Somatic cell hybrids derived from terminally differentiated rhesus cells and established mouse cell lines

Hu, Funan; Pasztor, Linda M.; Teramura, Dinah J.

doi:10.1016/0047-6374(77)90032-x

Cited by 10 publications

(2 citation statements)

References 15 publications

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“…Most studies have involved dermal melanocytes and their melanosomes, which can be highly melanogenic as a result of a variety of conditions 7 –12 . The choroidal melanocyte has been shown to be a postmitotic, terminally differentiated cell that normally ceases any substantial melanogenic activity 13 –16 . The choroidal melanocyte has been examined with regard to development and aging only in the rhesus macaque monkey and most recently just the development was studied in the rat 17 –21 …”

Section: Discussionmentioning

confidence: 99%

The influence of aging and low zinc nutrition on the choroid in the pig: II. The melanosome

Samuelson

Lewis

Mackay

et al. 1999

Veterinary Ophthalmology

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The effects of low zinc nutrition and aging on central choroidal melanosomes were examined in the pig. Melanosomes of central choroidal melanocytes were morphologically and morphometrically examined in three populations of pigs (young, pregnant and aged), that were maintained on either control (C) or low zinc (LZ) diets. In C groups, the typical melanosomes decreased in size with age, although a subpopulation of larger melanosomes occurred in the oldest group. In contrast, the melanosomes of the animals on LZ diets increased in size significantly in the adult groups. The melanosomes in the pregnant and aged groups were 65% and 30-40% greater than those of the age-matched C groups. Extremely large melanosomes were frequently encountered in adult LZ choroidal melanocytes. Melanogenesis of these large bodies included the formation of one or more outer shells. Fusion of adjacent large melanosomes was also observed. Melanolysosomal-like bodies were observed, particularly among the pigmented cells in the large blood vessel region of C and LZ adults. Melanin dynamics, i.e. its production and breakdown, occurred within choroidal melanocytes throughout much of the lifespan of the pig. This dynamic was greatly influenced by low zinc nutrition, resulting in unusual and aberrant melanin activity.

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Section: Discussionmentioning

confidence: 99%

The influence of aging and low zinc nutrition on the choroid in the pig: II. The melanosome

Samuelson

Lewis

Mackay

et al. 1999

Veterinary Ophthalmology

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“…Normal choroidal melanocytes were prepared from the eyes of young adult rhesus and cynomolgus macaques {Macaca mulatta and Macaca fascicularis). The isolation and culture methods have been reported elsewhere (Hu, Pasztor & Teramura, 1977). Cells were grown as short-term cultures, subcultured one to three times and used for experiments within a few weeks.…”

Section: Cell Linesmentioning

confidence: 99%

Effects of dicarboxylic acids on normal and malignant melanocytes in culture

Mah

Teramura

1986

Br J Dermatol

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We have shown that dicarboxylic acids (C9 and C12), known competitive inhibitors of tyrosinase, are selectively cytotoxic to malignant melanogenic melanocytes but not to normal pigmented cells or to amelanotic or non-melanogenic melanoma cells. The main target of this toxicity appears to be the mitochondria, which become markedly swollen and vacuolated. The mechanism of their action has been thought to be due to interference with oxidoreductases in the mitochondria. However, our results suggest that this cytotoxicity most probably does not result simply from inhibition of mitochondrial enzymes, but is closely related to the melanin biosynthesis pathway.

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Persistence of abnormal melanocytes in immunosuppressed chickens of the autoimmune “DAM” line

1984

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The delayed amelanotic (DAM) line of chickens (Gallus gallus) is characterized by the postnatal elimination of melanocytes from regenerating feathers and from the choroid. The process of elimination is accompanied by a massive infiltration of mononuclear leukocytes (MNL) into the supporting connective tissues. When surgically bursectomized at day of hatching, chickens from this lineage develop significantly less amelanosis of the feathers. We report here a histological analysis of regenerating feathers and choroids from bursectomized birds that maintained their plumage pigmentation. In the feathers we observed the presence of morphologically abnormal melanocytes in the absence of MNL infiltration. Choroids also contained abnormal melanocytes without MNL infiltration; however, we observed a few cases of amelanotic choroids with a few MNL. These findings indicate that melanocytes of pigmented birds are morphologically abnormal even in the absence of a bursa and in the absence of leukocytic infiltrates into regenerating feathers and possibly into the choroids. We conclude from these findings that the amelanosis in unbursectomized DAM birds is due to the response of the immune system to an abnormality in the melanocytes which, by itself, does not lead to depigmentation.

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Somatic cell hybrids derived from terminally differentiated rhesus cells and established mouse cell lines

Cited by 10 publications

References 15 publications

The influence of aging and low zinc nutrition on the choroid in the pig: II. The melanosome

The influence of aging and low zinc nutrition on the choroid in the pig: II. The melanosome

Effects of dicarboxylic acids on normal and malignant melanocytes in culture

Persistence of abnormal melanocytes in immunosuppressed chickens of the autoimmune “DAM” line

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