones; Melko 0, Melko 0.5, Melko 1, Melko 1.5, Gemechis 0, Gemechis 0.5, Gemechis 1, Gemechis 1.5, Pop12S20, Pop12S20.5, Pop12S21 and Pop12S21.5 and their respective parental genotypes (Melko, Gemechis and Pop12S2) were used under two set of experiments (i.e., under moisture stress and non-stress conditions) in pots under green house. The experiments were laid out in completely randomized design with three replications. Analysis of variance revealed that somaclones (R1 generation) and their parental genotypes showed highly significant (P ≤ 0.01) difference in all the measured traits under both moisture regimes. Comparisons were made between the somaclones and their parents under moisture stress and non-stress conditions for the measured traits. Under moisture stress condition superiority of the somaclones was observed except for number of spikelet/panicle and total number of tiller/plant. On the other hand, under non-stress condition better performance was observed from parental genotypes for number of spikelet/ panicle, panicle weight, panicle seed weight, plant weight, plant seed weight and grain yield. The results showed that all the somaclones and their parental genotypes were negatively affected by moisture stress for all studied parameters. However, somaclones were less affected by moisture stress as compared to the parental genotypes. Hence, in vitro culture induced somaclonal variation can effectively be used for selection and improvement of drought tolerant tef genotypes.

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Section: Evaluation Of Somaclones (R1 Generation) and Their Respectivsupporting

confidence: 93%

Section: Evaluation Of Somaclones (R1 Generation) and Their Respectivsupporting

confidence: 91%

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Ferede¹,

Mekbib²,

Assefa³

et al. 2018

Adv Crop Sci Tech

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“…Taxonomic relationship studies have shown that only two species, L. amphicarpos and L. cicera, produce viable hybrids when crossed with L. sativus (Yunus and Jackson 1991). Yet, to overcome strong reproductive barriers among different species, biotechnological tools, including tissue culture, somaclonal variation, and protoplast fusion techniques may also be utilized (Ochatt et al 2001;Piwowarczyk and Pindel 2015;Tripathy et al 2016).…”

Section: Variety Amelioration Researchmentioning

confidence: 99%

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Lambein

Travella

Kuo

et al. 2019

Planta

145

127

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Main conclusion Although grass pea is an environmentally successful robust legume with major traits of interest for food and nutrition security, the genetic potential of this orphan crop has long been neglected. Grass pea (Lathyrus sativus L.) is a Neolithic plant that has survived millennia of cultivation and has spread over three continents. It is a robust legume crop that is considered one of the most resilient to climate changes and to be survival food during drought-triggered famines. The hardy penetrating root system allows the cultivation of grass pea in various soil types, including marginal ones. As an efficient nitrogen fixer, it meets its own nitrogen requirements and positively benefits subsequent crops. However, already in ancient India and Greece, overconsumption of the seeds and a crippling neurological disorder, later coined neurolathyrism, had been linked. Overemphasis of their suspected toxic properties has led to disregard the plant's exceptionally positive agronomic properties and dietary advantages. In normal socioeconomic and environmental situations, in which grass pea is part of a balanced diet, neurolathyrism is virtually non-existent. The etiology of neurolathyrism has been oversimplified and the deficiency in methionine in the diet has been overlooked. In view of the global climate change, this very adaptable and nutritious orphan crop deserves more attention. Grass pea can become a wonder crop if the double stigma on its reputation as a toxic plant and as food of the poor can be disregarded. Additionally, recent research has exposed the potential of grass pea as a health-promoting nutraceutical. Development of varieties with an improved balance in essential amino acids and diet may be relevant to enhance the nutritional value without jeopardizing the multiple stress tolerance of this promising crop.

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“…Among the different explants of grass pea, the epicotyl was reported to be the most responsive with high shoot proliferation frequency. Development of somaclones in L. sativus is limited (Santha and Mehta, 2001 ; Tripathy et al, 2016 ; Barpete et al, 2020a ), but in India, such efforts have developed somaclones derived low-ODAP variety (Ratan) (Mehta et al, 1994 ; Santha and Mehta, 2001 ). Tripathy et al ( 2016 ) have also developed a series of somaclones for morphological, cytological (genetic) variation, and biochemical levels in four grass pea (Nirmal, P 24, Nayagarh local, and Dhenkanal local) genotypes.…”

Section: Breeding Approaches For Cultivar Developmentmentioning

confidence: 99%

“…A high-yielding and low ODAP somaclone (cv. NGOG 5) was developed that may be the potential candidate for future grass pea breeding programs (Tripathy et al, 2016 ). Ochatt et al ( 2002 ) and Barpete et al ( 2020a ) developed an in vitro system for shortening the generation cycles and hastening the breeding process coupled with the in vitro stages that provide up to 4–5 cycles per year of grass pea.…”

Section: Breeding Approaches For Cultivar Developmentmentioning

confidence: 99%

Current Perspectives on Reducing the β-ODAP Content and Improving Potential Agronomic Traits in Grass Pea (Lathyrus sativus L.)

Das

Parihar

Barpete³

et al. 2021

Front. Plant Sci.

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Grass pea is well-established as one of the most resilient and versatile crops that can thrive under extreme climatic circumstances such as cold, heat, drought, salt-affected soils, submergence, and excessive rainfall along with resistance to several diseases and pests. However, despite the awareness of its virtues, its cultivation globally has decreased recently owing to the presence of a neurotoxin, β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP), in the seedlings and seeds of this legume, which has been reported to cause neurolathyrism, a non-reversible neurological disorder in humans and animals. Significant repositories of Lathyrus germplasm are available across countries that have provided access to a wide range of agro-morphological traits as well as the low β ODAP content. Efforts have been made worldwide to use these germplasms for the genetic enhancement of grass pea to make this food safe for human consumption. Efforts on molecular breeding of this crop are also lagging. However, during the last decade, the research scenario has changed with some efforts being made toward improving this climate resilient pulse in terms of genomic resources. Molecular markers have also been used to evaluate the interspecific diversity as well as the phylogenetic relationship among the species and mapping studies. Intron-targeted amplified polymorphic, genomic simple sequence repeat, resistance genes analogs, and disease resistance markers developed for other legume species have been successfully cross-amplified in grass pea. Transcriptomic studies have recently been undertaken on grass pea by deploying several second-generation sequencing techniques. In addition, a few studies have attempted to unveil the genes and the underlying mechanism conferring biotic and abiotic stress or regulating the pathway of β-ODAP in grass pea. Proteomics has accelerated the identification studies on differential proteomes in response to salinity and low-temperature stress conditions for unveiling the common signaling pathways involved in mitigating these abiotic stresses and in discovering differentially regulated proteins. In grass pea, a metabolomics approach has been used to identify the metabolic processes associated with β-ODAP synthesis. Genome sequencing of grass pea is under way which is expected to be vital for whole-genome re-sequencing and gene annotation toward the identification of genes with novel functions. Recently, a draft genome sequence of grass pea was developed, and some efforts are underway to re-sequence a diverse panel of grass pea comprising 384 germplasm lines. Owing to the scantiness of a successful transformation protocol, research on the application of modern approaches of genome editing like the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) or CRISPR-associated protein 9 (CRISPR/Cas9) system for the engineering of signaling pathways or regulatory mechanisms seeks immediate attention to reduce the β-ODAP content in seeds and to improve the potential agronomic traits in grass pea.

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Somaclonal variation for genetic improvement in grasspea (<italic>Lathyrus sativus</italic> L.)

Cited by 6 publications

References 25 publications

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Current Perspectives on Reducing the β-ODAP Content and Improving Potential Agronomic Traits in Grass Pea (Lathyrus sativus L.)

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