Somatic embryogenesis and plant regeneration from a 700-year-old Kalopanax septemlobus tree

Nyum, Lee Na; Kyu, Moon Heung; Je-Wan, Lee; Eui, Choi Yong; Park, So‐Young

doi:10.1007/s00468-017-1560-4

Cited by 7 publications

(3 citation statements)

References 58 publications

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“…Once tiny shoots emerged, root induction was performed in another MS medium supplemented with 1.2 µM IBA, 2% sucrose, and 0.2% gelrite. These cultures were maintained at 25°C [8,13] with a photoperiod of 16/8 h light/dark during shoot and root development [5].…”

Section: Tissue Culturementioning

confidence: 99%

“…These environmental conditions were gradually introduced to the young regenerants until they were fully acclimated. Subsequently, the plants were placed in larger pots and watered every alternate day to ensure healthy growth [5,13].…”

Section: Tissue Culturementioning

confidence: 99%

“…This technique is useful because it facilitates rapid multiplication of selected plants using in vitro culture techniques [11,12]. Tissue culture-derived micropropagation is more effective than natural and conventional propagation approaches such as seedlings, cuttings, layering, and grafting [1,13]. It has already been used to regenerate medicinal plants, orchids, cereals, vegetables, root crops, and trees [14].…”

Section: Introductionmentioning

confidence: 99%

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Cytogenomic evaluation of regenerated Aralia elata using PLOP-FISH and flow cytometry

Peniton,

Nguyen,

Waminal

et al. 2024

Preprint

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Aralia elata is closely related to Panax ginseng and contains high levels of saponins and other medicinal compounds. Successful A. elata micropropagation is commercially significant; however, the genomic stability of tissue culture-derived regenerants is unclear. In this study, callus-derived regenerated A. elata plants were obtained, and their cytogenomic constitutions were assessed. Using RepeatExplorer, pre-labeled oligonucleotide probes (PLOPs) were developed with newly mined tandem repeats from < 1× NGS whole-genome short reads, fluorescence in situ hybridization (FISH) was performed using six repeat probes, including three universal PLOPs, and genomic DNA content was estimated using flow cytometry. Regenerated A. elata plants (50) exhibited consistent ploidy, repeat distribution, and genome sizes compared with those exhibited by the mother plant. Six repeat probes were detected using FISH. Tandem repeat AeTR49 was identified as an excellent cytogenetic marker for homologous chromosomes, and AeTR161 and AeTR178 were localized in the centromeric and telomeric sections, respectively. Genomic DNA content (2C) was estimated at 2.46 ± 0.04 pg in the mother plant and 2.41 ± 0.05 pg in regenerated plants, with no significant variations in genome size or chromosome length. These results demonstrate that cytogenomics can be used to effectively evaluate chromosome-level genomic stability in regenerated A. elata plants.

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Section: Tissue Culturementioning

confidence: 99%

Section: Tissue Culturementioning

confidence: 99%