Somaclonal Variation of Caladium Bicolor (Ait.) Vent. 'Jao Ying' After in Vitro Culture Propagation

Thongpukdee, Aree; Thepsithar, C.; Chiensil, P.

doi:10.17660/actahortic.2010.855.42

Cited by 8 publications

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“…Although there were a number of reports on the type and frequency of somaclonal variation in caladium (Ahmed et al 2002(Ahmed et al , 2004(Ahmed et al , 2007Somkanea et al 2010;Thepsithar et al 2010Thepsithar et al , 2011Thongpukdee et al 2010), little was known about somaclonal variation at the cellular and molecular levels. The objectives of this study were to (1) assess the type and frequency of somaclonal variation in a caladium cultivar that was widely grown and phenotypically stable, (2) evaluate the effect of two types of explant and auxin on somaclonal variation, and (3) characterize the nuclear DNA content, chromosome number, and molecular marker banding pattern of the obtained somaclonal variants.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Cao

Sui

Cai

et al. 2016

Plant Cell Tiss Organ Cult

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Somaclonal variation is a major concern to many applications of plant cell and tissue culture techniques, yet it could be a useful source of variation for plant genetic improvement. High frequencies of somaclonal variants were reported in caladium, but little was known about changes in caladium somaclonal variants at the cellular and molecular levels. Twenty-four somaclonal variants were identified among 'Red Flash' caladium plants regenerated from two types of leaf explants and on two media containing different auxins. Results showed that the type of leaf explants and auxin affected the occurrence of somaclonal variants in 'Red Flash'. The highest percentage of variants (25.0 %) was observed among plants regenerated from mature leaf explants cultured on the media containing 2,4-dichlorophenoxyacetic acid. These somaclonal variants exhibited considerable changes in leaf shape, coloring of the main veins, spots, margins, and leaf size, and could be separated into 10 somaclonal variant groups. Twelve variants contained 1.1-5.4 % less nuclear DNA and appeared to have lost one chromosome. Two variants contained 5.4-9.2 % less nuclear DNA and appeared to have lost two chromosomes. One variant contained 95.0 % more nuclear DNA and 2n = 58 chromosomes. Two SSR markers (CaM1 and CaM103) revealed DNA banding pattern changes in nine variants, including allele loss in eight variants and allele size change in one variant. These results suggested that several cytological and/or molecular causes were involved in the somaclonal variation in 'Red Flash' and chromosome number change was a frequent cause leading to a high frequency of aneuploids and marker allele loss. The CaM1-carrying chromosome seemed to be unstable and prone to loss during tissue culture.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Cao

Sui

Cai

et al. 2016

Plant Cell Tiss Organ Cult

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“…In caladium, callus, shoots and roots were readily induced from a range of explant types such as leaf segments on a suitable medium (Ahmed et al 2004;Thongpukdee et al 2010). In this study, callus formation was also observed when the leaf segments were cultured on the MS basal medium supplemented with 1 mg/L TDZ and 1 mg/L NAA.…”

Section: Discussionmentioning

confidence: 61%

“…Caladium (Caladium × hortulanum Birdsey) is an important ornamental plant valued for its long-lasting colorful foliage, and is commonly grown in containers and in the landscape (Deng 2018(Deng , 2012. A great deal of reports indicated that caladium could be propagated by in vitro culturing of leaf segments, meristematic tissues or petiole segments (Ahmed et al 2004;Thongpukdee et al 2010), and morphological variants were frequently observed among these regenerated plants (Cai et al 2015;Cao et al 2016). Recent studies found that chromosome gain or loss was a common type of cytological variation in caladium (Cai et al 2015;Cao et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Nutrient Solutions on the Acclimatisation of in vitro Caladium Plantlets using a Simplified Hydroponic System

Zhang¹,

Gu²,

Chen³

et al. 2019

JSM

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An efficient acclimatisation protocol is critical to improve the survival rate and promote growth of in vitro plantlets. Herein, tissue-cultured plantlets of 'Red Flash' caladium (Caladium × hortulanum Birdsey) were treated with deionised water and three nutrient solutions including Japanese garden formula (JG), South China Agriculture University formula B for leafy vegetables (SCAU-B) and Hoagland solution in a simplified hydroponic system under indoor environmental conditions. The results showed that all the plantlets survived after 30 days of hydroponic culture, and the three nutrient solutions showed significant positive effects on the plant growth and the content of chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, soluble sugar and soluble protein compared to the deionised water treatment. No significant difference was recorded for the other growth parameters except the average leaf number per plantlet among the nutrient solution treatments. For the analysed physiological parameters, the plantlets treated by SCAU-B contained significantly higher content than those of the Hoagland treatment except the protein content. No significant difference was found between the JG and the SCAU-B treatment except the carotenoid content, and between the JG and the Hoagland treatment except the protein content. The plantlets acclimatised in the nutrient solutions also had a good performance when transferred to plastic pots. These results implied that water alone was not capable of maintaining healthy growth of in vitro caladium plantlets, and hydroponics approach using the three nutrient solutions could be a feasible method for acclimatisation of the plantlets.

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“…For example, up to 80% of the regenerated plants of several caladium cultivars showed obvious phenotypic changes compared to the original stock plants (Ahmed et al 2004;Chu and Yazawa 2001). Phenotypic changes in caladium somaclonal variants seem to mainly involve leaf characters (Ahmed et al 2002(Ahmed et al , 2004Thepsithar et al 2010;Thongpukdee et al 2010). The occurrence of somaclonal variation in caladium seems to be dependent on the use of plant regulator, explant type and plant genotype.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Morphological, cytological and molecular marker analyses of ‘Tapestry’ caladium variants reveal diverse genetic changes and enable association of leaf coloration pattern loci with molecular markers

Cao

Deng

2020

Plant Cell Tiss Organ Cult

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Somaclonal variation and induced mutation are highly valuable sources of genetic variation for genetic improvement and research in horticultural crops; cellular and molecular characterization can greatly facilitate their uses in these areas. In the present study, 20 caladium variants were identified among ‘Tapestry’ plants that were regenerated from leaf cultures treated with or without colchicine. These variants showed changes in leaf main vein color and coloration pattern and were separated into ten groups based on leaf morphological changes. Five variants contained 3.3–9.7% more nuclear DNA than the wildtype and gained one, two or three chromosomes, while four variants contained 3.0–4.8% less nuclear DNA and lost one chromosome. Five, out of 22, simple sequence repeat-based molecular markers detected DNA banding pattern changes in 13 of the 20 variants. Two molecular markers (CaM24 and CaM62) detected DNA banding pattern changes in the same four variants, suggesting that these two markers may be located in the same chromosomal segment. Strong association between leaf characteristics (leaf blotching and main vein color) and molecular banding pattern changes with molecular marker CaM42 were observed in six variants, indicating that CaM42 may be associated with gene loci controlling leaf blotching and leaf main vein color in caladium.

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Somaclonal Variation of Caladium Bicolor (Ait.) Vent. 'Jao Ying' After in Vitro Culture Propagation

Cited by 8 publications

References 0 publications

Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Effects of Different Nutrient Solutions on the Acclimatisation of in vitro Caladium Plantlets using a Simplified Hydroponic System

Morphological, cytological and molecular marker analyses of ‘Tapestry’ caladium variants reveal diverse genetic changes and enable association of leaf coloration pattern loci with molecular markers

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