Somatic changes in B-lymphoproliferative disorders (B-LPD) detected by DNA-fingerprinting

Jong, Daphne de; Voetdijk, B M; Kluin-Nelemans, Hanneke C.; Ommen, G J van; Kluin, Philippus

doi:10.1038/bjc.1988.306

Cited by 23 publications

(8 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since D H J H joints can undergo low levels of somatic hypermutation, and since there are usually no mutations at the bcl-2/J H breakpoints (presumably because they are too far downstream of the bcl-2 promoter), it was speculated that the cells had acquired somatic mutations in their D H J H joints in the GC prior to the translocation event. The idea that bcl-2 translocations may occasionally take place in mature B cells in the GC is also supported by an analysis of rare Ig-de®cient follicular lymphomas, many of which carry only one rearranged IgH allele, the one with a bcl-2/IgH translocation (de Jong et al, 1989). Follicular lymphoma is a malignancy of GC B cells, which presumably express a functional BCR when they are antigen-activated and driven into a GC reaction.…”

Section: Rag-mediated Translocation Events In the Gc?mentioning

confidence: 77%

“…Thus, it appears that even B cells that acquire a chromosomal translocation still depend on BCR expression for survival. Although there are some examples of B cell non Hodgkin lymphomas where translocation or mutation events resulted in loss of Ig expression (de Jong et al, 1989), these cases may have acquired some additional transforming events which overcame the dependence on a functional BCR. A notable exception to this rule is classical Hodgkin's lymphoma, where the tumor cells appear to derive from a population of preapoptotic GC B cells that often acquired destructive mutations preventing expression of a BCR (Kanzler et al, 1996;KuÈ ppers and Rajewsky, 1998).…”

Section: Predominant Targeting Of Translocations To Non-productively mentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms of chromosomal translocations in B cell lymphomas

2001

View full text Add to dashboard Cite

Reciprocal chromosomal translocations involving the immunoglobulin (Ig) loci are a hallmark of most mature B cell lymphomas and usually result in dysregulated expression of oncogenes brought under the control of the Ig enhancers. Although the precise mechanisms involved in the development of these translocations remains essentially unknown, a clear relationship has been established with the mechanisms that lead to Ig gene remodeling, including V(D)J recombination, isotype switching and somatic hypermutation. The common denominator of these three processes in the formation of Ig-associated translocations is probably represented by the fact that each of these processes intrinsically generates double-strand DNA breaks. Since isotype switching and somatic hypermutation occur in germinal center (GC) B cells, the origin of a large number of B cell lymphomas from GC B cells is likely closely related to aberrant hypermutation and isotype switching activity in these B cells. Oncogene (2001) 20, 5580 ± 5594.

show abstract

Section: Rag-mediated Translocation Events In the Gc?mentioning

confidence: 77%

Section: Predominant Targeting Of Translocations To Non-productively mentioning

confidence: 99%

Mechanisms of chromosomal translocations in B cell lymphomas

2001

View full text Add to dashboard Cite

show abstract

“…DNA fingerprinting using the highly polymorphic variable number of tandem repeats (VNTRs) (9) also has been applied to the detection of polymorphisms during malignant transformation (10,11) and to studies of clonality oftumors, both primary and metastatic (12,13). The polymerase chain reaction (PCR) (14,15) has been extensively utilized for the detection and characterization of mutations associated with tumor development (16).…”

mentioning

confidence: 99%

Isolation and characterization of allelic losses and gains in colorectal tumors by arbitrarily primed polymerase chain reaction.

Peinado

Malkhosyan

Velázquez

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

224

125

View full text Add to dashboard Cite

show abstract

“…This approach (the original "DNA fingerprinting") was applied to the detection of somatic changes in tumors of diverse type and origin. Specific genomic alterations have been described in human rectal carcinomas (Thein et al, 1987), B-lymphoproliferative disorders (de Jong et al, 1988), Burkitt lymphoma and Wilms tumor (Hayward et al, 1990), ovarian cancer (Boltz et al, 1990), and DMBA-induced mouse liver tumors (Ledwith et al, 1990). This classical DNA fingerprinting is a sensitive assay for genomic rearrangements, but depends on the availability of large amounts of DNA and time-consuming Southern blot techniques.…”

Section: Introductionmentioning

confidence: 97%

Application of inter–simple sequence repeat PCR to mouse models: Assessment of genetic alterations in carcinogenesis

Benavides

Zamisch

Flores

et al. 2002

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

Genomic instability is believed to play a significant role in cancer development by facilitating tumor progression and tumor heterogeneity. Inter-simple sequence repeat (inter-SSR) PCR has been proved to be a fast and reproducible technique for quantitation of genomic instability (amplifications, deletions, translocations, and insertions) in human sporadic tumors. However, the use of inter-SSR PCR in animal models of cancer has never been described. This new technique has been adapted in our laboratory for the analysis of spontaneous and induced mouse tumors. We established the best PCR conditions for each microsatellite-anchored primer and critically evaluated the reproducibility of the band patterns. We also studied the variation of the fingerprints between and within various inbred mouse strains, including wild-derived lines. Tumor-specific alterations were detected as gains, losses, or intensity changes in bands when compared with matched normal DNA. We quantitated the extent of alterations by dividing the number of altered bands in the tumor by the total number of bands in normal DNA (instability index). By means of inter-SSR PCR, we successfully analyzed genomic alterations in various mouse tumors, including spontaneous thymic lymphomas developed in Msh2 knockout mice as well as chemically induced squamous cell carcinomas and thymic lymphomas. Instability index values ranged between 0 and 9%, the highest levels observed in N-methyl-N-nitrosourea-induced thymic lymphomas generated in Trp53 (p53) nullizygote (-/-) mice. We report here, for the first time, the use of inter-SSR PCR to detect somatic mutations in mouse tumoral DNA, including laser-capture microdissected, methanol-fixed tissues. These PCR-based fingerprints provide a novel approach to assessing the number and onset of mutational events in mouse tumors and will help to understand better the mechanisms of carcinogenesis in mouse models.

show abstract

Somatic changes in B-lymphoproliferative disorders (B-LPD) detected by DNA-fingerprinting

Cited by 23 publications

References 7 publications

Mechanisms of chromosomal translocations in B cell lymphomas

Mechanisms of chromosomal translocations in B cell lymphomas

Isolation and characterization of allelic losses and gains in colorectal tumors by arbitrarily primed polymerase chain reaction.

Application of inter–simple sequence repeat PCR to mouse models: Assessment of genetic alterations in carcinogenesis

Contact Info

Product

Resources

About