Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan

Maruyama, E.; Hosoi, Yoshihisa; Ishii, Keisuke

doi:10.1007/s11627-006-9003-8

Cited by 38 publications

(34 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in our experiments, the EC of Japanese cypresses maintained in the absence of PGR showed a tendency to embryo development and a decline in proliferation over time. This result was consistent with the results reported for other Japanese conifers (Maruyama et al, 2000;Maruyama et al, 2007).…”

Section: Maintenance and Proliferation Of Embryogenic Culturessupporting

confidence: 93%

See 1 more Smart Citation

Somatic Embryogenesis and Efficient Plant Regeneration in Japanese Cypresses

Maruyama¹,

Hosoi²

2012

Embryogenesis

Self Cite

View full text Add to dashboard Cite

Section: Maintenance and Proliferation Of Embryogenic Culturessupporting

confidence: 93%

“…This result indicates that the potential to develop cotyledonary somatic embryos varied among the cell lines. Similar results were reported for the Japanese cedar (Igasaki et al, 2003), maritime pine (Ramarosandratana et al, 2001;Miguel et al, 2004;LeluWalter et al, 2006), and Japanese pines Maruyama et al, 2007).…”

Section: Peg ( Gl -1 ) Somatic Embryossupporting

confidence: 86%

Somatic Embryogenesis and Efficient Plant Regeneration in Japanese Cypresses

Maruyama¹,

Hosoi²

2012

Embryogenesis

Self Cite

View full text Add to dashboard Cite

“…The initial amount of embryogenic tissue usually ranges from 500 mg (Maruyama et al 2007) to 50 mg (Carneros et al 2009) per filter paper disc (7 cm).…”

Section: Discussionmentioning

confidence: 99%

Bottlenecks in Pinus radiata somatic embryogenesis: improving maturation and germination

Montalbán

Diego

Moncaleán

2010

Trees

View full text Add to dashboard Cite

Commercial deployment of clonal trees via somatic embryogenesis (SE) could increase forest productivity over conventional tree breeding techniques. However, some technical advances need to be made to use SE in clonal forestry with Pinus radiata. For example, the conversion of embryonal mass (EM) into plants is at present a major bottleneck. For this reason, maturation experiments were carried out to determine the effect of the initial amount of EM, activated charcoal (AC) and the best combination of abscisic acid (ABA), sucrose and amino acid concentration in the maturation medium. Germination was evaluated on different media formulations with and without AC. When 100 mg of EM were suspended in liquid medium without AC, cotyledonary somatic embryos were obtained in all the maturation media tested. Maturation medium supplemented with 60 lM ABA, 6% sucrose, and embryo development medium amino acid mixture produced the highest number of cotyledonary somatic embryos, between 10 and 1,550 embryos per gram of EM fresh weight. Approximately half of the tested 25 lines produced more than 600 embryos per gFW. Embryo development was the best when somatic embryos were germinated in half strength modified Quoirin and Lepoivre medium supplemented with 2 g L -1 AC. This protocol simplified and improved SE maturation and germination due to the elimination of subcultures, the large number of somatic embryos obtained from a very low amount of EM, and the elimination of pre-germination treatments, resulting in a significant saving of cost and labor.

show abstract

“…e supplement of PEG to the media was essential to obtain high maturation frequencies of somatic embryos as reported for other Japanese pines (Maruyama et al 2005a(Maruyama et al , 2005b(Maruyama et al , 2007a. The stimulating effect of PEG on somatic embryo maturation in conifers has been extensively reported (Gupta and Pullman 1991;Jain et al 1995;Li et al 1997Li et al , 1998Pullman et al 2003;Maruyama et al 2002Maruyama et al , 2005cMaruyama et al , 2007b, and may be related to water stress induction similar to that generated by desiccation and to an increase in the accumulation of storage reserves (Attree et al 1992;Misra et al 1993;Roberts et al 1990a).…”

mentioning

confidence: 94%

Plant regeneration from embryogenic tissue of Pinus luchuensis Mayr, an endemic species in Ryukyu Island, Japan

Hosoi

Maruyama

2012

Plant Biotechnology

Self Cite

View full text Add to dashboard Cite

Somatic embryogenesis in Ryukyumatsu (Pinus luchuensis Mayr.), an endemic species in Ryukyu Island, Japan, was initiated from megagametophytes containing zygotic embryos on a medium supplemented with 10 µM 2,4-dichlorophenoxyacetic acid and 5 µM 6-benzylaminopurine. Embryogenic cultures were maintained and proliferated by subcultures at 2-to 3-week-intervals on the same fresh medium. e maturation of somatic embryos occurred on media containing maltose, activated charcoal, abscisic acid and polyethylene glycol. High frequencies of germination were obtained a er the post-maturation treatment of somatic embryos under conditions of high relative humidity, and around 90% of them were converted into plants. Growth of the somatic embryo-derived regenerated plants has been monitored in the eld.

show abstract

Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan

Cited by 38 publications

References 23 publications

Somatic Embryogenesis and Efficient Plant Regeneration in Japanese Cypresses

Somatic Embryogenesis and Efficient Plant Regeneration in Japanese Cypresses

Bottlenecks in Pinus radiata somatic embryogenesis: improving maturation and germination

Plant regeneration from embryogenic tissue of Pinus luchuensis Mayr, an endemic species in Ryukyu Island, Japan

Contact Info

Product

Resources

About