Somatic embryogenesis and plant regeneration in açaí palm (Euterpe oleracea)

Scherwinski‐Pereira, Jonny Everson; Guedes, Rodrigo da Silva; Silva, Ricardo Alexandre da; Fermino, Paulo César Poeta; Luis, Zanderluce Gomes; Freitas, Elínea de Oliveira

doi:10.1007/s11240-012-0115-z

Cited by 40 publications

(29 citation statements)

References 26 publications

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“…A similar aspect occurred during the ontogenetic study of somatic embryogenesis of Euterpe oleracea from zygotic embryos, and the somatic embryo induction at diff erent stages of maturation was observed, as well as the formation of secondary somatic embryogenesis (Scherwinski-Pereira et al, 2012). Some plant cells may acquire competence at diff erent stages of tissue exposure to the culture medium plus growth regulators.…”

Section: Discussionsupporting

confidence: 53%

AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus

et al. 2019

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The objective of this study was to evaluate the effect of auxin pulse intervals on the induction of somatic embryos of Eucalyptus grandis x E. urophylla and to describe the embryogenic behavior of callus under the effect of auxinic stress. Cotyledons were inoculated in culture medium containing 207.07 µM picloram, a treatment considered as auxin pulse. Explants that were in the auxin pulse treatment were transferred to semisolid or liquid medium containing 20.71 µM picloram after one, two, four or eight days of auxin pulse. In a second experiment, explants that were on auxin pulse treatment were transferred to semi-solid medium containing 20.71 µM picloram after one, two or three days of auxin pulse. Auxiliary picloram pulse treatments (207.02 µM) can be used as an initial source of stress for the acquisition of embryogenic competence. The oxidation of cotyledonary explants may be considered as an indication of the formation of embryogenic calli. The presence of pectins in peripheral regions of somatic pro-embryos can be considered as a marker of somatic embryogenesis in cotyledonary explants of Eucalyptus grandis x E. urophylla.

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Section: Discussionsupporting

confidence: 53%

AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus

et al. 2019

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“…The initial cell divisions occurred in the epidermal and subepidermal cells of the explant. This regeneration pathway is common in several monocots including river lily (Slabbert et al 1995) and açaí palm (Scherwinski-Pereira et al 2012). Further, the formation of embryogenic cells with prominent nuclei, small vacuoles, and dense cytoplasm was common for several plant species (Paul et al 2011).…”

Section: Discussionmentioning

confidence: 95%

Direct somatic embryogenesis and plant regeneration from leaf sheath explants of mango ginger (Curcuma amada Roxb.)

Raju¹,

Aslam²,

Kathiravan

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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An efficient and simple procedure was systematically developed for inducing direct somatic embryogenesis and plantlet regeneration from leaf sheath explants of Curcuma amada Roxb. A two-step culture system was used to induce somatic embryogenesis. The optimized procedure resulted in direct somatic embryogenesis from 93.3% explants after 3-wk culture. Leaf sheath explants were incubated for 2 wk on medium containing 2.24 μM 2,4-dichlorophenoxyacetic acid and 1.11 μM 6-benzyladenine to initiate direct somatic embryogenesis. Thereafter, these explants were transferred to a medium containing 9.10 μM thidiazuron and 1.33 μM α-naphthaleneacetic acid. Elongated somatic embryos obtained from these cultures germinated readily, and the optimal frequency of plantlet development (86.7%) was achieved when embryos were cultured in darkness on 1/2 strength Murashige and Skoog medium containing 1.44 μM gibberellic acid. Histological and scanning electron microscopic studies showed that the initial cell divisions that led to embryo formation occurred in epidermal and subepidermal cells, followed by the development of globular and elongated structures that appeared to be somatic embryos. The presence of a clear protoderm in the globular structures and procambial strands in the elongated structures confirmed that these structures were true somatic embryos. Plantlets derived from somatic embryos were acclimatized successfully to ex vitro conditions at a survival rate of 87.43% and developed with normal phenotypes.

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“…20.6a-c). This regeneration pathway is common in several monocots including river lily (Slabbert et al 1995) and açaí palm (Scherwinski-Pereira et al 2012). Further, the formation of embryogenic cells with prominent nuclei, small vacuoles, and dense cytoplasm was common for several plant species (Paul et al 2011).…”

Section: Histological Analysismentioning

confidence: 95%

Direct and Indirect Somatic Embryogenesis in Mango Ginger (Curcuma amada Roxb.)

Shajahan

Raju

Thilip

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Somatic embryogenesis is a developmental restructuring of somatic cells in the embryogenic pathway and forms a basis for the concept of totipotency in plant cells. Understanding the process of transition from vegetative to embryogenic cells and factors involved in the somatic embryogenesis and subsequent plant development represents a challenge for plant tissue culture studies in any selected species. Although several reviews have been published dealing somatic embryogenesis in several plant species, there is no recent information on somatic embryogenesis in mango ginger (Curcuma amada). Two different types of somatic embryogenetic pathways are available in mango ginger. They are indirect somatic embryogenesis (IDSE) and direct somatic embryogenesis (DSE). Indirect somatic embryogenesis occurs in friable embryogenic callus from leaf sheath explants that undergo an extreme proliferation before the development of somatic embryos, whereas in the direct somatic embryogenesis, two-step system of culture was followed. Initially, leaf sheath explants were subjected to a pretreatment under dark condition before they were transferred to the subculture medium for embryo development. Histological and scanning microscopic studies showed that the formation of translucent globular embryogenic cell suspension is an important visible stage during early somatic embryogenesis through indirect method. In the direct pathway, upon pretreatment, epidermal and subepidermal cells of the leaf sheath explants formed globular and elongated structures. The presence of clear protoderm in the globular embryos and procambial structures are crucial visible stages.

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Somatic embryogenesis and plant regeneration in açaí palm (Euterpe oleracea)

Cited by 40 publications

References 26 publications

AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus

AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus

Direct somatic embryogenesis and plant regeneration from leaf sheath explants of mango ginger (Curcuma amada Roxb.)

Direct and Indirect Somatic Embryogenesis in Mango Ginger (Curcuma amada Roxb.)

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