Somatic embryo production from immature seeds of carob (<i>Ceratonia siliqua</i>L.): histological evidence

Ksia, E.; Harzallah‐Skhiri, Fethia; Verdeil, Jean‐Luc; Gouta, H.; Alemanno, Laurence; Bouzid, Sadok

doi:10.1080/14620316.2008.11512398

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolated or combined use of 2,4-D with other growth regulators, mainly cytokinins, has been commonly used for the induction of somatic embryogenesis via tissue culture of seeds and zygotic embryos for several species. In most embryogenic models induced in vitro, 2,4-D is required for the initiation of cell programming via somatic embryogenesis (Fehér et al 2003;Raghavan 2004;Fehér 2005;Ksia et al 2008).…”

Section: Resultsmentioning

confidence: 99%

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Silva

Pinto

Guerra

et al. 2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The objective of the present work was to induce somatic embryogenesis from zygotic embryos of Passiflora cincinnata Masters. Zygotic embryos formed calli on media with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.5 lM benzyladenine (BA) after 30 days of in vitro culture. A concentration of 18.1 lM 2,4-D resulted in the largest number of somatic embryos. Embryogenic calli were yellowish and friable, forming whitish proembryogenic masses. Morphologically, embryogenic cells were small and had large nuclei and dense cytoplasm, whereas non-embryogenic cells were elongated, with small nuclei and less dense cytoplasm. Calli cultured under white light on basal Murashige and Skoog's medium with activated charcoal produced embryos in all developmental stages. There were differences among the treatments, with some leading to the production of calli with embryos and some only to callus formation. Some abnormalities were associated with somatic embryos, including fused axes, fused cotyledons and polycotyledonary embryos. Production of secondary somatic embryos occurred in the first cycle of primary embryo development. Secondary embryos differentiated from the surface of the protodermal layer of primary embryos with intense cell proliferation, successive mitotic divisions in the initial phase of embryoid development, and a vascular system formed with no connection to the parental tissue. This secondary embryogenic system of P. cincinnata is characterized by intense proliferation and maintenance of embryogenic competence after successive subcultures. This reproducible protocol opens new prospects for massive propagation and is an alternative to the current organogenesis-based transformation protocol.

show abstract

Section: Resultsmentioning

confidence: 99%

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Silva

Pinto

Guerra

et al. 2009

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…For carob, NAA was successfully used for callogenesis from hypocotyls (75%) and cotyledons (57.1%) with 1 mg/l NAA and 2 mg/l BAP [12], apex of young seedlings (66-80%) in the presence of 1 mg/l 8 BAP associated with 0.1 mg/l NAA [15], also with nodal segments of young seedlings in a media containing 1 mg/l BAP in combination with 1 mg/l NAA [19]. However, for other studies on carob, the best results for callus induction were obtained with 2,4-D in the case of roots (28.6%) [12], anthers (100%) [29], immature seeds (67%) [30], portions of hypocotyls (58.3%) and cotyledons (100%) [18] and mature cotyledons of seed (100%) [31]. Among the concentrations of BAP combined with NAA, it was found that concentrations 2.22 and ar results without significant difference, in particular when the concentration of NAA is equivalent to 5 μM.…”

Section: Discussionmentioning

confidence: 99%

“…During the proliferation phase, we did not observe primary somatic embryogenesis. Contrariwise, Custodio et al [29], Ksia et al [30] and Lozzi et al [31] induced primary somatic embryogenesis from the obtained calli.…”

Section: Discussionmentioning

confidence: 99%

“…Culture of different carob explants: from parts of young seedlings (cotyledonary leaves, hypocotyl, roots, meristems, lateral and axillary buds) [12][13][14][15][16][17][18][19][20][21], meristems, lateral and axillary buds of adult female trees [4,13,[22][23][24][25][26][27], ova taken from female flowers [28], anthers of the male inflorescences of seed trees [29], immature seeds [30], mature cotyledons of seeds [31] and fruit tissue [32] is often accompanied by the formation of a callus whose importance varies according to the conditions studied.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Callus Induction from Carob (Ceratonia siliqua L.) Seedlings and Leaves of Mature Tree

Bouzdoudi

Saidi

Ansari

et al. 2017

ARRB

View full text Add to dashboard Cite

Callus induction was successfully carried out from several explants of carob (Ceratonia siliqua L.). Callogenesis from the apex was tested on three different media containing Woody Plant Medium (WPM), Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) macronutrients supplemented with two different hormonal solutions: benzylaminopurine (BAP) at 4.44 µM alone, or 2.22 µM of BAP plus 5 µM of 2-naphthalineacetic acid (NAA). Primary callus formation was obtained on a medium containing 88% WPM macronutrients. Callus formation from other parts of the plant was as follows: Cotyledon embryos extracted from immature seeds (85% success rate on WPM medium, containing 4.44 µM BAP and 5 µM NAA);  Cotyledon leaves taken from 7-day-old seedlings, obtained from in vitro germination of seeds (62% success rate on WPM medium, containing 4.44 µM BAP and 5 µM NAA);

show abstract

“…Plant growth regulators and optimization of their proper dosages are also very important for success in in vitro culture (Kouassi et al, 2017;Bhattacharjee and Islam, 2014;Morshed et al, 2014). The somatic and gametic embryogenesis on major cereal crops report that regeneration capability depends on genotype (Ksia et al, 2008;Islam et al, 2001). Some methods like mutation and haploid breeding, somaclonal variation, genetic engineering etc.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Somatic Embryogenesis by Mature and Immature Seeds in Wheat (Triticum aestivum L.)

Saha¹,

Islam²,

Islam³

et al. 2017

JBLS

View full text Add to dashboard Cite

A suitable plant regeneration system has been established using 3-4 weeks old calli derived from immature and mature seeds of four wheat varieties viz. Pavon 76, Akbar, Barkat, and Kanchan. As plant growth regulators, various auxins (2,4-D, BAP and IAA) either single or in combination were used in MS medium. The variety Pavon 76 showed maximum (72.25%) callus induction and Akbar exhibited the lowest (37.78%) from calli derived from immature seeds. Hormonal effects on callus induction were evaluated and significant results were found in case of genotypes at P <0.01. Out of four genotypes, the highest frequency of plant regeneration was recorded in Pavon 76 (67.00%) and lowest in Kanchan (43.10%) when 1.5 mg/l BAP and 0.5 mg/l IAA was added in the medium. It was observed that Pavon 76 produced highest number of green plants than others. For mature seeds all of the mentioned genotypes showed significant difference with maximum frequency of callusing in Pavon 76 (69.57%) in MS + 2.5 mg/l 2,4-D followed by Kanchan (60.84%), Barkat (52.73%), and Akbar (47.19%). For plant regeneration, Pavon 76 also showed best performance (64.36%) in MS + 2.0 BAP + 1.0 mg/l IAA, using calli derived from mature seeds. The other genotypes Barkat, Kanchan and Akbar exhibited 59.44, 52.71 and 52.32% regeneration in the same medium respectively. Here, the lowest regeneration (40.63%) was found in Akbar. In this case, it was aimed to establish a suitable protocol for in vitro callus induction and regeneration for advance biotechnological research on wheat in Bangladesh.

show abstract

Somatic embryo production from immature seeds of carob (Ceratonia siliquaL.): histological evidence

Cited by 7 publications

References 0 publications

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

Callus Induction from Carob (Ceratonia siliqua L.) Seedlings and Leaves of Mature Tree

Enhancement of Somatic Embryogenesis by Mature and Immature Seeds in Wheat (Triticum aestivum L.)

Contact Info

Product

Resources

About