Somaclonal variation for resistance to Verticillium dahliae in potato (Solanum tuberosum L.) plants regenerated from callus

Sebastiani, L.; Lenzi, Anna; Pugliesi, Claudio; Fambrini, Marco

doi:10.1007/bf00039292

Cited by 23 publications

(12 citation statements)

References 35 publications

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“…It has been proposed that genomic variability in transgenic plants may result from (1) tissue cultureinduced mutagenesis (Dolezel and Novak 1984;Sebastiani et al 1994); (2) pre-existing genomic variability in the original plant population, as documented in crosspollinating (Palilov et al 1981;Pooran and Singh 2003;Pradeep and sumalini 2003), self-pollinating (Miyashita et al 1999;Bered et al 2002) and cloned plants (Sala et al 2000); and (3) insertion mutagenesis, whereby the foreign gene may disrupt the gene it is inserted into or close to (Koncz et al 1992;Jong-Seong et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

et al. 2004

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The occurrence of DNA modification is an undesired phenomenon accompanying plant cell transformation. The event has been correlated with the stress imposed by the presently utilised transformation procedures, all depending on plant differentiation from in vitro cell culture, but other causes have not been excluded. In this work, transgenic Arabidopsis thaliana plants have been produced by an approach that does not require cell dedifferentiation, being based on in planta Agrobacterium-mediated gene transfer by flower infiltration, which is followed by recovery and selection of transgenic progeny. Genomic DNA changes in transgenic and control plants have been investigated by AFLP and RAMP analysis. Results show no statistically relevant genomic modifications in transgenic plants, as compared with control untreated plants. Variations were observed in callus-derived A. thaliana plants, thus supporting the conclusion that somaclonal variation is essentially correlated with the stress imposed by the in vitro cell culture, rather than with the integration of a foreign gene.

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Section: Discussionmentioning

confidence: 99%

Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

et al. 2004

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“…Field evaluation of 'Desiree' clones that produced adventitiously on callused shoot explants varied in tuber characteristics ). One out of 325 calliclones (0.3%) of 'Desiree' showed resistance to Verticillium dahlia in a growth chamber evaluation (Sebastiani et al 1994). Field-evaluated calliclones from nodal and internodal segments of 'Multa' and 'Diamant' showed variation in plant height, number of leaves, and yield components (Nasrin et al 2003).…”

mentioning

confidence: 96%

Intraclonal Selection for Improved Processing of NB ‘Russet Burbank’ Potato

et al. 2011

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Russet Burbank' has limited fertility and has not parented improved cultivars through traditional breeding efforts. This study showed that 'Russet Burbank' (NB clone) could be improved through selection of intraclones (somatic embryos derived from specific tuber tissues) based on field performance and/or processing characteristics. In 2005 and 2006, approx. 800 intraclones were regenerated from field-grown tubers or microtubers. Intraclones were micropropagated, acclimatized, and field-tested to identify the highest yielding lines. Each season, following storage, tubers of selected lines were tested for glucose content and French fry-processing quality. In 2007, the best intraclones from earlier seasons were increased through micropropagation and retested for yield and processing features. Approx. 2-9% of intraclones had similar yield to controls but superior processing features. Neither tuber source nor explant tissue type affected intraclone tuber yield, type, or processing characters. We recommend the incorporation of somatic embryogenesis into potato improvement programs for processing quality traits.Resumen "Russet Burbank" tiene fertilidad limitada y no ha sido progenitor que haya mejorado variedades a través de esfuerzos de mejoramiento tradicional. Este estudio demostró que "Ruset Burbank" (clon NB) pudo mejorarse por selección de intraclones (embriones somáticos derivados de tejidos específicos de tubérculo) con base en el comportamiento en el campo y/o en características de procesamiento. En 2005 y 2006, se regeneraron aproximadamente 800 intraclones de tubérculos cultivados en el campo o de microtubérculos. Los intraclones se micropropagaron, se aclimataron, y se probaron en el campo para identificar las líneas de rendimiento más alto. En cada ciclo de cultivo, después del almacenamiento, se probaron tubérculos de líneas selectas para el contenido de glucosa y de calidad de fritura para papas a la francesa. En 2007, los mejores intraclones de siembras previas se incrementaron por micropropagación y se volvieron a probar para rendimiento y características de procesamiento. Aproximadamente el 2-9% de intraclones tuvieron rendimiento similar al de los testigos pero mejores características de procesamiento. Ni la fuente del tubér-culo ni el tipo de tejido del explante (inóculo) afectaron el rendimiento de tubérculo de los intraclones, o el tipo o características del procesamiento. Recomendamos la incorporación de embriogénesis somática en los programas de mejoramiento de papa para las características de calidad en el procesamiento.

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“…Taylor and Secor (1990) found differential callus longevity when protoplast-derived calluses of potato cv. Sebastiani et al (1994) selected nodal explants from shoots regenerated from stern explants of potato cv. carotovora.…”

Section: History and Application Of Somaclonal Variation In The Solanmentioning

confidence: 99%

Somaclonal Variation and Induced Mutations in Crop Improvement

Jain¹,

Brar²,

Ahloowalia³

1998

Current Plant Science and Biotechnology in Agriculture

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Aims and ScopeThe book series is intended for readers ranging from advanced students to senior research scientists and corpomte directors interested in acquiring in-depth, state-of-the-art knowledge about research findings and techniques related to all aspects of agricultural biotechnology. Although the previous volumes in the series dealt with plant science and biotechnology, the aim is now to also include volumes dealing with animals science, food science and rnicrobiology. While the subject matter will relate more particularly to agricultural applications, timely topics in basic science and biotechnology will also be explored. Some volumes will report progress in rapidly advancing disciplines through proceedings of symposia and workshops while others will detail fundamental information of an enduring nature that will be referenced repeatedly.The titles published in this series are listed at the end ofthis volume.

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Somaclonal variation for resistance to Verticillium dahliae in potato (Solanum tuberosum L.) plants regenerated from callus

Cited by 23 publications

References 35 publications

Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

Intraclonal Selection for Improved Processing of NB ‘Russet Burbank’ Potato

Somaclonal Variation and Induced Mutations in Crop Improvement

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