Somatic and sexual hybrids of Petunia hybrida and Petunia parodii

The Genetics of Petunia

“…After the first attempts by Potrykus (1972) using petal or ovule cells, the techniques of protoplast culture, callus proliferation, and plant regeneration were rapidly refined in Germany (Durand et al 1973;Binding 1974a) and Great Britain (Frearson et al 1973). Complementary methods were developed for the whole genus Power et al 1976b;Sink and Power 1977). A complete review of potentialities of protoplast cultures in Petunia as compared to those in tobacco was made by Cocking (1975).…”

“…However, the P. parodii x P. inflata cross is successful only when P. parodii is used as a seed parent. Somatic and sexual hybrids, compared with respect to flower color segregation, do not show significant differences (Power et al 1978). More recently, Power et al (1980) reported success in somatic hybridization between P. parodii and P. parviflora, which could not be sexually crossed.…”

“…PLANT BREEDING REVIEWS hybrida and P. parodii colonies, combined with their differential sensitivity to actinomycin D, allowed Power et al (1976b) to create somatic hybrids of P. hybrida + P. parodii. In the same way, somatic hybrids of P. parodii + P. inflata were obtained by Power et al (1979) by means of an albino mutation carried by P. inflata.…”

Effect of Different Cellulase and Pectinase Enzyme Treatments on Protoplast Isolation and Viability in Lilium ledebeourii Bioss.

“…Leaf mesophyll cells of a wide range of plants have been used with success as a protoplast source (Saker et al, 1999). Protoplasts have been isolated from various genotypes of Petunia × hybrida (Izhar and Power, 1977;Vasil and Vasil, 1974), P. parodii, (Hayward and Power, 1975), P. inflata, P. violocea and P. axillaris (Dulieu et al, 1983;Power et al, 1976). reported that protoplasts of the apical region including leaf primordia proved to be superior to leaves for protoplasts isolation.…”

Incongruity of interspecific and intergeneric crosses involving Nicotiana and Petunia species that exhibit potential for somatic hybridization

“…Likewise, intra-and interspecific somatic hybridization has been reported from three independent research groups utilizing Nicotiana species (CARLSON et al ., 1972, GLEBA et al, 1975, MELCHERS & LABIB, 1974 and one involving Petunia (POWER et al, 1976) ; all four systems combined protoplasts of two species that are sexually congrous . The ultimate objective of somatic hybridization is to combine species that exhibit incongruity at the interspecific or intergeneric level in order to expand the diversity of germ plasm available for crop improvement purposes .…”