Somatic chromosome differentiation in Cucumis melo L. and C. metuliferus E.Mey. ex Naudin

Hoshi, Yoshikazu; Kido, Michiko; Yagi, Kouhei; Tagashira, Norikazu; Morikawa, Atsumi; Nagano, Katsuya

doi:10.3199/iscb.8.7

Cited by 8 publications

(8 citation statements)

References 24 publications

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“…In particular, 5S rDNA and 2 minor 45S rDNA signals were found on chromosome 12. The number of 5S and 45S rDNAs was reported in earlier studies [Hoshi et al, 2013]. In this report, 2 major 5S rDNA and 2 major 45S rDNA signals were localized on 2 shortest and 2 middle-sized chromosomes, respectively.…”

Section: Discussionsupporting

confidence: 53%

“…Root-tips from 1-2 cm germinated seeds were collected, pretreated with 0.05% colchicine solution for 2 h at 4 ° C, fixed in 3: 1 ethanol:acetic acid for 1 h on ice, and washed in distilled water for 1 h. Root tip microscope slides were prepared according to previously described protocols [Tagashira et al, 2009;Hoshi et al, 2013].…”

Section: Preparation Of Chromosomesmentioning

confidence: 99%

“…Interestingly, 2 previous studies noted thick heterochromatic bands at the end of chromosomes [Ramachandran and Narayan, 1990;Hoshi et al, 2013], indicating the potential importance of highly repeated heterochromatic DNA for karyotyping in C. metuliferus . However, these reports differed with regard to the methodology used and the structural details revealed.…”

mentioning

confidence: 99%

“…In the case of C. metuliferus , 2 major 45S rDNA and 2 major 5S rDNA signals were detected using a combination of FISH with basespecific fluorescence [Hoshi et al, 2013]. However, exact mapping of the rDNA loci was not performed and a detailed karyogram was not created.…”

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confidence: 99%

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Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of Cucumis metuliferus Using Fluorescence in situ Hybridization

Yagi¹,

Siedlecka²,

Pawełkowicz³

et al. 2014

Cytogenet Genome Res

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Cucumis metuliferus (2n = 24) is a cultivated species of the Cucumis genus which is a potential genetic resource for Cucumis crops. Although some cytogenetic research has been reported, there is no study of karyotyping in this species. Here, we used 4′,6-diamidino-2-phenylindole and chromomycin A3 staining to identify 12 pairs of chromosomes in early-metaphase cells. Fluorescence in situ hybridization revealed the chromosomal distribution patterns of the 5S and 45S ribosomal DNA (rDNA) genes, telomeres, and 3 different satellite repeats. The 2 major signals of the 45S rDNA were located on the satellite of chromosome 11, and the 2 signals of the 5S rDNA and 2 minor signals of the 45S rDNA were located on chromosome 12. The telomere probes hybridized to the ends of all chromosomes. The 3 satellite DNAs were localized at the ends of chromosomes 1, 2, 4-10, and at the end of the short arm of chromosome 3. In summary, we reported the identification of all chromosomes of C. metuliferus. We also depicted the location of 5S and 45S rDNA, the telomere motif sequence, CmetSat1, CmetSatT2, and CmetmSat1 in an ideogram.

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Section: Discussionsupporting

confidence: 53%

Section: Preparation Of Chromosomesmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of Cucumis metuliferus Using Fluorescence in situ Hybridization

Yagi¹,

Siedlecka²,

Pawełkowicz³

et al. 2014

Cytogenet Genome Res

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“…In Brazil, there are approximately 29 genera, comprising 155 species (Gomes-Klein, 2015), where the northeast region alone accounts for 22 genera and 52 species (Gomes-Klein, 2006). Genus Cucumis comprises 32 species (Hoshi et al, 2013), being one of the most important genera in economic terms. It includes many cultivated vegetables such as cucumber, gherkin, and ornamental plants such as gourd and pumpkin.…”

Section: Introductionmentioning

confidence: 99%

Karyotypic characterization of melon accessions

Santos-Sanchês¹,

Souza²,

Melo³

et al. 2019

Científica

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Cucumis melo (melon) is a species from the Iberian Peninsula and is included in the family Cucurbitaceae. Despite the knowledge about the physical structure of melon chromosomes, little is known about the intraspecific karyotype diversity of the species. To study karyotype diversity in eight melon accessions, the following methods were used: Giemsa 3% staining; application of CMA3/DAPI fluorochromes; and location of 45S and 5S rDNA sequences by fluorescence in situ hybridization. Conventional staining analysis revealed stability in the chromosome number, with accessions presenting 2n = 24. There were significant variations in the mean chromosome size between accessions, ranging from 0.98 μm to 1.46 μm for accessions A26 and A18, respectively. Scott-Knott clustering distributed the accessions into two groups. GC-rich heterochromatic blocks (CMA3 + /DAPI-) were observed in pericentromeric regions of all chromosomes in the complement. CMA3 + /DAPIblocks were also located in terminal regions, being specific to satellite regions. Hybridization sites of 45S rDNA probes revealed the presence of a chromosome pair with this locus. In addition, 5S rDNA sites revealed a labeled chromosome pair. No quantitative variation was observed in rDNA sites between the accessions analyzed, indicating these markers as ideal for the verification of karyotypic stability in C. melo.

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