Somatic Embryogenesis and Plant Regeneration From Immature Cotyledons of Prunus Mume 'Nanko'

Tsukamoto, Tatsuya; Gao, Mei; Negoro, Keiichi; Hanada, Hiromi; Tao, Ryutaro; Kawabe, M.; Yonemori, Keizo

doi:10.17660/actahortic.2007.738.92

Cited by 6 publications

(3 citation statements)

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“…Hence, developing an efficient in vitro technique for the propagation of pomegranate is of great importance. During the past decade, tissue culture techniques have been widely used for the propagation of some important tropical and subtropical fruit trees (Agarwal et al 2004;Tsukamoto et al 2007;PerezTornero et al 2010;Skiada et al 2010).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

ValizadehKaji

Ahmad

Tohidfar

2013

Physiol Mol Biol Plants

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Section: Introductionmentioning

confidence: 99%

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

ValizadehKaji

Ahmad

Tohidfar

2013

Physiol Mol Biol Plants

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“…Tissue culture propagation via either organogenesis or embryogenesis has already been demonstrated in a number of woody species (Litz et al 1985;Hutchinson and Zimmerman 1987;Agarwal et al 2004;Chaugule et al 2005;Tsukamoto et al 2007). Although both in vitro organogenesis and somatic embryogenesis are practical methods of propagation, the latter is preferable for efficient propagation, production of synthetic seeds, and genetic modification.…”

Section: Introductionmentioning

confidence: 99%

Comparison of in vitro regeneration pathways in Punica granatum L.

Kanwar

Joseph

Deepika

2009

Plant Cell Tiss Organ Cult

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When cotyledonary explants, excised from in vitro germinated seedlings, of pomegranate (Punica granatum L.) were incubated on solid Murashige and Skoog (1962) medium supplemented with 21 lM naptheleneacetic acid (NAA) and 9 lM 6-benzyladenine (BA), 80% of explants developed callus. A high frequency of shoot organogensis was obtained when explants were incubated on MS medium supplemented with 8 lM BA, 6 lM NAA, and 6 lM giberrellic acid (GA 3 ). However, adding 24 lM silver nitrate (AgNO 3 ) to this medium markedly enhanced shoot regeneration frequency (63%) and mean number of shoots per explant (11.26) and length of shoots (2.22 cm). Highest frequency of in vitro rooting, mean number of roots/shoot (4.32), and mean root length (2.71 cm) were obtained when regenerated shoots were transferred to halfstrength MS medium supplemented with 0.02% activated charcoal. Well-rooted plantlets were acclimatized, and then transferred to soil medium. Moreover, when zygotic embryos of P. granatum, excised from seeds collected at 16 weeks following full bloom, were incubated on MS medium containing 30 g l -1 sucrose, 15% coconut water, 21 lM NAA, and 9 lM BA, they developed the highest frequency of embryogenic callus, clumps with globular embryos, and mean number of both globular and heartshaped embryos per callus clump. Subjecting zygotic embryo explants to six-week dark incubation period was essential for embryogenic callus induction, and these were subsequently transferred to 16 h photoperiod for further growth and development of somatic embryos. Germination of somatic embryos was observed when these were transferred to MS medium was supplemented with 60 g l -1 sucrose.

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“…During the past decade, tissue culture techniques have been widely used for the propagation of some important tropical and subtropical fruit trees [12][13][14][15]. It has enabled mass propagation of superior genotypes of both wild and cultivated varieties.…”

Section: Introductionmentioning

confidence: 99%

Observation of GFP Expression in Pomegranate (<i>Punica granatum</i> L.) Via Pollen-Mediated Transformation

Yang¹

2016

PLANT

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Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.

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Somatic Embryogenesis and Plant Regeneration From Immature Cotyledons of Prunus Mume 'Nanko'

Cited by 6 publications

References 0 publications

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

Comparison of in vitro regeneration pathways in Punica granatum L.

Observation of GFP Expression in Pomegranate (<i>Punica granatum</i> L.) Via Pollen-Mediated Transformation

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Somatic Embryogenesis and Plant Regeneration From Immature Cotyledons of Prunus Mume 'Nanko'

Cited by 6 publications

References 0 publications

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

Comparison of in vitro regeneration pathways in Punica granatum L.

Observation of GFP Expression in Pomegranate (&lt;i&gt;Punica granatum&lt;/i&gt; L.) Via Pollen-Mediated Transformation

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Product

Resources

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Observation of GFP Expression in Pomegranate (<i>Punica granatum</i> L.) Via Pollen-Mediated Transformation