Somatic chromosome mutations and morphological abnormalities in sperms of boars

Rubeš, Jiřı́; Hořínová, Z.; Gustavsson, I.; Borkovec, L.; Urbanová, J.

doi:10.1111/j.1601-5223.1991.tb03548.x

Cited by 10 publications

(2 citation statements)

References 14 publications

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“…Sperm morphology has, moreover, been used as an indicator of biological (Kasa & Thwaits, 1992) and toxicological (Wyrobek et al ., 1983) stress. Sperm morphological examination might be considered to be the most suitable parameter for the evaluation of genotoxic agents affecting sperm quality since other indices of routine semen evaluation are to a large extent influenced by secondary factors (Rubes et al ., 1991). The study by Lee et al .…”

Section: Introductionmentioning

confidence: 99%

Standardization of sampling and staining methods for the morphometric evaluation of sperm heads in the Cynomolgus monkey (Macaca fascicularis) using computer‐assisted image analysis

et al. 1998

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Automated sperm morphology analysis (ASMA) technology has improved the assessment of sperm morphology, but the results depend on the use of adequate and standardized procedures. In this study the Sperm-Class AnalyzerÒ (SCA) ASMA system was used to assess sperm head morphometry in the Cynomolgus monkey and to evaluate the in¯uence of sample size, intraslide variation, and the use of three staining techniques on the accuracy of image processing and sperm head morphometry. Haematoxylin is the staining technique of choice for Cynomolgus spermatozoa, as optimum contrast of sperm heads with the surrounding background allows ef®cient segmentation, i.e. sperm head boundary detection, making the image analysis process more accurate. The analysis of 100 spermatozoa is recommended since a larger sample size did not result in more accurate sperm head morphometry. There were no differences in either the percentage of correctly binarized sperm heads or sperm head dimensions among samples obtained from different zones of the slides, although differences in stain intensity (grey level) were detected. The measurements made on Haematoxylin, Diff-Quik and Hemacolor-stained slides yielded different values for all of the sperm head parameters under consideration. This result demonstrates that the procedures of ®xation and staining signi®cantly affect the dimensions of sperm heads.

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Section: Introductionmentioning

confidence: 99%

Standardization of sampling and staining methods for the morphometric evaluation of sperm heads in the Cynomolgus monkey (Macaca fascicularis) using computer‐assisted image analysis

et al. 1998

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“…В целях прогнозирования репродуктивного успеха при применении биотехнологических методов воспроизводства в животноводстве успешно используется такой биологический показатель, как частота встречаемости различных характеристик геномной нестабильности соматических клеток, ассоциированный с изменчивостью частот появления аномальных сперматозоидов [11]. К таким показателям, в частности, относится количество эритроцитов с микроядрами, отражающее потенциальную геномную нестабильность доноров спермы.…”

Section: введение/Introductionunclassified

Analysis of the relationship between the frequency of erythrocytes with micronuclei, semen quality and reproductive indicators in artificial insemination of foxes

Popov,

Glazko,

Glazko

et al. 2024

Agrarnaâ nauka

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Relevance. Reproductive biotechnologies are widely used in various branches of animal husbandry. Their scientifically based application shows high results in obtaining offspring. In fur-bearing cellular animal husbandry, reproductive biotechnology methods also give certain results. At the same time, in order to increase and predict the success in reproduction of fur-bearing animals, it is necessary to control the selection and selection of parental individuals, in particular male sperm donors during artificial insemination.Methods. The studies were performed in February — May 2023. The object of the study were ejaculate samples, peripheral blood smears of 16 silver-black fox males, as well as the corresponding results of artificial insemination (AI). To evaluate ejaculates, the following were studied: sperm concentration — by photometry, their percentage distribution by type of movement — on the ISAS system, sperm morphology — by microscopy. In peripheral blood smears, the frequency of occurrence of erythrocytes with micronuclei was determined using a micronucleus test (MTA). The results of the IO were evaluated by the number and percentage of pregnant and missing females, the number of live and dead puppies born.Results. The results showed that with the frequency of occurrence of erythrocytes with micronuclei above 2%, the sperm content in ejaculates was from 45 to 54.5% with non-accessible movement (type c), and from 22.7 to 44.2% stationary (type d). Also, abnormal sperm forms prevailed in the ejaculates of these males (63–83%), while 66–100% of missing females were noted. At the same time, males with MINTS of less than 1.5% demonstrated good ejaculate quality and high efficiency of artificial insemination.

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Induction of chromosomal aberrations, sister-chromatid exchanges and cell-cycle delay in cow peripheral blood lymphocytes treatment with two N-nitroso compounds in vitro

Šiviková

Dianovský

1993

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Somatic chromosome mutations and morphological abnormalities in sperms of boars

Cited by 10 publications

References 14 publications

Standardization of sampling and staining methods for the morphometric evaluation of sperm heads in the Cynomolgus monkey (Macaca fascicularis) using computer‐assisted image analysis

Standardization of sampling and staining methods for the morphometric evaluation of sperm heads in the Cynomolgus monkey (Macaca fascicularis) using computer‐assisted image analysis

Analysis of the relationship between the frequency of erythrocytes with micronuclei, semen quality and reproductive indicators in artificial insemination of foxes

Induction of chromosomal aberrations, sister-chromatid exchanges and cell-cycle delay in cow peripheral blood lymphocytes treatment with two N-nitroso compounds in vitro

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