Somatic Embryogenesis and Plant Regeneration in Suspension Cultures of Dessert (AA and AAA) and Cooking (ABB) Bananas (Musa spp.)

Novák, František; Afza, R.; Duren, M. Van; Perea-Dallos, M.; Conger, B. V.; Xiao-lang, Tang

doi:10.1038/nbt0289-154

Cited by 143 publications

(83 citation statements)

References 17 publications

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“…The choice of auxin is very important in the induction process and can affect the frequency and morphology of somatic embryos (Lazzeri et al 1987, Levi & Sink 1991, Rodriguez & Wetzstein 1994, 1998. Somatic embryogenesis of banana cultivars of different groups has been successfully achieved (Novak et al 1989, Dedh'a et al 1991, Côte et al 1996, Escalant et al 1994, Grapin et al 1996, however, the conversion into plants is frequently low, thus limiting its association with genetic transformation techniques. The characterization of the different stages of the embryogenic process can help to detect possible limiting steps as well as to locate the embryogenic regions in the explant.…”

Section: Introductionmentioning

confidence: 99%

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Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Filippi

Appezzato‐da‐Glória

2001

Rev. bras. Bot.

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-(Histological changes in banana explants, cv. Nanicão (Musa spp. Group AAA), submitted to different auxins for induction of somatic embryogenesis). The present work analyzes the behavior of banana explants, cv. Nanicão (Musa spp. Group AAA) regarding somatic embryogenesis induction treatments with several auxins. Longitudinal segments of shoot meristematic apices of micropropagated banana plantlets cultivated and rooted in vitro were introduced in culture medium containing dicamba, picloram, 2,4-D or NAA in different concentrations. Explant samples were collected at 0, 7 and 10 days and prepared for light microscopy. Histological sections were used for comparison of the histological changes occurring after induction treatment with different auxins. Embryogenic response was observed only in treatments with picloram or dicamba, with distinct embryogenic regions observed at 14 and 21 days in culture, respectively. Histological sections of embryogenic regions of the explant at 26 days in culture revealed the formation of meristematic regions, structures with multiple root meristems, and somatic embryos at early globular stages. Embryo-like structures morphologically similar to Musa balbisiana zygotic embryos were sectioned and showed a lack of apical meristems and absence of procambial differentiation. These results indicate the induction of non-functional somatic embryos and the need for more studies on developmental aspects and maturation treatments for optimization of the process.RESUMO -(Alterações histológicas em explantes de bananeira, cv. Nanicão (Musa spp. Grupo AAA) submetidos a diferentes auxinas para indução de embriogênese somática). O presente trabalho analisa o comportamento de explantes de bananeira 'Nanicão' (Musa spp. Grupo AAA) a tratamentos de indução com diferentes auxinas, visando à obtenção de embriogênese somática. Segmentos longitudinais de ápices meristemáticos caulinares de plântulas de bananeira micropropagadas e enraizadas in vitro foram introduzidos em meio de cultura contendo dicamba, picloram, 2,4-D ou NAA. Amostras dos explantes aos 0, 7 e 10 dias foram coletadas e preparadas para microscopia óptica. Cortes histológicos foram realizados nesses explantes, comparando-se as alterações observadas após os tratamentos com diferentes auxinas. Respostas embriogênicas foram observadas somente nos tratamentos com picloram e dicamba, sendo possível a observação visual de regiões embriogênicas aos 14 e 21 dias de cultivo, respectivamente. Cortes histológicos de regiões embriogênicas dos explantes, aos 26 dias, revelaram a formação de regiões meristemáticas compostas aparentemente por múltiplos meristemas radiculares e embriões nas fases iniciais do estágio globular. Cortes histológicos de estruturas morfologicamente semelhantes a embriões zigóticos de Musa balbisiana revelaram ausência dos meristemas apicais e não diferenciação procambial. Os resultados indicam a indução de embriões somáticos não-funcionais e, consequentemente, a necessidade de maiores estudos visando otimizar o process...

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Section: Introductionmentioning

confidence: 99%

“…cultivars in breeding programs (Novak et al 1989). Somatic embryogenesis associated with genetic manipulation techniques is of great interest for the production of new cultivars through plant transformation.…”

Section: Introductionmentioning

confidence: 99%

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Filippi

Appezzato‐da‐Glória

2001

Rev. bras. Bot.

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“…Meristem culture for clonal propagation and virus eradication was done by Gupta [7] . There are also reports of somatic embryogenesis and regeneration in liquid medium [8][9][10] . Utilization of whole flowers, buds, ovary sections and inflorescence sections as primary explant source material has been reported [11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants

Titov¹,

Bhowmik²,

Mandal³

et al. 2006

American J. of Biochemistry and Biotechnology

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Abstract:The study was undertaken to examine banana cv. Kanthali floral bud apex as an alternative source material for in vitro propagation because huge number of explants die due to microbial contamination in case of shoot tip explants. Contamination free cultures were established by treating the floral bud explants with 0.1% HgCl 2 for 6 min. This study found that inflorescence tissues of experimental plant was almost contamination free but was high in phenolic compounds. Phenolic compounds secretion was successfully stopped by pre-soaking them in an antioxidant solution of 0.125% potassium citrate: citrate. After antioxidant treatment the floral bud explants were cultured on MS medium supplemented with different concentrations and combinations of BA+Kn+IAA/IBA+15%CW. Compact, white/greenish white callus was formed in different amount at all concentrations after 3 weeks of culture. All were again subcultured at same medium and after another 30-35 days at 2.0 mg L 1 BA+2.0 mg L 1 Kn+2.0 mg L 1 IAA+15%CW some callus showed embryogenic structure.

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“…In Musa, SE has been obtained by different ways. They include cultures of zygotic embryos (Cronauer and Krikorian 1988;Escalant and Teisson 1988), leaf tissues and rhizome (Novak et al 1989a), highly proliferating meristems (Dhed'a et al 1991;Schoofs 1997), male inflorescences (Ma 1991;Escalant et al 1994), and female inflorescences (Grapin et al 1998). The most successful explants were the proliferating meristems and male inflorescences.…”

Section: Somatic Embryogenesis In Musa Sppmentioning

confidence: 99%

Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.)

López

Rayas

Santos

et al. 2016

Biotechnologies for Plant Mutation Breeding

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Bananas and plantains (Musa spp.) are among the world's most important crops and are considered as a poor man's crop in tropical and subtropical countries. For this reason, they play a very important role in tropical regions' food security. Several pathogens throughout the tropical areas of the world have become a threat for production, including "black sigatoka" (Mycosphaerella fijiensis Morelet) which is the most damaging disease for Musa plantations in Cuba. It is present in all areas of the country, and it replaced "sigatoka disease" (Mycosphaerella musicola) as the main threat. Chemical control and cultural practices reduce damages, but they are not solving the problem. Genetic variations are the basic tools to develop new cultivars with better traits, such as tolerance against various environmental stresses, resistance against pests and diseases, and improved yield and quality. Tissue culture techniques offer the opportunity for variation induction, handling of large plant populations using established selection methods, and rapidly cloning selected variants. All these strategies can increase the efficiency of mutagenic treatments and subsequent screening of mutant materials. Somatic embryogenesis (SE) is also an excellent system for clonal propagation and mutation induction. The present chapter describes a protocol on how to use embryogenic cell suspensions (ECS) in plantain (Musa spp.) using both in vitro gamma irradiation and plant regeneration in order to achieve genetic improvement. The process involves a series of steps to properly select ECS for irradiation and the posttreatment handling for plant regeneration as well as mutant selection during acclimatization phase and under field conditions.

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Somatic Embryogenesis and Plant Regeneration in Suspension Cultures of Dessert (AA and AAA) and Cooking (ABB) Bananas (Musa spp.)

Cited by 143 publications

References 17 publications

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis

Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants

Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.)

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