Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture

Ganesan, M.; Chandrasekar, R.; Kumari, B. D. Ranjitha; Jayabalan, N.

doi:10.1007/s10535-007-0090-0

Cited by 16 publications

(7 citation statements)

References 27 publications

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“…In this study, 30 g sucrose was found to be the best for embryogenic callus induction. These results were similar to those reported by Ganesan et al (2007) in okra and in black iris by Shibli and Ajlouni (2000). For successful regeneration of synthetic seeds from the somatic embryos, good quality embryogenic callus is essential (Ganesan and Jayabalan 2004).…”

Section: Resultssupporting

confidence: 93%

In vitro Development of Cauliflower Synthetic Seeds and Development of Plantlets In vivo

Qamar

Hossain

Nasir

et al. 2014

Plant Tissue Cult. & Biotech.

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Synthetic seeds of cauliflower cv. Chillout were developed by encapsulating mature somatic embryos in neutral gel media. Somatic embryos were obtained by optimizing callus and cell suspension cultures of cauliflower. Friable, yellowish embryogenic calli were obtained on MS supplemented with 2 mg/l 2,4-D and 0.5 mg/l BAP using hypocotyl as explants, while calli were regenerated in media consisting of 5 mg/l BAP, 2 mg/l Kn and 6 mg/l GA3. Somatic embryo-genesis was induced in cell suspension culture where auxins were removed in successive steps triggering conversion of globular cells into the heart, torpedo stage (71%) and finally into cotyledonary/somatic embryos (28%). The mature somatic embryos were encapsulated by mixing mature cell suspension with sodium alginate and calcium chloride mixture (1 : 4). Developed synthetic seeds germinated into complete plantlets when placed in neutral gel media. Germination efficiency of synthetic seeds decreased to about 50 per cent after 12 weeks of storage at 4ºC followed by a rapid decrease to zero per cent after 16 weeks. It was also observed that cauliflower plantlets from synthetic seeds survived successfully when transferred to soil demonstrating that cauliflower synthetic seeds is a promising step towards their in vivo direct use. Plant Tissue Cult. & Biotech. 24(1): 27-36, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19193

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Section: Resultssupporting

confidence: 93%

In vitro Development of Cauliflower Synthetic Seeds and Development of Plantlets In vivo

Qamar

Hossain

Nasir

et al. 2014

Plant Tissue Cult. & Biotech.

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“…These results show that cytokinins (BA and zeatin) have stimulatory roles for plantlet development from embryogenic cell suspension cultures of watershield. A stimulatory role for cytokinin in plantlet development from somatic embryos was also reported for Abelmoschus esculentus (Ganesan et al 2007), and Ophiorrhiza prostrata (Martini et al 2007). …”

Section: Effects Of Activated Charcoal and Zeatin On Plantlet Developmentioning

confidence: 58%

High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)

Choi

et al. 2008

Plant Biotechnol Rep

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An improved protocol for high frequency plant regeneration via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield (Brasenia schreberi) was developed. Zygotic embryos formed pale-yellow globular structures and white friable callus at a frequency of 80% when cultured on halfstrength MS medium supplemented with 0.3 mg l -1 2,4-D. However, the frequency of formation of pale-yellow globular structures and white friable callus decreased slightly with increasing concentrations of 2,4-D up to 3 mg l -1 , where the frequency reached *50% of the control. Cell suspension cultures from zygotic embryoderived white friable callus were established using halfstrength MS medium supplemented with 0.3 mg l -1 2,4-D. Upon plating of cell aggregates on half-strength MS basal medium, approximately 8.3% gave rise to somatic embryos and developed into plantlets. However, the frequency of plantlet development from cell aggregates was sharply increased (by up to 55%) when activated charcoal and zeatin were applied. Regenerated plantlets were successfully transplanted to potting soil and grown to normal plants in a growth chamber. The distinctive feature of this study is the establishment of a high frequency plant regeneration system via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield, which has not been previously reported. The protocol for plant regeneration of watershield through somatic embryogenesis could be useful for the mass propagation and transformation of selected elite lines.

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“…Tissue culture-based direct shoot regeneration from cotyledon and cotyledonary node explants (Mangat and Roy, 1986 ), and regeneration of okra plants from callus tissue derived from cotyledonary axil (Roy and Mangat, 1989 ) are known. Further, Ganesan et al ( 2007 ) have reported plant-regeneration protocol through somatic embryogenesis from suspension culture; while Anisuzzaman et al ( 2008a ) developed a protocol for mass in vitro propagation using meristem culture for disease-free plant production. Anisuzzaman et al ( 2008b ) have optimized a viable protocol for indirect shoot organogenesis of okra, from leaf-disc and hypocotyl via callus phase.…”

Section: Transformation Protocols and Transgenic Development In Okramentioning

confidence: 99%

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

et al. 2017

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Despite the importance of okra, as one of the important vegetable crop, very little attention has been paid to its genetic improvement using advanced biotechnological tools. The exploitation of marker assisted breeding in okra is often limited due to the availability of a few molecular markers, the absence of molecular genetic-map(s), and other molecular tools. Chromosome linkage-groups were not yet constructed for this crop and reports on marker development are very scanty and mostly hovering around cultivar characterization. Besides, very little progress has been observed for transgenic development. However, high throughput biotechnological tools like chromosome engineering, RNA interference (RNAi), marker-assisted recurrent selection (MARS), genome-wide selection (GWS), targeted gene replacement, next generation sequencing (NGS), and nanobiotechnology can provide a rapid way for okra improvement. Further, the etiology of many deadly viral diseases like the yellow vein mosaic virus (YVMV) and okra enation leaf curl virus (OELCV) in okra is broadly indistinct and has been shown to be caused by various begomovirus species. These diseases cause systemic infections and have a very effective mode of transmission; thus, preventing their spread has been very complicated. Biotechnological interventions have the potential to enhance okra production even under different viral-stress conditions. In this background, this review deals with the biotechnological advancements in okra per se along with the begomoviruses infecting okra, and special emphasis has been laid on the exploitation of advanced genomic tools for the development of resistant varieties.

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Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture

Cited by 16 publications

References 27 publications

In vitro Development of Cauliflower Synthetic Seeds and Development of Plantlets In vivo

In vitro Development of Cauliflower Synthetic Seeds and Development of Plantlets In vivo

High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

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