Somaclonal variation in embryogenic cultures of silver fir (<i>Abies alba</i>Mill.)

Nawrot-Chorabik, Katarzyna

doi:10.1080/11263500902722717

Cited by 15 publications

(8 citation statements)

References 23 publications

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“…We hypothesized that both procedures used could negatively affect the genetic stability of plant material. Several studies have reported somaclonal variation in embryonic cultures both after in vitro cultivation and after cryopreservation (Burg et al, 2007;DeVerno et al, 1999;Helmersson et al, 2008;Nawrot-Chorabik, 2009;Isabel et al, 1996;Krajňáková et al, 2011;Dey et al, 2015). In this study we report substantial genetic instability in SSR genotypes of the studied material as 52 different mutation events in total were detected.…”

Section: Discussionmentioning

confidence: 52%

Somaclonal Variation During Picea abies and P. omorika Somatic Embryogenesis and Cryopreservation

Hazubska-Przybył¹,

Dering²

2017

Acta Biologica Cracoviensia S. Botanica

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Embryogenic cultures of plants are exposed to various stress factors both in vitro and during cryostorage. In order to safely include the plant material obtained by somatic embryogenesis in combination with cryopreservation for breeding programs, it is necessary to monitor its genetic stability. The aim of the present study was the assessment of somaclonal variation in plant material obtained from embryogenic cultures of Picea abies (L.) Karst. and P. omorika (Pančić) Purk. maintained in vitro or stored in liquid nitrogen by the pregrowth-dehydration method. The analysis of genetic conformity with using microsatellite markers was performed on cotyledonary somatic embryos (CSE), germinating somatic embryos (GSE) and somatic seedlings (SS), obtained from tissues maintained in vitro or from recovered embryogenic tissues (ETc) and CSE obtained after cryopreservation. The analysis revealed changes in the DNA of somatic embryogenesis-derived plant material of both Picea spp. They were found in plant material from 8 out of 10 tested embryogenic lines of P. abies and in 10 out of 19 embryogenic lines of P. omorika after in vitro culture. Changes were also detected in plant material obtained after cryopreservation. Somaclonal variation was observed in ETc and CSE of P. omorika and at ETv stage of P. abies. However, most of the changes were induced at the stage of somatic embryogenesis initiation. These results confirm the need for monitoring the genetic stability of plants obtained by somatic embryogenesis and after cryopreservation for both spruce species.

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Section: Discussionmentioning

confidence: 52%

Somaclonal Variation During Picea abies and P. omorika Somatic Embryogenesis and Cryopreservation

Hazubska-Przybył¹,

Dering²

2017

Acta Biologica Cracoviensia S. Botanica

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“…• Selecting the best genotypes (lines) of callus, which should be proliferated until obtaining at least 15 clones of each genotype weighing 300 -500 mg each [15,16,17].…”

Section: Stages Of Dual Culturesmentioning

confidence: 99%

The Use of Interactions in Dual Cultures in vitro to Evaluate the Pathogenicity of Fungi and Susceptibility of Host Plant Genotypes

Nawrot-Chorabik¹

2013

Environmental Biotechnology - New Approaches and Prospective Applications

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“…4°C. Z nasion, pod lup¹ binokularn¹, izolowano dojrza³e zarodki zygotyczne, które wyk³adano, po 5 sztuk, do szalek Petriego na po¿ywkê SH uzupe³nion¹ regulatorami wzrostu (BA -6-benzylo-aminopuryna, KIN -kinetyna, 2,4-Dkwas dichlorofenoksyoctowy), witaminami (tiamina, pirydoksyna, kwas nikotynowy), 2,0 % sacharoz¹ i 0,4% phytogelem (Nawrot-Chorabik 2008, 2009). Hodowlê kalusa prowadzono w inkubatorze, w temperaturze 25°C, w ciemnooeci.…”

Section: Kalus Jod³yunclassified

Interactions among three genotypes of Abies alba callus and fungi with different ecological status

Nawrot-Chorabik¹,

Jankowiak²

2010

Forest Research Papers

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Interakcje pomiêdzy kalusem trzech genotypów Abies alba a grzybami o ró¿nym statusie ekologicznymInteractions among three genotypes of Abies alba callus and fungi with different ecological status Abstract. Callus cultures derived from three Abies alba genotypes were co-cultivated with several fungi of different ecological status. Growth of Heterabasidion parviporum, Ophiostoma piceae and Pesotum sp. was stimulated, whilst Heterobasidon abietinum and Geosmithia sp. were unaffected by the presence of callus (statistical analyses for these 2 fungi gave no difference and a marginally-significant different respectively).Fir callus inhibited the growth of Pesotum fragrans and Rizosphaera macrospora. Otherwise, there were significant differences between the callus genotypes with respect to the growth of fungi and colonization of fir callus. Fungi H. abietinum, O. piceae, R. macrospora and Geosmithia sp. first colonized callus belonging to genotype G6. In contrast, fungal species: P. fragrans, Pesotum sp., Geosmithia sp. and H. parviporum quickly colonized callus derived from the genotype G5. Across all genotypes, callus was colonized in the shortest period of time by the fungus H. parviporum (causing callus necrosis after 6-9 days), the slowest was Pesotum sp. (causing callus necrosis after 60-81 days). The importance of these results regarding pathogenicity and behaviour in vivo of these fungi is discussed.

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Somaclonal variation in embryogenic cultures of silver fir (Abies albaMill.)

Cited by 15 publications

References 23 publications

Somaclonal Variation During Picea abies and P. omorika Somatic Embryogenesis and Cryopreservation

Somaclonal Variation During Picea abies and P. omorika Somatic Embryogenesis and Cryopreservation

The Use of Interactions in Dual Cultures in vitro to Evaluate the Pathogenicity of Fungi and Susceptibility of Host Plant Genotypes

Interactions among three genotypes of Abies alba callus and fungi with different ecological status

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