Somatic Embryogenesis and Its Application for Breeding and Micropropagation in Asparagus (Asparagus officinalis L.).

Role of biotechnology in medicinal plants

“…However, Arumugam and Bhojwani noted that inclusion of BA (2 µM) and gibberellic acid (GA3, 2.8 µM) in the medium stimulated embryo development of Podophyllum hexandrum, although 75% of the embryos germinated on basal MS medium devoid of growth regulator 38 . Similar results were reported on the germination of embryos of Psoralea corylifolia Further, Kunitake and Mii reported that 30-40% of somatic embryos of A. officinalis germinated after being treated with distilled water for a week; they were subsequently transferred to half-strength MS medium supplemented with 1.0 mg/L IAA, 1.0 mg/L GA3 and 1% sucrose 50 . However, the somatic embryos of Typhonium trilobatum have been germinated on MS medium supplemented with 0.01 mg/L NAA and 2% (w/v) sucrose after 2 weeks of culture 45 .…”

Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

“…Therefore, the use of micropropagation methods adapted to the different species would be necessary to propagate the materials. Different micropropagation procedures have been published for different species of the genus Asparagus such as: A. officinalis (Desjardins et al 1987;Kunitake and Mii 1998;Raimondi et al 2001Raimondi et al ), A. maritimus (S ˇtajner et al 2002, A. densiflorus (Benmoussa et al 1996), A. plumosus (Ghosh and Sen 1994a), A. verticillatus (Ghosh and Sen 1996), A. robustus (Nayak and Sen 1998), A. cooperi (Ghosh and Sen 1994b) and A. racemosus (Bopana and Saxena 2008). However, a general protocol has not been reported that is valid for all the species of Asparagus and interspecific hybrids between them.…”

Clonal propagation of Lomandra longifolia by somatic embryogenesis

“…In the Liliaceous monocots, plant regeneration from organogenic or embryogenic callus or suspension cultures was demonstrated for several species, including Agapanthus africanus (Supaibulwatana and Mii, 1997), Allium spp. (Kim and Soh, 1996), Asparagus officinalis (Kunitake and Mii, 1998), Hemerocallis spp. (Krikorian and Kann, 1981), Lilium spp.…”