Somatic embryogenesis and plant regeneration in Floribunda rose (Rosa hybrida L.) cvs. Trumpeter and Glad Tidings

Marchant, R.; Davey, M. R.; Lucas, John A.; Power, J. B.

doi:10.1016/s0168-9452(96)04479-2

Cited by 74 publications

(36 citation statements)

References 13 publications

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“…In other species, the addition of amino acids to the culture medium has been shown to improve embryogenesis. Glutamine and casein hydrolysate have been used to promote embryogenesis in peaches and nectarines (Raj Bhansali et al 1990) and eucalyptus (Pinto et al 2002), and l-proline has been reported to enhance somatic embryogenesis in the early stages of floribunda rose culture (Marchant et al 1996) and miscanthus (Holme et al 1997). …”

Section: Amino Acidsmentioning

confidence: 99%

Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink

Cheong

Pooler

2004

Plant Cell Reports

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Factors affecting somatic embryogenesis from root explants of Prunus incisa Thunb. cv. February Pink were investigated. Using a medium containing Murashige and Skoog salts and vitamins supplemented with 10 microM 2,4-dichlorophenoxyacetic (2,4-D), we evaluated the effects of light, growth regulators, amino acids, carbohydrate source, and root induction medium. Explants cultured under light or dark conditions both resulted in the formation of embryos. Embryogenesis was inhibited by the addition of 6-benzyladenine, thidiazuron, or gibberellic acid to the medium. Amino acids were not effective in promoting embryogenesis, with high levels of amino acids actually inhibiting it. Sucrose and glucose effectively induced embryogenesis, while sorbitol and mannitol completely inhibited it. Sucrose and glucose also promoted secondary embryogenesis. Embryos that formed in medium containing 4% or 5% sucrose were abnormally shaped and did not fully develop, while those that formed in medium with sucrose concentrations of 2% or 3% were much more vigorous. Root explants that were induced on medium containing 1.0 micro M indole-3-butyric acid (IBA) produced more somatic embryos than explants induced on medium without IBA. Approximately 50% of the roots induced on medium containing 1.0 microM IBA produced somatic embryos on medium containing 10 microM 2,4-D and 3% sucrose.

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Section: Amino Acidsmentioning

confidence: 99%

Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink

Cheong

Pooler

2004

Plant Cell Reports

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“…Different concentrations of the two antibiotics, cefotaxime (200 mg L -1 ) and hygromycin (5,7,10,15,25,50 and 100 mg L -1 ) were tested in media to study the effects on survival and maintenance of organogenesis of callus induced from leaf tissue. Portions of organogenic callus (approximately 3-5 mm in diameter) were transferred from the embryo proliferation (EP) medium to the surface of the modified embryo maturation (EM) medium (Marchant et al 1996), which consisted of SH basal salts and vitamins with 2,4-Dichlorophenoxy acetic acid (2,4-D; 1 mg L -1 ), ABA (abscisic acid, 1 mg L -1 ), GA 3 (3 mg L -1 ), sucrose (30 g L -1 ) and agarose at pH 5.4. After 4 weeks, the number (percentage) of calluses retaining organogenesis was noted.…”

Section: Agrobacterium-mediated Transformation Of China Rosementioning

confidence: 99%

DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M. truncatula and China Rose (Rosa chinensis Jacq.)

Chen

Liu

et al. 2010

Plant Growth Regul

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We isolated a DREB orthologue, MtDREB1C, from Medicago truncatula. Its deduced protein contains an AP2 domain of 57 amino acids. Yeast one-hybrid assay revealed that MtDREB1C specifically bound to the dehydration-responsive element (DRE) and activated the expression of HIS3 and LacZ reporter genes. In a transcriptional activation assay, coexpression of the MtDREB1C cDNA resulted in much higher (21.2 times) transactivation of the LacZ reporter gene than experiments performed without MtDREB1C. Transformation of Medicago revealed that overexpression of MtDREB1C suppressed shoot growth, and enhanced the freezing tolerance of M. truncatula. The MtDREB1C gene was transformed into China Rose (Rosa chinensis Jacq.) driven by Arabidopsis rd29A promoter. Southern-blot analysis showed that the target gene was integrated into the genome of a surviving transgenic rose plant. Northern-blot analysis illustrated that robust expression of MtDREB1C was only activated under stress conditions, and the expressed MtDREB1C mRNA reached maximum accumulation 10 h following freezing treatment. The performance of the transgenic line under freezing stress was superior to untransformed controls. This transgenic plant continued to grow, flowered under unstressed conditions, and was phenotypically normal. These facts indicate that the MtDREB1C gene, isolated from Medicago truncatula and driven by the Arabidopsis rd29A promoter, enhanced freezing tolerance in transgenic China Rose significantly without any obvious morphological or developmental abnormality.

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“…Somatic embryos of the two floribundas, Trumpeter and Glad Tidings, converted to plants at a 9-17% frequency (Marchant et al 1996). The hybrid tea rose Royalty showed a 12% conversion rate, while another hybrid tea, Arizona, was reported to have a 38% maturation and a 93% conversion rate (Noriega and Sondahl 1991;Murali et al 1996).…”

Section: Introductionmentioning

confidence: 98%

“…For example, the maturation of somatic embryos was found by Murali et al (1996) to be affected by abscisic acid, while Marchant et al (1996) used abscisic acid combined with BAP. Phloroglucinol was required for the conversion of Arizona embryos (Murali et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Phloroglucinol was required for the conversion of Arizona embryos (Murali et al 1996). Either BAP combined with indole-3-butyric acid and a 4C cold treatment (Marchant et al 1996) or methyl laurate and BAP (Sarasan et al 2001) were shown to stimulate conversion. Regeneration of the rootstock Moneyway was inhibited by BAP (van der Salm et al 1996).…”

Section: Introductionmentioning

confidence: 99%

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Dispersal and size fractionation of embryogenic callus increases the frequency of embryo maturation and conversion in hybrid tea roses

et al. 2004

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Plant regeneration from embryogenic cells of two Rosa hybrida cultivars, Kardinal and Classy, was increased by dispersing embryogenic callus in liquid medium for 3 h followed by size-fractionation to isolate proembryogenic masses that were smaller than 530 microm. Dispersed callus of three cultivars, Kardinal, Classy, and Tineke, produced 61-135 cotyledonary-stage embryos/100 mg fresh weight (FW) as compared to intact callus that had not been dispersed, which produced only zero to three cotyledonary-stage embryos/100 mg FW. Over 500 cotyledonary-stage embryos/100 mg FW callus developed from proembryogenic masses of Kardinal, Classy, and Tineke following 2 months of culture on solidified Murashige and Skoog's basal salts medium supplemented with 0.25% activated charcoal. Cotyledonary-stage embryos of Classy that developed from both dispersed callus and fractionated cells of various sizes showed a significantly higher conversion frequency to plants (28%) than cotyledonary-stage embryos isolated from intact callus (9%). The highest conversion frequencies for Kardinal (50-58%) occurred from cotyledonary-stage embryos that developed from dispersed callus and from the fraction of cells smaller than 850 microm.

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Somatic embryogenesis and plant regeneration in Floribunda rose (Rosa hybrida L.) cvs. Trumpeter and Glad Tidings

Cited by 74 publications

References 13 publications

Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink

Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink

DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M. truncatula and China Rose (Rosa chinensis Jacq.)

Dispersal and size fractionation of embryogenic callus increases the frequency of embryo maturation and conversion in hybrid tea roses

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